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BACKGROUND: Although around 1% of cytosines in bees' genomes are known to be methylated, less is known about methylation's effect on bee behavior and fitness. Chemically altered DNA methylation levels have shown clear changes in the dominance and reproductive behavior of workers in queen-less colonies, but the global effect of DNA methylation on caste determination and colony development remains unclear, mainly because of difficulties in controlling for genetic differences among experimental subjects in the parental line. Here, we investigated the effect of the methylation altering agent decitabine on the developmental rate of full bumblebee colonies. Whole genome bisulfite sequencing was used to assess differences in methylation status. RESULTS: Our results showed fewer methylated loci in the control group. A total of 22 CpG loci were identified as significantly differentially methylated between treated and control workers with a change in methylation levels of 10% or more. Loci that were methylated differentially between groups participated in pathways including neuron function, oocyte regulation and metabolic processes. Treated colonies tended to develop faster, and therefore more workers were found at a given developmental stage. However, male production followed the opposite trend and it tended to be higher in control colonies. CONCLUSION: Overall, our results indicate that altered methylation patterns resulted in an improved cooperation between workers, while there were no signs of abnormal worker dominance or caste determination.

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La diferente naturaleza de cada disciplina en triatlón dificulta el consenso en relación a los factores antropométricos óptimos para un alto rendimiento global, especialmente en jóvenes. Por eso, el objetivo fue analizar la correlación de los factores cineantropométricos con el rendimiento observado en los diferentes test. Triatletas infantiles y cadetes (44 masculinos y 20 femeninos) fueron sometidos a una medición antropométrica completa, así como a la evaluación del rendimiento (100 my 400 m en natación, potencia crítica en ciclismo y 1.000 m en carrera). Las variables fueron sometidas a una prueba de normalidad (Shapiro-Wilk) y un análisis correlacional (coeficiente de correlación de Spearman). Los resultados muestran que tanto en el test de 100 m como en el de 400 m, las medidas corporales básicas, los diámetros Biacromial y Biileocrestal, así como los perímetros del brazo, muslo y tórax (perímetros sólo en chicas) tienen las correlaciones más altas con el rendimiento. En el test de ciclismo se observa una correlación moderadamente significativa y negativa (p = -0,556) entre el pliegue de la pierna y la potencia crítica relativa sólo en chicas. Finalmente, el test de carrera a pie correlacionó negativamente con el porcentaje de masa grasa en ambos sexos (Chicos: p = -0,323; chicas: p = -0,646). Estos resultados indican que se deberían tener en cuenta, especialmente, la estatura y la envergadura en el rendimiento en natación, así como el tejido graso en el rendimiento de carrera, especialmente en chicas, por aquellos profesionales que intervienen en el proceso de desarrollo y selección de talento en jóvenes triatletas

The different nature of each discipline in triathlon makes consensus difficult for optimal anthropometric factors for a high global performance, especially in young people. The aim was to analyse the correlation of the cineanthropometric factors with the performance observed in the different test. Young triathletes (44 male and 20 female) were subjected to a full anthropo-metric measurement as well as to the performance assessment (100 m and 400 m in swimming, cycling critical power and 1000 m run). Variables were subject to a normal test (Shapiro-Wilk) and correlational analysis (coefficient of Spearman). The results show that both in the 100 m and 400 m test, basic body measures, Biacromial and Biiliocrestal diameters, as well as arm perimeters, thigh and chest (perimeters only in girls) have the highest correlations with performance. The cycling test shows a moderately significant and negative correlation (p = .556) between the leg fold and the relative critical power only in girls. Finally, run correlated negative to the percentage of fat mass in both sexes (boys: p = -.323; girls: p = -.646). Results indicate that arm span and height should be taken into account in swimming performance, as well as the fat tissue in career performance, especially in girls by professionals involved in the development process and selection of talent in young triathletes

