RESUMEN
Spontaneous rupture of a common iliac artery aneurysm into the common iliac vein is a rare phenomenon. We report the case of a 68 year old man admitted with acute cardiac failure and massive pulmonary embolism as a complication of a spontaneous ilio-iliac fistula, secondary to aneurysmal rupture. The aneurysm was successfully excluded using an aorto-uni-iliac stent graft. No complications were noted at 9 months follow-up. Arteriovenous fistulae should be considered in patients with aortic or iliac aneurysms who develop a pulmonary embolism or symptoms of venous congestion. Endovascular repair of these pathologies is a feasible therapeutic option; however long term results remain unknown.
Asunto(s)
Aneurisma Roto/cirugía , Fístula Arteriovenosa/cirugía , Implantación de Prótesis Vascular , Procedimientos Endovasculares , Aneurisma Ilíaco/cirugía , Arteria Ilíaca/cirugía , Vena Ilíaca/cirugía , Embolia Pulmonar/etiología , Anciano , Aneurisma Roto/diagnóstico por imagen , Aneurisma Roto/etiología , Fístula Arteriovenosa/diagnóstico por imagen , Fístula Arteriovenosa/etiología , Humanos , Aneurisma Ilíaco/complicaciones , Aneurisma Ilíaco/diagnóstico por imagen , Arteria Ilíaca/diagnóstico por imagen , Vena Ilíaca/diagnóstico por imagen , Masculino , Embolia Pulmonar/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Resultado del TratamientoRESUMEN
Mycobacterium avium subsp. paratuberculosis, an acid-fast bacterium is the agent of Johne's disease, an intestinal disease that causes poor nutrient intake in ruminants. During the period 1987-2003, 322 of 777 (41.4%) goat herds and 97 of 458 (21.1%) sheep flocks were found to be infected with M.a.paratuberculosis in Northern Greece. From goats, mycobacteria were isolated from 238 of 652 (36.5%) of intestinal tissues, 14 of 119 (11.8%) of lymph nodes and five of 369 (1.4%) of faecal samples. From sheep, mycobacteria were isolated from 25 of 162 (15.4%) of intestinal tissues, three of 41 (7.3%) of lymph nodes and two of 322 (0.6%) of faecal samples. Isolates were typed by pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism followed by hybridization to IS900 [IS900- restriction fragment-length polymorphism (RFLP)]. IS900-RFLP BstEII profiles C1 and C5 and PFGE profiles [2-19] and [29-15] were identified. These PFGE profiles have not been found outside Greece to date.
Asunto(s)
Enfermedades de las Cabras/microbiología , Mycobacterium avium subsp. paratuberculosis/clasificación , Paratuberculosis/microbiología , Enfermedades de las Ovejas/microbiología , Animales , Técnicas de Tipificación Bacteriana/veterinaria , Electroforesis en Gel de Campo Pulsado/veterinaria , Heces/microbiología , Enfermedades de las Cabras/epidemiología , Cabras , Grecia/epidemiología , Mycobacterium avium subsp. paratuberculosis/genética , Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación , Paratuberculosis/diagnóstico , Paratuberculosis/epidemiología , Reacción en Cadena de la Polimerasa/veterinaria , Polimorfismo de Longitud del Fragmento de Restricción , Ovinos , Enfermedades de las Ovejas/epidemiologíaRESUMEN
The serological response of young and adult sheep vaccinated conjunctivally with Rev-1 vaccine was assessed by fluorescence polarization assay (FPA), Rose Bengal test (RBT), complement fixation test (CFT), modified Rose Bengal test (m-RBT), indirect ELISA (i-ELISA) and competitive ELISA (c-ELISA), at different post vaccination intervals. One hundred and thirty six adult sheep and 64 lambs were used in the study. The vaccinated animals were bled prior to vaccination (0 day) and thereafter at 21st, 42nd, 35th, 63rd, 91st, 125th, 159th, and 223rd and 330th day post vaccination. The majority of animals (young and adult) showed positive reaction by FPA, RBT, CFT, m-RBT and c-ELISA 21 days post vaccination, whereas by i-ELISA at 42 days. All tests perform equal when animals vaccinated as young are tested 125 days (4 months) post vaccination. In case of animals vaccinated at adulthood, FPA, RBT, CFT and c-ELISA perform equal if the animals are tested 223 days (approximately 8 months) post vaccination. I-ELISA and m-RBT show low specificity if ewes vaccinated at adulthood are tested 330 days (11 months) post vaccination. If control of brucellosis in sheep is based on conjunctivally vaccination of lambs with Rev-1, the vaccinated animals can be tested by any test used for diagnosis of B.melitensis infection accurately at least 4 months post vaccination. If brucellosis control is based on mass vaccination the use of m-RBT and i-ELISA is not recommended for testing adult animals at least for 330 days (11 months) post vaccination due to tests low specificity. Further research is needed so the appropriate cut-offs to be established for FPA, c-ELISA or i-ELISA to become valuable tools for the eradication of Brucella spp. infection in small ruminants in areas where vaccination is practiced.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Vacuna contra la Brucelosis/inmunología , Brucella/inmunología , Brucelosis/veterinaria , Inmunoensayo de Polarización Fluorescente/veterinaria , Enfermedades de las Ovejas/inmunología , Animales , Brucelosis/inmunología , Brucelosis/prevención & control , Femenino , Masculino , Sensibilidad y Especificidad , Pruebas Serológicas/normas , Pruebas Serológicas/veterinaria , Ovinos , Enfermedades de las Ovejas/prevención & control , Factores de Tiempo , Vacunas Atenuadas/inmunologíaRESUMEN
