RESUMEN

Identification of ophidian paramyxovirus (OPMV) nucleic acid was accomplished in 11 of 14 snakes by a reverse transcriptase-polymerase chain reaction (RT-PCR) assay that detected a 153-bp region of the OPMV genome in total RNA extracted from paraffin-embedded tissues and cell culture. The RT-PCR protocol amplified a portion of the OPMV RNA genome, producing a 153-bp complementary DNA (cDNA) product from both fresh and paraffin-embedded tissue samples. In addition, cDNA:RNA in situ hybridization localized OPMV in formalin-fixed, paraffin-embedded tissue specimens to specific tissues and cells. This latter technique increased the degree of specificity with which a diagnosis of OPMV could be made.

Asunto(s)

Hibridación in Situ/veterinaria , Infecciones por Paramyxoviridae/veterinaria , Paramyxoviridae/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Serpientes/virología , Animales , Encéfalo/virología , ADN Complementario/química , ADN Complementario/genética , Hibridación in Situ/métodos , Riñón/virología , Pulmón/virología , Paramyxoviridae/genética , Infecciones por Paramyxoviridae/diagnóstico , Infecciones por Paramyxoviridae/virología , ARN Viral/química , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos