RESUMEN
The Culex pipiens complex includes two widespread mosquito vector species, Cx. pipiens and Cx. quinquefasciatus. The distribution of these species varies in latitude, with the former being present in temperate regions and the latter in tropical and subtropical regions. However, their distribution range overlaps in certain areas and interspecific hybridization has been documented. Genetic introgression between these species may have epidemiological repercussions for West Nile virus (WNV) transmission. Bayesian clustering analysis based on multilocus genotypes of 12 microsatellites was used to determine levels of hybridization between these two species in Macaronesian islands, the only contact zone described in West Africa. The distribution of the two species reflects both the islands' biogeography and historical aspects of human colonization. Madeira Island displayed a homogenous population of Cx. pipiens, whereas Cape Verde showed a more intriguing scenario with extensive hybridization. In the islands of Brava and Santiago, only Cx. quinquefasciatus was found, while in Fogo and Maio high hybrid rates (â¼40%) between the two species were detected. Within the admixed populations, second-generation hybrids (â¼50%) were identified suggesting a lack of isolation mechanisms. The observed levels of hybridization may locally potentiate the transmission to humans of zoonotic arboviruses such as WNV.
RESUMEN
Culex pipiens molestus is an autogenous mosquito, a vector of Dirofilaria immitis in Madeira Island, and it mounts a melanotic encapsulation response when inoculated intrathoracically with D. immitis microfilariae (mf). Because Cx. p. molestus is autogenous, this mosquito is a good model from which to gain a better understand the relationship between oviposition and melanization, independent of the signaling pathways related to blood feeding. The present work assessed the impact follicle growth might have on melanization of intrathoracically inoculated mf. The ovaries from mosquitoes undergoing melanotic encapsulation developed more eggs than those that could not melanize the mf. Possible explanations are discussed in this article.
Asunto(s)
Culex/inmunología , Culex/parasitología , Dirofilaria immitis/fisiología , Interacciones Huésped-Parásitos/inmunología , Insectos Vectores/inmunología , Insectos Vectores/parasitología , Animales , Dirofilaria immitis/inmunología , Perros , Femenino , Melaninas/metabolismo , Oviposición/inmunología , Factores de TiempoRESUMEN
Field and laboratory studies were performed to verify whether Culex theileri Theobald functions as a natural vector of Dirofilaria immitis (Leidy) on Madeira Island, Portugal. CO2-baited light traps (EVS traps) were use to sample mosquitoes monthly basis between February 2002 and February 2003 in the area of Quebradas (Funchal). Three mosquito species were captured, including 58 Culex pipiens L., 790 Cx. theileri, and three Culiseta longiareolata (Macquart). Only C. theileri tested positive for D. immitis. The presence of this filarial worm was detected by direct observation, infectivity assay dissection technique, and polymerase chain reaction methods. Infected mosquitoes were recovered in October and December 2002 and January 2003. These data provide evidence that Cx. theileri could be the main vector of D. immitis in Funchal, Madeira.