A well-established rabbit model of enteropathogenic E. coli (EPEC) disease was used to examine whether vitamin E (VE) nutritional supplementation had an effect on the pathological changes induced in the bowel by EPEC. Quantitative methods were used to evaluate the influence of VE on bacterial colonization, intestinal mucosal architecture and inflammation, and intestinal epithelial proliferation and apoptosis. VE did not affect EPEC colonization and did not give significant protection against EPEC-induced changes and diarrhoea. Although VE had no effect on the EPEC-related increase of enterocyte apoptosis, it clearly contributed to an acceleration of epithelial cell proliferation in the ileal crypts. This finding may explain why ileal morphometry undertaken in this study showed that VE ameliorated somewhat the effects of EPEC on intestinal mucosal architecture. Quantitative studies on inflammatory cells in the intestinal mucosa revealed that VE nutritional supplementation resulted in an increased neutrophilic and mononuclear inflammatory cell response to EPEC infection, which did not contribute, however, to the clearance of infection.
Asunto(s)
Infecciones por Enterobacteriaceae/tratamiento farmacológico , Escherichia coli/efectos de los fármacos , Íleon/patología , Vitamina E/uso terapéutico , Vitaminas/uso terapéutico , Animales , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Diarrea/microbiología , Diarrea/patología , Suplementos Dietéticos , Modelos Animales de Enfermedad , Infecciones por Enterobacteriaceae/inmunología , Infecciones por Enterobacteriaceae/patología , Enterocitos/efectos de los fármacos , Enterocitos/patología , Escherichia coli/patogenicidad , Femenino , Íleon/efectos de los fármacos , Íleon/microbiología , ConejosRESUMEN
BACKGROUND: Data about the outcome and prognostic factors in the group of patients with non-squamous cell advanced or recurrent carcinomas of the uterine cervix are limited. We compared the outcome of patients with non-squamous with that of squamous cell carcinomas after platinum-based combination chemotherapy as first line therapy for stage IV or recurrent cervical carcinoma. PATIENTS AND METHODS: A total of 200 patients with stage IV or recurrent carcinomas of the cervix received platinum-based combination chemotherapy and were included in our analysis. RESULTS: There were 58 patients with non-squamous and 142 patients with squamous cell carcinomas. Response to chemotherapy was 53.5% in non-squamous vs. 43.5% in squamous carcinomas. Histology was not an independent predictor of tumor response (P = 0.797). Response rates were lower in patients with relapse only in a previously irradiated area in both squamous (26.9% vs. 53.5%, P = 0.005) and non-squamous carcinomas (47.1% vs. 65%, P = 0.270). Weight loss was the only significant predictor of survival in non-squamous histology patients (P < 0.0001). There was no significant difference in median survival between squamous (11.57 months [95% CI 9.35-13.79]) and non-squamous carcinomas (19.05 months [95% CI 13.63-24.47]) (P = 0.064). After adjustment for independent prognostic factors (ECOG performance status and weight loss), differences in survival remained not significant. CONCLUSION: Our study showed a similar outcome for both squamous and non-squamous stage IV or recurrent cervical carcinomas treated with platinum-based combination chemotherapy.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Recurrencia Local de Neoplasia/tratamiento farmacológico , Neoplasias del Cuello Uterino/tratamiento farmacológico , Adenocarcinoma/tratamiento farmacológico , Adenocarcinoma/patología , Adenocarcinoma de Células Claras/tratamiento farmacológico , Adenocarcinoma de Células Claras/patología , Adulto , Anciano , Carcinoma Adenoescamoso/tratamiento farmacológico , Carcinoma Adenoescamoso/patología , Cisplatino/administración & dosificación , Ensayos Clínicos Fase II como Asunto , Ensayos Clínicos Fase III como Asunto , Femenino , Humanos , Persona de Mediana Edad , Recurrencia Local de Neoplasia/patología , Estadificación de Neoplasias , Ensayos Clínicos Controlados Aleatorios como Asunto , Resultado del Tratamiento , Neoplasias del Cuello Uterino/patologíaRESUMEN
Oedema disease usually occurs after weaning and is due to infection with Enterotoxaemic Escherichia coli strains. A total of 240 weaned piglets were used in five groups during a 28-day period. One group (a negative control) was offered feed free of antimicrobials ad libitum, three groups were offered the same diet ad libitum supplemented with either 1.6 per cent lactic acid, 1.5 per cent citric acid or 50 p.p.m. of enrotloxacin (ENR/Baytril I.E.R. 2 5 per cent, Bayer), respectively. Finally, one group was offered the same diet but the amount offered was restricted during the first 12 days post-weaning. Groups receiving acid or ENR additions to the diet had lower mortality than the negative control group (P<0.05). The three groups on treated feed also showed significantly better growth performance and food conversion ratio than the control group (P<0.05). Both organic acids and medication with 50 p.p.m. of ENR for a 10-day period are useful in controlling and/or preventing post-weaning oedema disease.
