RESUMEN
20 pairs of sera from the National System of Seroepidemiological Surveillance of the triple viral vaccine received in the laboratory with febrile rash diagnosis were studied. By using the hemagglutination inhibition test, it was observed an abnormal answer of antibodies to both rubella and measles through a falling of the antibody titre in one or both diseases, or in one of them with seroconversion to the other one. With the aim of defining the existence of a possible polyclonal activation already described in literature, it was decided to study the antibody response to family Herpesviridae (HSV, EBV, CMV, VZV). 80% of answer to these viruses were found. The results are submitted and discussed.
Asunto(s)
Anticuerpos Antivirales/inmunología , Infecciones por Herpesviridae/inmunología , Herpesviridae/inmunología , Cápside/inmunología , Preescolar , Citomegalovirus/inmunología , Exantema/virología , Herpesvirus Humano 3/inmunología , Herpesvirus Humano 4/inmunología , Humanos , LactanteRESUMEN
The purification and protein characterization of one of the Cuban isolated strains of hepatitis A virus was carried out. For this, it was necessary to separate the virus from the infected cell by extraction steps with detergents, concentration by ultrafiltration and finally, ultracentrifugation in saccharosoglycerol discontinuous gradient. Protein concentration, as well as the antigenic activity in the different fractions of the gradient were determined. For the protein characterization of the microorganism, those fractions with the greatest specific activity were analyzed by polyacrylamide gel electrophoresis and by Western blotting. It was shown that the viral material was purified and concentrated in the last fractions of the gradient. Bands corresponding to the structural proteins of hepatitis A were observed through electrophoresis and Western blotting.