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INTRODUCTION: It has been shown that nutrition plays a crucial role in sport performance, consequently athletes should pay attention to their nutritional habits. However, it is not completely clear what athletes eat just before the sport competition. OBJECTIVES: Analyze the previous energy and nutrient ingestions to a match in soccer players. MATERIAL AND METHOD: Previous intakes from forty-seven players were collected using a 24 hours recall questionnaire. Twenty-four and three hours intakes before the competition were examined using a nutrient ́s composition software. Brand names of commercial food were included. Information concerning time of day, cooking methods and amount of food prepared were collected. Height and weight were measured. Players were asked if they have received nutritional directions in previous seasons. Descriptive statics (mean ± SD) and t-student analyses were used. RESULTS: The mean kcal ingestion was 34.68 ± 16.31 kcal/kg body weight twenty-four hours and 6.89 ± 3.38 kcal/kg body weight three hours before. Carbohydrate average intake was 3.35 ± 1.59 grams/kg body weight twenty-four hours and 0.87 ± 0.43 grams/kg body weight three hours before the match. Proteins mean consumption was 1.49 ± 0.76 grams/kg body weight twenty-four hours and 0.23 ± 0.16 grams/kg body weight three hours before the match. Differences were obtained between players who received nutritional direction and the other players in energy, carbohydrate, proteins and lipids ingested. CONCLUSION: The players studied presented a low kcal and carbohydrate ingestion twenty-four and three hours before a competitive match and they did not fulfill nutritional recommendation. However, nutritional directions could improve pre-vious energy and nutrients intakes

INTRODUCCIÓN: Se ha demostrado que la nutrición juega un papel crucial en el rendimiento deportivo, por ello los deportistas deberían de prestar atención a sus hábitos nutricionales. Sin embargo, no está completamente claro qué es lo que toman los deportistas justamente antes de la competición. OBJETIVOS: Analizar las ingestas previas de energía y nutrientes antes de un partido en jugadores de fútbol. MATERIALES Y MÉTODOS: Se recogieron las ingestas previas de cuarenta y siete jugadores de fútbol usando un cuestionario de 24 horas. Se analizó la ingesta de energía y nutrientes 24 y 3 horas antes del partido utilizando un software de composición nutricional. Se incluyó nombres de marcas comerciales. Se recogió información sobre el horario, los métodos de cocinado y la cantidad de comida preparada. Se midió la altura y el peso de cada jugador. Se les preguntó a los jugadores si habían recibido recomendaciones nutricionales en temporadas anteriores. Se utilizaron métodos estadísticos descriptivos y análisis t-student RESULTADOS: La ingesta calórica media fue de 34,68 ± 16,31 kcal/kg de peso veinticuatro horas antes y 6,89 ± 3,38 kcal/kg peso en las tres horas previas. El consumo medio de carbohidratos fue 3,35 ± 1,59 gramos/kg en las 24 horas y de 0,87 ± 0,43 gramos/kg en las tres horas previas. El consumo de proteínas fue de 1,49 ± 0,76 gramos/kg de peso en el día previo y de 0,23 ± 0,16 gramos/kg en las tres horas anteriores al partido. Se obtuvieron diferencias entre los jugadores que recibieron recomendaciones nutricionales y los que no en las ingestas de energía, carbohidratos, proteínas y lípidos. CONCLUSIÓN: Los jugadores estudiados presentaron una baja ingesta de kcal y carbohidratos en las veinticuatro y en las tres horas anteriores al partido y no cumpliendo con las recomendaciones alimentarias. Sin embargo, recomendaciones nutricionales podrían mejorar la ingesta de energía y nutrientes

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El objetivo de esta investigación es estudiar la eficacia de un programa de tratamiento cognitivo conductual grupal en pacientes con diagnóstico de trastorno adaptativo. La muestra está compuesta por 102 pacientes de dos Unidades de Salud Mental y un centro de Atención Primaria de Valencia (España). El tratamiento consistió en una terapia grupal cognitivo conductual de ocho sesiones de una hora y media de duración, con una frecuencia semanal. Se realizaron mediciones pre y postratamiento y un seguimiento a los tres meses. Los instrumentos que se utilizaron fueron: el "Listado de síntomas revisado" (SCL-90-R), la "Escala de riesgo suicida" (RS) y el "Cuestionario de salud SF-36" (SF-36). Los resultados obtenidos muestran diferencias significativas entre las medidas previas al tratamiento y al finalizarlo en las principales dimensiones de las escalas aplicadas, manteniéndose estos resultados en el seguimiento a los tres meses. Los resultados sugieren que la terapia cognitivo conductual grupal, debido a su eficacia y eficiencia, podría ser utilizada como un primer nivel de atención al trastorno adaptativo