Asunto(s)
Ácido Cítrico/administración & dosificación , Edematosis Porcina/prevención & control , Infecciones por Escherichia coli/veterinaria , Fluoroquinolonas , Ácido Láctico/administración & dosificación , Alimentación Animal , Animales , Antiinfecciosos/administración & dosificación , Antiinfecciosos/metabolismo , Peso Corporal , Ácido Cítrico/metabolismo , Suplementos Dietéticos , Edematosis Porcina/metabolismo , Edematosis Porcina/patología , Enrofloxacina , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/metabolismo , Infecciones por Escherichia coli/patología , Infecciones por Escherichia coli/prevención & control , Femenino , Intestino Delgado/microbiología , Intestino Delgado/patología , Ácido Láctico/metabolismo , Masculino , Quinolonas/administración & dosificación , Quinolonas/metabolismo , PorcinosRESUMEN
Post-weaning diarrhoea syndrome (PWDS) of piglets is caused mainly by Enterotoxigenic Escherichia coli (ETEC) strains. Six organic acids were tested for their efficacy in the control of PWDS, using a total of 384 weaned piglets, in eight groups, during a 28-day period. One group (negative control) was offered a diet free of antimicrobials, one group (positive control) was offered the same diet medicated with 44 p.p.m. of lincomycin and 44 p.p.m. spectinomycin (Lincospectin 22 premix, Upjohn), and six groups were offered feed supplemented with either 1.0 per cent propionic acid, 1.6 per cent lactic acid, 1.2 per cent formic acid, 1.2 per cent malic acid, 1.5 per cent citric acid or 1.5 per cent fumaric acid. Groups were compared with regard to the appearance of clinical signs, mortality, weight gain and feed conversion. All groups supplemented with organic acids had reduced incidence and severity of diarrhoea, and performed significantly better than the negative control group (P<0.05). At the end of the trial, ETEC strains were detected in the control group not receiving antibiotics but not in the treated group. Organic acids and especially lactic acid are a useful tool in controlling PWDS.
Asunto(s)
Ácidos Carboxílicos/administración & dosificación , Diarrea/veterinaria , Infecciones por Escherichia coli/veterinaria , Enfermedades de los Porcinos/prevención & control , Alimentación Animal , Animales , Peso Corporal , Ácidos Carboxílicos/metabolismo , Diarrea/metabolismo , Diarrea/microbiología , Diarrea/prevención & control , Suplementos Dietéticos , Quimioterapia Combinada/administración & dosificación , Escherichia coli/crecimiento & desarrollo , Infecciones por Escherichia coli/metabolismo , Infecciones por Escherichia coli/prevención & control , Femenino , Lincomicina/administración & dosificación , Masculino , Espectinomicina/administración & dosificación , Porcinos , Enfermedades de los Porcinos/metabolismo , Enfermedades de los Porcinos/microbiologíaRESUMEN
Clostridium perfringens isolated from lambs with dysentery (n=117) were analysed by a DNA amplification technique, the polymerase chain reaction (PCR), in order to determine the prevalence of the alpha-, beta-, beta 2-, epsilon-, iota- and enterotoxin genes. The most prevalent toxin type of C. perfringens found was type B, containing the alpha-, beta-, and epsilon-toxin genes, representing 46% of the cases with clostridial dysentery. C. perfringens type C containing the alpha-, and beta-toxin genes was isolated in 20% and type D, which is characterized by the alpha- and epsilon-toxin genes, was isolated in 28% of all isolates. The recently discovered, not yet assigned beta 2-toxigenic type of C. perfringens was represented in 6% of all isolates. No C. perfringens type A containing the alpha-toxin alone and no type E, which harbours the ADP-ribosylating iota-toxin, were found in the diseased animals. None of the samples contained the enterotoxin gene. Only one type of C. perfringens was found in a given herd, revealing the epidemiological use of PCR toxin gene typing of C. perfringens. The animals originated from 79 different herds with sizes ranging from 30 to 250 animals, bred in the area of northern Greece.
