RESUMEN
In this work, silica nanospheres were used as support for gold nanoparticles and applied for bisphenol A electrochemical detection. The development of new silica-supported materials has attracted increasing attention in the scientific world. One approach of interest is using silica nanospheres as support for gold nanoparticles. These materials have a variety of applications in several areas, such as electrochemical sensors. The obtained materials were characterized by solid-state UV-vis spectroscopy, electron microscopy, X-ray diffraction, and electrochemical techniques. The electrode modified with AuSiO2700/CHI/Pt was applied as an electrochemical sensor for BPA, presenting an oxidation potential of 0.842 V and a higher peak current among the tested materials. The AuSiO2700/CHI/Pt electrode showed a logarithmic response for the detection of BPA in the range of 1-1000 nmol L-1, with a calculated detection limit of 7.75 nmol L-1 and a quantification limit of 25.8 nmol L-1. Thus, the electrode AuSiO2700/CHI/Pt was presented as a promising alternative to an electrochemical sensor in the detection of BPA.
RESUMEN
The wide use of chitosan microspheres in pharmaceutical applications, mainly in the controlled release of drugs and as a bactericidal agent, has been widely reported in the literature. However, these important biomaterial applications with multifunctionality is still scarce. In this study, epichlorohydrin-crosslinked chitosan microspheres were prepared and evaluated for Ag+ adsorption and formation of silver nanoparticles. The hybrid material obtained was used to antibacterial activity and controlled drug release. The optimal pH for adsorption of Ag+ ions in aqueous medium was pH 5-7; the best fit was the Langmuir model; the optimal time for maximum adsorption was 10 h. The highest release of the drug occured at neutral pH of the intestinal fluids and remained constant for 6 h. Silver ion microspheres demonstrated activity against E. coli and S. aureus. The concentration of ibuprofen incorporated in the chitosan microspheres was 7.9 × 10-3 mol L-1 and in the microspheres with AgNPs the concentration was 1.8 × 10-2 mol L-1. The microspheres with AgNPs released more drug (77%) than the material without AgNPs.
Asunto(s)
Antibacterianos/farmacología , Quitosano/química , Ibuprofeno/farmacología , Plata/química , Adsorción , Antibacterianos/química , Preparaciones de Acción Retardada , Epiclorhidrina , Escherichia coli/efectos de los fármacos , Concentración de Iones de Hidrógeno , Ibuprofeno/química , Nanopartículas del Metal , Pruebas de Sensibilidad Microbiana , Microesferas , Estructura Molecular , Plata/farmacocinética , Staphylococcus aureus/efectos de los fármacosRESUMEN
Nano-emulsions are promising carriers for antigen delivery. Here, we evaluated the efficacy of a water-oil nano-emulsion containing concentrated, inactivated Clostridium novyi (C. novyi) type B supernatant culture (nano-iCnB) in protecting Swiss mice against a lethal dose of alpha toxin concentrated extract. Proteins were confirmed in the nano-iCnB and their stabilities were determined according physical parameters such as Zeta Potential (ZP). Biochemical, hematological parameters and morphological appearance of liver, spleen and thigh muscle alterations were examined to determine the safety of the compound. Partial protection against lethal doses was achieved in immunized mice despite low IgG titers. These data suggest that our nano-emulsion is a simple and efficient method of promoting antigen delivery for toxin-related diseases.