RESUMEN
Human respiratory syncytial virus (HRSV) is an important cause of respiratory disease. The majority of studies addressing the importance of virus co-infections to the HRSV-disease have been based on the detection of HRSV by RT-PCR, which may not distinguish current replication from prolonged shedding of remnant RNA from previous HRSV infections. To assess whether co-detections of other common respiratory viruses are associated with increased severity of HRSV illnesses from patients who were shedding viable-HRSV, nasopharyngeal aspirates from children younger than 5 years who sought medical care for respiratory infections in Ribeirão Preto (Brazil) were tested for HRSV by immunofluorescence, RT-PCR and virus isolation in cell culture. All samples with viable-HRSV were tested further by PCR for other respiratory viruses. HRSV-disease severity was assessed by a clinical score scale. A total of 266 samples from 247 children were collected and 111 (42%) were HRSV-positive. HRSV was isolated from 70 (63%), and 52 (74%) of them were positive for at least one additional virus. HRSV-positive diseases were more severe than HRSV-negative ones, but there was no difference in disease severity between patients with viable-HRSV and those HRSV-positives by RT-PCR. Co-detection of other viruses did not correlate with increased disease severity. HRSV isolation in cell culture does not seem to be superior to RT-PCR to distinguish infections associated with HRSV replication in studies of clinical impact of HRSV. A high rate of co-detection of other respiratory viruses was found in samples with viable-HRSV, but this was not associated with more severe HRSV infection.
Asunto(s)
Coinfección/virología , Virus ARN/aislamiento & purificación , Infecciones del Sistema Respiratorio/virología , Virosis/virología , Brasil , Preescolar , Coinfección/patología , Femenino , Técnica del Anticuerpo Fluorescente , Humanos , Lactante , Recién Nacido , Masculino , Nasofaringe/virología , Infecciones del Sistema Respiratorio/patología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Índice de Severidad de la Enfermedad , Cultivo de Virus , Virosis/patologíaRESUMEN
External cervical resorption (ECR) is a sequela of dentoalveolar trauma that may cause functional, esthetic, and psychologic alterations. The aim of this study was to report a successful multidisciplinary treatment approach performed in a 12-year-old patient who presented with posttraumatic ECR associated with extensive opened cavity, pulp necrosis, and periapical lesion of tooth number 9, with an initial unfavorable prognosis. Crown lengthening was done to enable restoration of vestibular surface with resin composite, forming a barrier that allowed endodontic treatment. Afterwards, a prefabricated fiberglass post was cemented and esthetic restoration was performed using the adhesive technique and direct composite veneer. Reconstructive periodontal surgery was performed to correct irregular gingival contour. After treatment and successive follow-up sessions, it was concluded that although the tooth had been indicated for extraction, low invasive direct techniques were effective to recover function and esthetics and to maintain the tooth in the oral cavity.
Asunto(s)
Incisivo/lesiones , Grupo de Atención al Paciente , Resorción Radicular/terapia , Niño , Resinas Compuestas/química , Alargamiento de Corona/métodos , Caries Dental/complicaciones , Materiales Dentales/química , Necrosis de la Pulpa Dental/complicaciones , Restauración Dental Permanente/métodos , Coronas con Frente Estético , Estética Dental , Femenino , Estudios de Seguimiento , Gingivoplastia/métodos , Vidrio/química , Humanos , Planificación de Atención al Paciente , Enfermedades Periapicales/complicaciones , Técnica de Perno Muñón/instrumentación , Tratamiento del Conducto Radicular/métodos , Fracturas de los Dientes/complicaciones , Resultado del TratamientoRESUMEN
Imipenem-resistant Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia. Aiming to determine the risk factors associated for hospital-acquired pneumonia due to imipenem-resistant Pseudomonas aeruginosa, we undertook a retrospective case-case-control study. Patients admitted to a 14-bed medical-surgical intensive care unit from a university-affiliated hospital with hospital-acquired pneumonia caused by imipenem-resistant Pseudomonas aeruginosa strains and by imipenem-susceptible Pseudomonas aeruginosa strains were matched to control patients by time under risk and comorbidities. A total of 58 resistant cases, 47 susceptible cases and 237 controls were evaluated. The risk factors independently associated to hospital-acquired pneumonia caused by imipenem-resistant Pseudomonas aeruginosa were: duration of hospitalisation, Acute Physiological and Chronic Health Evaluation II score, male gender receipt of haemodialysis, receipt of piperacillin-tazobactam and receipt of third-generation cephalosporins.
Asunto(s)
Antibacterianos/farmacología , Infección Hospitalaria/etiología , Imipenem/farmacología , Unidades de Cuidados Intensivos , Neumonía Bacteriana/etiología , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/efectos de los fármacos , Estudios de Casos y Controles , Infección Hospitalaria/microbiología , Farmacorresistencia Bacteriana , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neumonía Bacteriana/microbiología , Factores de RiesgoRESUMEN
The epidermal proteins from staged Apis mellifera pupae and pharate adults and the progress of cuticular pigmentation until adult eclosion were used as parameters to study integument differentiation under hormonal treatment. Groups of bees were treated at the beginning of the pupal stage with the juvenile hormone analog pyriproxyfen (PPN) or as pharate adults with 20-hydroxyecdysone (20E). Another group was treated with both hormones applied successively at these same developmental periods. Controls were maintained without treatment. The epidermal proteins, separated by SDS-PAGE and identified by silver staining, were studied at seven intervals during the pupal and pharate adult stages. The initiation and progress of cuticular pigmentation was also monitored and compared to controls. The results showed that PPN reduced the interval of expression of some epidermal proteins, whereas 20E had an antagonistic effect, promoting a prolongation in the time of expression of the same proteins. In PPN-treated bees, cuticular pigmentation started precociously, whereas in 20E-treated individuals this developmental event was postponed. The double hormonal treatment restored the normal progress of cuticular pigmentation and, to a large extent, the temporal epidermal protein pattern. These results are discussed in relation to the 20E titer modulation and morphogenetic hormone interaction.
RESUMEN
O objetivo das equipes volantes de Saúde é dar atendimento a populaçäo da zona rural do Município de Säo Paulo. A equipe volante é constituída por um médico, um auxiliar de enfermagem, um atendente de enfermagem e um motorista, sendo o transporte da mesma efetuado por veículo tipo furgäo que leva também vacinas, medicamentos, suplemento alimentar, material e prontuários necessários ao atendimento. Os locais de atendimento säo fixos, indicados e cedidos pela comunidade, obedecendo a uma frequência semanal de 1 até 3 vezes por semana, de acordo com a demanda. Esse projeto foi implantado em dezembro de 1980 e vem sendo avaliado continuamente