Asunto(s)
Infecciones por Clostridium/veterinaria , Clostridium perfringens/aislamiento & purificación , Disentería/veterinaria , Enterotoxinas/genética , Enfermedades de las Ovejas/microbiología , Animales , Infecciones por Clostridium/microbiología , Clostridium perfringens/genética , ADN Bacteriano/química , Disentería/microbiología , Femenino , Genotipo , Grecia , Masculino , Reacción en Cadena de la Polimerasa/veterinaria , OvinosRESUMEN
OBJECTIVE: To compare a DNA probe test with two cultivation methods for the detection of Mycobacterium avium subsp paratuberculosis in goat and sheep faeces. DESIGN: Comparison of the results of the three methods with histological examination as the reference standard. PROCEDURE: Faecal specimens were obtained from goats and sheep originating from flocks known to be affected with paratuberculosis and tested for Mycobacterium avium subsp paratuberculosis with a DNA probe test and two cultivation methods (old conventional culture and new double incubation method in Herrold's and Lowenstein-Jensen medium). RESULTS: In goats, the sensitivity of the various tests were for the DNA probe test 17.2%, for the double incubation culture method 25.4% and for the old conventional culture method 22.8% using the histopathological results as reference. In sheep the sensitivity of the various tests were for the DNA probe test 13.2%, for the double incubation culture method 8.8% and for the old conventional culture method 5.9% using the histopathological results as reference. The specificity of the above tests was 100% in goats and sheep and the specificity of the double incubation culture method in goats was 91%. CONCLUSIONS: The DNA probe test is a rapid and specific test that could be used in a control program if the sensitivity of the test were improved and possibly in combination with another test.
Asunto(s)
ADN Bacteriano/aislamiento & purificación , Enfermedades de las Cabras/diagnóstico , Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación , Paratuberculosis/diagnóstico , Enfermedades de las Ovejas/diagnóstico , Animales , Técnicas de Cultivo de Célula/veterinaria , Sondas de ADN , Heces/microbiología , Enfermedades de las Cabras/microbiología , Cabras , Mycobacterium avium subsp. paratuberculosis/genética , Sensibilidad y Especificidad , Ovinos , Enfermedades de las Ovejas/microbiologíaRESUMEN
A trial was carried out with HYORESP a Mycoplasma hyopneumoniae (M. hyo) vaccine in order to confirm the benefit of vaccination under field conditions in a commercial industrial farrow-to-finish unit, contaminated with M. hyo. Infection with M. hyo was confirmed through positive blood and colostrum samples [enzyme-linked immunosorbent assay (ELISA) test] combined with positive gross lesions of the lung at slaughter. Two different vaccination schedules were tested. Pigs were randomly allocated to three groups: control non-vaccinated group (n = 130, given a placebo injection at 3, 25 and 70 days of age); early vaccinated group (n = 128, given vaccination at 3 and 25 days of age and a placebo at 70 days of age); late vaccinated group (n = 132, given a placebo at 3 and 25 days of age and vaccination at 70 days of age). Both growth rate and feed conversion ratio were signifcantly (P < 0.05) improved in the vaccinated groups compared with the control group. The lung lesion score was also significantly (P < 0.05) improved in both vaccinated groups. In this trial, it was clearly demonstrated that vaccination is highly effective in improving performance in pig units infected with M. hyo. The improvement in the feed conversion ratio in the vaccinated groups was especially impressive: -0.411 (13% improvement) in the group vaccinated twice at 3 and 25 days of age; -0.162 (5% improvement) in the group vaccinated once at 70 days of age. Performances were better when two shots were given early in life compared with one shot later--probably due to an infection taking place rather early in life for most of the pigs. Moreover, a significant reduction in the cost of supportive (injectable) medication was noticed in vaccinated pigs. In conclusion, HYORESP proved to be a very efficacious tool to control M. hyo in infected herds with its remarkable flexibility that allows the vaccination schedule to be adapted to the specific field conditions.
