RESUMEN
Treatment of wheat leaves with heptanoyl salicylic acid (HS) and trehalose at concentrations of 0.1 and 15 g l(-1), prior to fungal inoculation, resulted in 40% and 60% protection, respectively, against powdery mildew. The total lipid composition of Blumeria graminis f.sp. tritici (Bgt) conidia, the causal agent of wheat powdery mildew, was compared when produced on wheat leaves, respectively, untreated and treated with the two elicitors, HS and trehalose. An obvious effect was observed on lipid composition (sterol and fatty acid (FA)) of Bgt conidia produced on wheat leaves treated with HS. A total of 16 FA (C12-C24 saturated and unsaturated) as well as unusual methoxylated Fatty Acids (mFA) (3-methoxydocosanoic and 3-methoxytetracosanoic acids) were detected in the conidia. Medium chain FA were predominant in HS treated conidia (64.65%) while long chain fatty acids constituted the major compounds in untreated conidia (62%). The long chain/medium chain FA ratio decreased from 1.8 in the conidia produced on untreated leaves to 0.5 in the conidia obtained from HS treated leaves. When comparing the sterol composition of Bgt conidia produced on leaves treated with HS versus conidia obtained from untreated ones, very important changes within the two major classes can be seen. In particular, 24-methylsterols, e.g., 24-methylenecholesterol and 24-methylcholesta-7,24-dien were reduced by about 82% whereas 24-ethylsterols, e.g., 24-ethylcholesterol and 24-ethylcholesta-5,22-dienol were increased by about 85%. The 24-methylsterols/24-ethylsterols ratio was reduced by ninefold in the conidia produced from HS treated leaves.
Asunto(s)
Ascomicetos/química , Ascomicetos/efectos de los fármacos , Fungicidas Industriales/farmacología , Lípidos/análisis , Hojas de la Planta/microbiología , Triticum/microbiología , Fungicidas Industriales/química , Estructura Molecular , Hojas de la Planta/química , Salicilatos , Ácido Salicílico/química , Ácido Salicílico/farmacología , Trehalosa/farmacología , Triticum/químicaRESUMEN
Plant-pathogen interactions are still classically described using concepts that make a distinction between qualitative and quantitative aspects linked to these concepts. This article first describes these aspects, using the terminology associated with them. It then presents some recent experimental observations that demonstrate that such concepts share either common or closely related mechanisms at the cellular and molecular levels. The emergence of a more global vision and understanding of the interactions between plants and their parasites is discussed.
Asunto(s)
Eucariontes/patogenicidad , Plantas/parasitología , Animales , Genes de Plantas , Interacciones Huésped-Parásitos , Inmunidad Innata , Modelos Biológicos , Células Vegetales , Enfermedades de las Plantas/parasitología , Plantas/genética , Especificidad de la EspecieRESUMEN
The total fatty acids (FA) composition of Blumeria graminis f.sp. tritici conidia, the causal agent of wheat powdery mildew, was analyzed as a function of their age. A total of 19 FA (C12-C24 saturated and unsaturated) and unusual methoxylated fatty acids (mFA) were detected in young, intermediate and old conidia. Two very long chain methoxylated FA were identified by GC-MS as 3-methoxydocosanoic and 3-methoxytetracosanoic acids. Medium chain FA were predominant in young conidia (75%, including 13% of mFA) while very long chain fatty acids constituted the major compounds in old conidia (74%, including 30% of mFA). We have shown for the first time that the total FA composition is strongly correlated with the age of B. graminis f.sp. tritici (Bgt) conidia.