The goal at this research is to study the effectiveness of a cognitive behavioral group therapy program in patients with adjustment disorder. The sample is made up of 102 patients from two Mental Health units and a Primary Care Center in Valencia (Spain). The treatment was a cognitive behavioral group intervention of one hour and a half session per week during eight weeks. Patients were evaluated at the beginning and end of the program. Monitoring was performed at three months. The resources used were: the Symptom Checklist-90-Revised (SCL-90-R), the Suicide Risk Scale (RS) and the SF-36 Health Survey (SF-36). The results show significant differences between previous and following treatment measures on key dimensions of the applied scales, maintaining these results at 3 months follow-up. The results suggest that cognitive-behavioral group therapy could be used as a first level of attention to adjustment disorder because of its effectiveness and efficiency

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Antecedentes: Una rapida recuperacion en los deportistas es un aspecto fundamental para continuar entrenando a intensidades elevadas y seguir progresando mas, especialmente en deportes en los que se compite todoslos dias. Los ejercicios excentricos producen rupturas de miofibrillas musculares, sobre todo si se llevan a cabo de forma intensa y no habitual provocando dano muscular. Este dano muscular produce una fatiga muscular que limita el rendimiento muscular, disminuyendo la fuerza, el pico de potencia, o la velocidad. Por ello, es importante conocer de que medios de recuperación muscular disponen los deportistas. Objetivo: Conocer las causas y consecuencias de la fatiga muscular y hacer una revisión sobre las ayudas ergogenicas: fisicas, nutricionales y farmacologicas que existen para una rapida y mejor recuperacion muscular y orgánica y poder conocer las más eficaces de una manera integral en la práctica deportiva. Métodos: Revisión bibliográfica en distintas bases de datos tanto en lengua castellana como inglesa, siguiendo 4 estrategias especificas, tratando de ofrecer un estado de conocimiento actual sobre las diferentes estrategias de recuperacion post-ejercicio. Resultados: Ciertos deportes, como los de equipo o los que tienen un gran componente excentrico que conllevan una mayor destruccion muscular, requieren una intervencion especial para recuperar los microtraumatismos que se producen durante su practica (entrenamientos y competicion). Hay diferentes formas de mejorar la recuperacion, como métodos fisicos (masaje deportivo, electroestimulacion, contrastes de agua), estrategias nutricionales (hidratacion e ingesta de hidratos de carbonos y proteinas), ergonutricionales (aminoacidos ramificados, glutamina, β-hdroximetil butirato, creatina) y farmacologicas (antiinflamatorios, analgesicos, inmunomoduladores farmacologicos). Estas estrategias pueden ser básicas para conseguir una recuperacion integral del deportista

Background: It is fundamental in athletes a rapid recovery, it is a really important aspect to continue training at high intensities and also to continue progressing, especially in sports when you compete every day. Eccentric exercises produce muscular myofi bril ruptures, especially at high intensities, causing muscle damage. This damage produces muscular fatigue that limits its performance, decreasing the force, the peak power or/and the speed. For that reason, it is important to know the methods to recover from muscle fatigue. Aims: To know the causes and consequences of muscular fatigue and to review the ergogenic aids: physical, nutritional and pharmacological methods for a faster and better recovery. Likewise, to know the most eff ective recovery methods in sports, in a comprehensive way. Methods: Literature review on diff erent databases both Spanish and English, following 4 specifi c strategies, trying to offer a current knowledge about diff erent strategies of post-exercise recovery. Results: Some sports, like team sport or those who have a great eccentric component that involve greater muscle destruction; require special intervention to retrieve traumatisms that occur during sport practice (training and competition). There are different ways to improve it such as physical methods (sports massage, electrical stimulation, water contrasts), nutritional strategies (hydration and carbohydrate and protein intake), ergonutritional (branched chain amino acids, glutamine, β-hidroximetil butyrate, creatine) and pharmacological (analgesic, anti-inflammatory, immunomodulatory drug). These strategies can be basic to achieve a full recovery of the athlete

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Before, during and after physical activity, hydration is a limiting factor in athletic performance. Therefore, adequate hydration provides benefits for health and performance of athletes. Besides, hydration is associated to the intake of carbohydrates, protein, sodium, caffeine and other substances by different dietary aids, during the training and/or competition by athletes. These requirements have led to the development of different products by the food industry, to cover the nutritional needs of athletes. Currently in the European context, the legal framework for the development of products, substances and health claims concerning to sport products is incomplete and scarce. Under these conditions, there are many products with different ingredients out of European Food Safety Authority (EFSA) control where claims are wrong due to no robust scientific evidence and it can be dangerous for the health. Further scientific evidence should be constructed by new clinical trials in order to assist to the Experts Commitees at EFSA for obtaining robust scientific opinions concerning to the functional foods and the individual ingredients for sport population.