Asunto(s)
Vacunas Bacterianas/administración & dosificación , Infecciones por Mycoplasma/veterinaria , Mycoplasma/inmunología , Enfermedades de los Porcinos/prevención & control , Factores de Edad , Animales , Vacunas Bacterianas/inmunología , Femenino , Crecimiento , Esquemas de Inmunización , Masculino , Infecciones por Mycoplasma/inmunología , Infecciones por Mycoplasma/prevención & control , Porcinos , Enfermedades de los Porcinos/inmunología , Vacunación/veterinariaRESUMEN
Since there is increasing evidence indicating nitric oxide [NO] would play a role in sepsis, we decided to investigate whether this multifaceted mediator is directly implicated in the process of bacterial translocation. A total of 48 rats received intraperitoneal either Zymosan A (group Z) for systemic inflammation production or sodium chloride solution (controls); they were then further subdivided into three groups of eight animals each, being given, through the tail vein: L-NAME (N-nitro-L-arginine] for inhibition of NO production; SNP (sodium nitroprusside) as NO donor; or sodium chloride as control. After 2 h, the mesenteric lymph node complex was excised, under sterile conditions, and, using standard bacteriological techniques, bacterial translocation was assessed as colony forming units per gram of tissue (CFU/g). Statistical evaluation of the bacteriological data revealed a significant increase of bacterial translocation in all rats subjected to systemic inflammation (group Z) versus controls (P = 0.01) Control rats that were subjected to L-NAME treatment exhibited a statistically significant increase (P = 0.001) in CFU/g compared to sodium chloride treated rats, while SNP treatment revealed no difference in relation to sodium chloride treated rats. Group Z rats, subjected to L-NAME treatment, similarly exhibited a statistically significant increase (P = 0.01) in CFU/g compared to sodium chloride treated rats, while SNP treatment led to a statistical increase of bacterial translocation in relation to sodium chloride treated rats (P = 0.05). The results of this study lead us to suggest that NO appears to participate in the process of bacterial translocation.
Asunto(s)
Traslocación Bacteriana/fisiología , Óxido Nítrico/fisiología , Sepsis/microbiología , Animales , Inflamación/inducido químicamente , Ganglios Linfáticos/microbiología , Masculino , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico/antagonistas & inhibidores , Nitroprusiato/farmacología , Ratas , Ratas Wistar , ZimosanRESUMEN
Post-weaning diarrhoea syndrome (PWDS) of piglets is caused mainly by enterotoxigenic Escherichia coli (ETEC) strains. A new in-feed probiotic, LSP 122 (Alpharma), containing viable spores of Bacillus licheniformis was tested for its efficacy to control PWDS in piglets in a low health-status farm, using four groups with a total of 256 weaned piglets for a 28-day period. One group (negative control) was offered antimicrobial-free and probiotics-free fed, one group was offered feed supplemented with 10(6)viable spores of Bacillus toyoi (Toyocerin(R)) per gram of feed and two groups were offered feed supplemented with 10(6)and 10(7)viable spores of B. licheniformis per gram of feed, respectively, and were compared with regard to the appearance of clinical signs, mortality, weight gain and feed conversion. The results showed that all groups supplemented with probiotics exhibited a reduced incidence and severity of diarrhoea. Mortality in all probiotic supplemented pigs was significantly lower compared with the negative control group (P<0.05). The evaluation of the weight gain data, as well as feed conversion ratio, indicated that the three treated groups performed remarkably better than the negative control group (P<0.05) and the group receiving the high inclusion of LSP 122 performed better than the two other groups receiving probiotics (P<0.05). No ETEC strains were detected on day 22 in the high inclusion of LSP 122 and Toyocerin groups as compared with the untreated control. It was concluded that the high dosage schedule of LSP 122, providing 10(7)viable spores of B. licheniformis per g of feed, is a very useful agent for the control of PWDS due to ETEC.
Asunto(s)
Diarrea/veterinaria , Infecciones por Escherichia coli/veterinaria , Probióticos/uso terapéutico , Enfermedades de los Porcinos/prevención & control , Animales , Diarrea/prevención & control , Infecciones por Escherichia coli/prevención & control , Femenino , Grecia , Masculino , Porcinos , DesteteRESUMEN
Two types of Salmonella abortusovis vaccines were prepared, one with aluminium hydroxide (vaccine A) and the other with water in oil (vaccine B) adjuvants. They were compared in a pregnant mouse model, aiming at protecting them from abortions after challenge with a virulent strain of S. abortusovis. The protection for vaccine A was from 74% to 77.6% and that for vaccine B from 71% to 79.6%. Abortions occurred 5-10 days post challenge and S. abortusovis was isolated from all aborted fetuses and from the liver and the spleen of their mothers at the end of the experiment (18 days post challenge). The presence of salmonella in the liver and the spleen of vaccinated non-pregnant but challenged mice was studied in a separate experiment. The bacterium was isolated from one out of 12 vaccinated mice 6 days post challenge as well as from the six controls.
