RESUMEN
Insulin injections cause an increase of the total content of saturated fatty acids in the sphingomyelin and phosphatidylethanolamine fractions of rat skeletal muscles as well as in the amount of unsaturated fatty acids in the phosphatidyl choline, phosphatidyl serine and phosphatidyl inositol fractions. These effects are not always opposite in case of alloxan diabetes.
Asunto(s)
Diabetes Mellitus Experimental/metabolismo , Ácidos Grasos/metabolismo , Insulina/farmacología , Músculos/metabolismo , Fosfolípidos/metabolismo , Animales , Insulina/metabolismo , Masculino , RatasRESUMEN
The contents of individual gangliosides in various tissues (e. g. heart, skeletal muscles, liver, brain, kidney, spleen, pancreas) under normal conditions and in alloxan diabetes as well as the incorporation of 1-14C-stearate into the phospholipid, glyceride and total ganglioside fractions were studied. The oxidation of 14C-fatty acid and the activities of neuraminidase and lipase in these tissues were determined. It was found that diabetes is concomitant with a marked decrease of the content and exchange of gangliosides, which is paralleled with activation of neuraminidase. The incorporation of 14C-stearate in the sphingomyelin and phosphatidylcholine fractions is significantly decreased, while that in the cardiolipin fraction is increased 1.5 - 2-fold. The oxidation of 14C-stearate is also increased, the degree of this increase being tissue-specific.