Antes, durante y después de la actividad física, la hidratación es un factor limitante en el rendimiento deportivo. Por lo tanto, una adecuada hidratación proporciona beneficios para la salud y el rendimiento de los deportista. Además, la hidratación se asocia a la ingesta de hidratos de carbono, proteínas, sodio, cafeína y otras sustancias durante el entrenamiento y/o competición de los deportistas. Estos requisitos han llevado al desarrollo de diferentes productos por parte de la industria alimentaria, para cubrir las necesidades nutricionales de los deportistas. Actualmente en el contexto europeo, el marco legal para el desarrollo de productos, sustancias y declaraciones nutricionales y de propiedades saludables relativas a productos deportivos, es incompleta y escasa. Hay muchos productos con diferentes ingredientes controlados por la Agencia Europea de Seguridad Alimentaria (EFSA), los cuales poseen declaraciones nutricionales y de propiedades saludables erróneas, debido a la falta o escasa evidencia científica, resultado peligroso para la salud. Se necesita mayor evidencia científica obtendia a través de nuevos ensayos clínicos con el fin de ayudar a los Comités de expertos de la EFSA para la obtención de dictámenes científicos sólidos relativos a los alimentos funcionales y los ingredientes individuales para la población deportiva.

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Before, during and after physical activity, hydration is a limiting factor in athletic performance. Therefore, adequate hydration provides benefits for health and performance of athletes. Besides, hydration is associated to the intake of carbohydrates, protein, sodium, caffeine and other substances by different dietary aids, during the training and/or competition by athletes. These requirements have led to the development of different products by the food industry, to cover the nutritional needs of athletes. Currently in the European context, the legal framework for the development of products, substances and health claims concerning to sport products is incomplete and scarce. Under these conditions, there are many products with different ingredients out of European Food Safety Authority (EFSA) control where claims are wrong due to no robust scientific evidence and it can be dangerous for the health. Further scientific evidence should be constructed by new clinical trials in order to assist to the Experts Commitees at EFSA for obtaining robust scientific opinions concerning to the functional foods and the individual ingredients for sport population (AU)

Antes, durante y después de la actividad física, la hidratación es un factor limitante en el rendimiento deportivo. Por lo tanto, una adecuada hidratación proporciona beneficios para la salud y el rendimiento de los deportista. Además, la hidratación se asocia a la ingesta de hidratos de carbono, proteínas, sodio, cafeína y otras sustancias durante el entrenamiento y/o competición de los deportistas. Estos requisitos han llevado al desarrollo de diferentes productos por parte de la industria alimentaria, para cubrir las necesidades nutricionales de los deportistas. Actualmente en el contexto europeo, el marco legal para el desarrollo de productos, sustancias y declaraciones nutricionales y de propiedades saludables relativas a productos deportivos, es incompleta y escasa. Hay muchos productos con diferentes ingredientes controlados por la Agencia Europea de Seguridad Alimentaria (EFSA), los cuales poseen declaraciones nutricionales y de propiedades saludables erróneas, debido a la falta o escasa evidencia científica, resultado peligroso para la salud. Se necesita mayor evidencia científica obtenida a través de nuevos ensayos clínicos con el fin de ayudar a los Comités de expertos de la EFSA para la obtención de dictámenes científicos sólidos relativos a los alimentos funcionales y los ingredientes individuales para la población deportiva (AU)

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The present study established the pattern of isoprostanes (IsoPs) and prostaglandins metabolites (PGMs) in urine after triathlon training. Fifteen Caucasian triathletes - 5 women and 10 men - performed 793 and 1603 Objective Load Scales, respectively. The optimization of urine hydrolysis conditions, concerning to the type of buffer, the units of hydrolytic enzyme added, and the pH, allowed precise quantification of these metabolites by UPLC-MS/MS, avoiding the under-estimation of their concentrations that occurred in previous studies. Their rate of conjugation ranged between 36% and 100%. This implies significant importance since it supposes non-detection of some IsoPs and PGMs totally conjugated with glucuronic acid developed by other previous methodologies. Among the 13 compounds analyzed, this assay detected and characterized 4 IsoPs and 3 PGMs in the triathletes' urine. The PGMs tetranor-PGEM and 11ß-PGF(2α) and the IsoP 8-iso-PGF(2α), showed lower concentrations after the training program, whereas the PGMs 6-keto-PGF(1α) increased (vascular PGI(2) metabolite). In fact, their pattern in the triathletes' urine indicated that their variation may have been related with the physical activity. Due to its high variation, 6-keto PGF(1α) stood out as a useful marker of the vasodilation and inhibition of the platelet aggregation of the PGI(2) linked to the physical exercise. The data obtained provided a global picture of changes in lipid peroxidation and vascular events as a consequence of chronic exercise.