Asunto(s)
Aborto Veterinario/prevención & control , Vacunas Bacterianas , Salmonelosis Animal/prevención & control , Salmonella/inmunología , Vacunación/veterinaria , Adyuvantes Inmunológicos/normas , Hidróxido de Aluminio/inmunología , Animales , Vacunas Bacterianas/normas , Emulsiones/normas , Femenino , Inyecciones Subcutáneas/veterinaria , Hígado/microbiología , Ratones , Embarazo , Bazo/microbiología , Vacunas de Productos Inactivados/normasRESUMEN
Serological diagnosis of small ruminant abortions due to Salmonella abortusovis (S.a.o) was improved by using modified procedures to produce high titre H-antigens and anti-H serum. While the titres of the two standard antigens were 1:8 and 1:20, both modified antigens had a titre of 1:50. Similarly, the use of adjuvanted and non adjuvanted antigens for the production of anti-H hyperimmune serum resulted in a titre of 1:160,000 as compared to 1:20,000 when standard procedures were used. In addition, the slow micro-agglutination test has been modified and results can now be read within the same day.
Asunto(s)
Aborto Veterinario/diagnóstico , Pruebas de Aglutinación/métodos , Antígenos Bacterianos/inmunología , Salmonelosis Animal/diagnóstico , Salmonella/inmunología , Enfermedades de las Ovejas/diagnóstico , Aborto Veterinario/microbiología , Animales , Anticuerpos Antibacterianos/sangre , Femenino , Embarazo , OvinosRESUMEN
PURPOSE: Both paclitaxel and cisplatin have moderate activity in patients with metastatic or recurrent cancer of the cervix, and the combination of these two agents has shown activity and possible synergism in a variety of solid tumors. We administered this combination to patients with metastatic or recurrent cervical cancer to evaluate its activity. PATIENTS AND METHODS: Thirty-four consecutive patients were treated on an outpatient basis with paclitaxel 175 mg/m2 administered intravenously over a 3-hour period followed by cisplatin 75 mg/m2 administered intravenously with granulocyte colony-stimulating factor support. The chemotherapy was administered every 3 weeks for a maximum of six courses. RESULTS: Sixteen patients (47%; 95% confidence interval, 30% to 65%) achieved an objective response, including five complete responses and 11 partial responses. Responses occurred in 28% of patients with disease within the radiation field only and in 57% of patients with disease involving other sites. The median duration of response was 5.5 months, and the median times to progression and survival for all patients were 5 and 9 months, respectively. Grade 3 or 4 toxicities included anemia in 18% of patients and granulocytopenia in 15% of patients. Fifty-three percent of patients developed some degree of neurotoxicity; 21% of cases were grade 2 or worse. CONCLUSION: The combination of paclitaxel with cisplatin seems relatively well tolerated and moderately active in patients with metastatic or recurrent cervical cancer. The significant incidence of neurotoxicity is of concern, and alternative methods of administration of the two agents could be evaluated. Then, further study of this combination, alone or with the addition of other active agents, is warranted.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Recurrencia Local de Neoplasia/tratamiento farmacológico , Neoplasias del Cuello Uterino/tratamiento farmacológico , Adulto , Anciano , Alopecia/inducido químicamente , Cisplatino/administración & dosificación , Femenino , Factor Estimulante de Colonias de Granulocitos/administración & dosificación , Enfermedades Hematológicas/inducido químicamente , Humanos , Persona de Mediana Edad , Náusea/inducido químicamente , Metástasis de la Neoplasia , Estadificación de Neoplasias , Enfermedades del Sistema Nervioso/inducido químicamente , Paclitaxel/administración & dosificación , Análisis de Supervivencia , Neoplasias del Cuello Uterino/patología , Neoplasias del Cuello Uterino/radioterapiaRESUMEN
Post weaning diarrhoea syndrome (PWDS) and wasting pig syndrome (WPS) are two entities of the pig industry induced by stress factors occurring at the early weaning and further complicated by opportunistic pathogens. In the present paper we examine--through one pilot and one final trial--whether prevention of these two syndromes can be achieved in the field by immunomodulation of the pig e.g., by improving its non-specific immunological response. In both trials two groups of early weaned piglets were submitted to the treatments. In the pilot trial the first group received no treatment and served as controls and the animals of the second group were injected twice with an inactivated Parapoxvirus based immunomodulator (Baypamun, Bayer, Germany). In the final trial the first group of piglets received no treatment and served as a control group. The animals of the second group were injected twice with a freeze-dried immunomodulator (Baypamun). Piglets were compared with regard to the severity of PWS, mortality, growth performance parameters, and for the presence of pathogens in their faeces. The results have shown that Baypamun treated piglets performed significantly better than negative control piglets in all the parameters examined. It was concluded that stimulation of the non-specific immunity of piglets may be helpful in preventing the appearance of PWDS and WPS in the field.
Asunto(s)
Diarrea/veterinaria , Parapoxvirus/inmunología , Infecciones por Poxviridae/veterinaria , Enfermedades de los Porcinos/inmunología , Porcinos/crecimiento & desarrollo , Vacunas de Productos Inactivados , Vacunas Virales , Síndrome Debilitante/veterinaria , Adyuvantes Inmunológicos , Animales , Diarrea/inmunología , Diarrea/prevención & control , Ingestión de Alimentos , Proyectos Piloto , Infecciones por Poxviridae/inmunología , Infecciones por Poxviridae/prevención & control , Enfermedades de los Porcinos/prevención & control , Síndrome Debilitante/inmunología , Síndrome Debilitante/prevención & control , Destete , Aumento de PesoRESUMEN
The aim of this study was to evaluate the effect of different antibiotics used as growth promoters on the control of porcine intestinal adenomatosis when administered in weaning, growing and fattening pig diets, according to Annex I of the European Union directive (70/524/EEC and its subsequent amendments to date) for the use of feed additives. On a farm with a previous history of proliferative enteropathy outbreaks, 648 weaned piglets (23 days old) were divided into nine experimental groups according to bodyweight and sex ratio, each group comprising four pens with 18 pigs in each pen. One group served the trial as a negative (unmedicated) control: another (the positive control) received monensin via feed at 100 p.p.m. up to the end of the growing phase (107 days old) and 50 p.p.m. up to slaughter age (156 days old). The remaining seven groups were offered feed with the addition of the following antibiotics: virginia-mycin (50-20 p.p.m.), avilamycin (40-20 p.p.m.), spiramycin (50-20 p.p.m.), zinc bacitracin (50-10 p.p.m.), avoparcin (40-20 p.p.m.), tylosin (40-20 p.p.m.) and salinomycin (60-30 p.p.m.), respectively. The performance of the pigs in the positive control group was very satisfying and among the highest in the trial, verifying earlier field studies. As a general conclusion it seems that all tested growth promoters had a beneficial effect compared with the untreated control, indicated by the decrease of mortality rate, the elimination of diarrhoeal incidence and the enhancement of growth performance, although the proliferative enteropathy control achieved by each substance was not always satisfactory. More specifically, the antibiotic growth promoters tested can be scaled according to their total efficacy as follows: 1. Salinomycin, tylosin, spiramycin; 2. Virginiamycin, zinc bacitracin, avilamycin; and 3. Avoparcin. Finally, it is considered that part of the growth promotion efficacy of the tested substances is due to their potential capacity to control porcine intestinal adenomatosis; thus, in future growth performance trials, the disease background of the trial farms must be examined, especially for porcine enteropathy challenges.
Asunto(s)
Antibacterianos/uso terapéutico , Profilaxis Antibiótica/veterinaria , Infecciones Bacterianas/veterinaria , Sustancias de Crecimiento/uso terapéutico , Ileítis/veterinaria , Enfermedades de los Porcinos , Porcinos/crecimiento & desarrollo , Mataderos , Animales , Antibacterianos/administración & dosificación , Bacitracina/administración & dosificación , Bacitracina/uso terapéutico , Infecciones Bacterianas/prevención & control , Brotes de Enfermedades/veterinaria , Femenino , Alimentos Fortificados , Glicopéptidos , Grecia/epidemiología , Sustancias de Crecimiento/administración & dosificación , Ileítis/epidemiología , Ileítis/fisiopatología , Ileítis/prevención & control , Íleon , Mucosa Intestinal/microbiología , Mucosa Intestinal/patología , Masculino , Monensina/administración & dosificación , Monensina/uso terapéutico , Oligosacáridos/administración & dosificación , Oligosacáridos/uso terapéutico , Piranos/administración & dosificación , Piranos/uso terapéutico , Espiramicina/administración & dosificación , Espiramicina/uso terapéutico , Enfermedades de los Porcinos/epidemiología , Tilosina/administración & dosificación , Tilosina/uso terapéutico , Virginiamicina/administración & dosificación , Virginiamicina/uso terapéuticoRESUMEN