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Hollow magnetite microspheres have been synthesized by a simple process through a template-free hydrothermal approach. Hollow microspheres were surface modified by coating with a silica nanolayer. Pristine and modified hollow microparticles were characterized by field-emission electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, FT-IR and Raman spectroscopy, and VSM magnetometry. The potential application of the modified hollow magnetite microspheres as a drug carrier was evaluated by using Rhodamine B and methotrexate as model drugs. The loading and release kinetics of both molecules showed a clear pH and temperature dependent profile. GRAPHICAL Hollow magnetite microspheres have been synthesized. Load-release experiments with Rhodamine-B as a model drug and with Methotrexate (chemotherapy drug used in treating certain types of cancer) demonstrated the potential applications of these nanostructures in biomedical applications.

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The present study was performed to analyze in detail gender- and site-related alterations in the adrenergic signal transduction pathway of lipolysis in fat cells isolated from subcutaneous abdominal and visceral fat depots from severely obese patients. The study group consisted of 30 morbidly obese subjects (9 men and 21 women) aged 41.1+/-1.9 years, with a body mass index (BMI) of 54.7+/-1.7 kg/m2, who had undergone abdominal surgery. Protein levels of hormone-sensitive lipase (HSL) and adrenergic receptors (AR), as well as HSL activity and the lipolytic response to adrenergic agents were analyzed. Both fat depots had similar basal lipolysis, but the capacity of catecholamines to activate lipolysis was greater in visceral fat, both at AR and postreceptor levels. Basal lipolysis and lipolytic activity induced by dibutyryl cyclic AMP were higher in men than in women. However, the visceral depot of women showed a higher maximal stimulation by noradrenaline than that of men, in accordance with higher beta1- and beta3-AR protein levels. In conclusion, the main gender-related differences were located in the visceral depot, with women exhibiting a higher sensitivity to catecholamines associated with an increased provision of beta-AR, while men showed an enhanced lipolytic capacity at the postreceptor level.

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When the frenzied and irregular food-recruitment dances of bumblebees were first discovered, it was thought that they might represent an evolutionary prototype to the honeybee waggle dance. It later emerged that the primary function of the bumblebee dance was the distribution of an alerting pheromone. Here, we identify the chemical compounds of the bumblebee recruitment pheromone and their behaviour effects. The presence of two monoterpenes and one sesquiterpene (eucalyptol, ocimene and farnesol) in the nest airspace and in the tergal glands increases strongly during foraging. Of these, eucalyptol has the strongest recruitment effect when a bee nest is experimentally exposed to it. Since honeybees use terpenes for marking food sources rather than recruiting foragers inside the nest, this suggests independent evolutionary roots of food recruitment in these two groups of bees.

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The development of metabolic complications of obesity has been associated with the existence of depot-specific differences in the biochemical properties of adipocytes. The aim of this study was to investigate, in severely obese men and women, both gender- and depot-related differences in lipoprotein lipase (LPL) expression and activity, as well as the involvement of endocrine and biometric factors and their dependence on gender and/or fat depot. Morbidly obese, nondiabetic, subjects (9 men and 22 women) aged 41.1+/-1.9 years, with a body mass index (BMI) of 54.7+/-1.7 kg/m(2) who had undergone abdominal surgery were studied. Both expression and activity of LPL and leptin expression were determined in adipose samples from subcutaneous and visceral fat depots. In both men and women, visceral fat showed higher LPL mRNA levels as well as lower ob mRNA levels and tissue leptin content than the subcutaneous one. In both subcutaneous and visceral adipose depots, women exhibited higher protein content, decreased fat cell size and lower LPL activity than men. The gender-related differences found in abdominal fat LPL activity could contribute to the increased risk for developing obesity-associated diseases shown by men, even in morbid obesity, in which the massive fat accumulation could mask these differences. Furthermore, the leptin content of fat depots as well as plasma insulin concentrations appear in our population as the main determinants of adipose tissue LPL activity, adjusted by gender, depot and BMI.

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