BACKGROUND: Patients with metastatic carcinoma of the uterine cervix have limited survival. Thus, new chemotherapeutic agents and combinations are needed to improve patient outcome. METHODS: Twenty-seven patients with Stage IV primary or recurrent carcinoma of the uterine cervix were assigned to chemotherapy treatment at 4-week intervals with methotrexate, vinblastine, doxorubicin, and cisplatin (MVAC). The treatment was comprised of methotrexate, 30 mg/m2 administered intravenously (i.v.) on Days 1, 15, and 22; vinblastine, 3 mg/m2 i.v. on Days 2, 15, and 22; doxorubicin, 30 mg/m2 i.v. on Day 2; and cisplatin, 70 mg/m2 i.v. on Day 2. Granulocyte-colony stimulating factor (G-CSF) was given subcutaneously on Days 6-10 at a dose of 5 micrograms/kg. RESULTS: After a median of 4 cycles (a maximum of 6 in responders), the authors observed objective responses in 14 patients (52%), including 3 complete responses (11%) and 11 partial responses (41%). Median overall survival was 11 months (range, 4-15+ months), and median progression free survival of the responders was 8 months (range, 6-15+ months). Toxicity was acceptable and included neutropenia, alopecia, vomiting, and stomatitis. CONCLUSIONS: MVAC is an active regimen in the treatment of patients with advanced or recurrent carcinoma of the uterine cervix. It produced responses in one-half of the patients in this study, and it can be administered on an outpatient basis. The addition of G-CSF appears to reduce hematologic toxicity.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Neoplasias del Cuello Uterino/tratamiento farmacológico , Adulto , Anciano , Cisplatino/administración & dosificación , Doxorrubicina/administración & dosificación , Femenino , Humanos , Metotrexato/administración & dosificación , Persona de Mediana Edad , Recurrencia Local de Neoplasia/tratamiento farmacológico , Estadificación de Neoplasias , Análisis de Supervivencia , Neoplasias del Cuello Uterino/patología , Vinblastina/administración & dosificaciónRESUMEN
Post weaning diarrhoea syndrome (PWDS) in piglets is caused mainly by enterotoxigenic Escherichia (E.) coli (ETEC) strains. Six different in-feed usage programmes of enrofloxacin (ENR/Baytril; I.E.R. 2.5%) were tested for their efficacy on the control of post weaning colibacillosis in piglets, using seven groups with totally 336 weaned piglets for a 28 day period. One group (negative control) was offered feed free of antimicrobials, three groups were offered feed medicated with 50 ppm of ENR starting on weaning day for 5, 7 and 10 days respectively, three groups were offered feed medicated with 50 ppm of ENR starting 7 days post weaning for 5, 7 and 10 days respectively, and were compared with regard to the appearance of clinical signs, mortality, weight gain and feed conversion. The results showed that all ENR treatments reduced the incidence and severity of diarrhoea. Mortality was similar in all ENR treatments, but in the groups where the ENR was added for 10 days immediately after weaning and/or 7 days post weaning for 7 and 10 days respectively was lower compared to the negative control group (P < 0.05). The evaluation of the weight gain data, as well as feed conversion ratio indicated that the six treated groups performed remarkably better than the control group (P < 0.05). No ETEC were detected on days 21 and 28 in all ENR groups contrary to the untreated control. It was concluded that a strategic medication initiated 7 days post weaning with 50 ppm of ENR and only for 7 and 10 days period of time is a useful tool in controlling PWDS due to ETEC.
Asunto(s)
Antiinfecciosos/uso terapéutico , Diarrea/veterinaria , Infecciones por Escherichia coli/veterinaria , Fluoroquinolonas , Quinolonas/uso terapéutico , Enfermedades de los Porcinos/prevención & control , Alimentación Animal , Animales , Antiinfecciosos/administración & dosificación , Diarrea/prevención & control , Enrofloxacina , Infecciones por Escherichia coli/prevención & control , Quinolonas/administración & dosificación , Porcinos , SíndromeRESUMEN
Oedema disease (OD) usually occurs after weaning and is due to infection with (ETEEC) enterotoxaemic Escherichia (E.) coli. This study further examines the efficacy of three different in-feed usage programmes of enrofloxacin (ENR/Baytril I.E.R. 2.5%), on the control of post weaning OD in piglets. The recommended in-feed dosage of ENR for this clinical indication, i.e. 50 p.p.m., was used. Five groups with a total of 240 weaned piglets for 28 days period were used in this trial. One group (negative control) was offered feed free of antimicrobials, one group was offered feed as that of the negative control group except that the feed consumption was restricted for the first 12 days post weaning, and three groups were offered feed ad libitum medicated with 50 p.p.m. of ENR starting 7 days after weaning for 5, 7 and 10 days, respectively, and were compared with regard to their performance. Mortality was lower compared to the negative control group in all ENR treatments (P < 0.05). The evaluation of the growth performance data, as well as feed conversion ratio, indicated that the three treated groups performed remarkably better than the control group (P < 0.05). It was concluded that a strategic medication initiated 7 days post weaning with 50 p.p.m. of ENR and usually for a 10 day period is useful in controlling and/or preventing post weaning OD due to ETEEC.