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Objective: Assess the perceived protection afforded by a range of COVID-19 vaccines in immune-mediated inflammatory diseases patients previously vaccinated against SARS-CoV-2. Study design: Survey. Methods: On-line cross-sectional survey aimed at evaluating the perceived protection (and its determinants) afforded by a range of COVID-19 vaccines among immune-mediated inflammatory diseases previously vaccinated for COVID-19. Results: Out of 493 eligible respondents who lived in Brazil, 397 (80.5%) were confident that their primary vaccination series would protect them against severe COVID-19. In multivariate analysis, only overlapping immune-mediated inflammatory diseases remained (negatively) associated with the perception of protection. Conclusions: No influence was found between COVID-19 vaccine types and the perception of protection after initial vaccinations.

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Genetic diversity among local accessions and varieties subsidize plant breeding programs, allowing the utilization of existing variability in plants that have already adapted to local climate conditions. An alternative to studying genetic variability is the study of diversity. The aim of this research was to study genetic diversity among sugarcane accessions and varieties used for the production of craft-distilled cachaça (distilled sugarcane alcohol) in the region of Lavras, Minas Gerais, Brazil. Using a one-way design, an experiment was conducted in the municipality of Perdões, Minas Gerais to evaluate 35 regional accessions derived from germplasm collection expeditions and four varieties. Using morphological descriptions of 46 multicategorical sugarcane characteristics, dissimilarity and Tocher cluster method analyses were performed. Based on the results, it was concluded that genetic diversity exists among the accessions evaluated for the target traits.

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Los pesticidas pueden producir efectos agudos o crónicos en la salud humana. Muchos de ellos pueden provocar daño en el material genético. Esta modificación en la información genética se ha relacionado con un alto riesgo de padecer cáncer. El objetivo del presente trabajo es indagar el daño en el material genético de una población infantil expuesta potencialmente a pesticidas en el ambiente. El diseño metodológico fue de tipo observacional y transversal. Participaron en el estudio 48 niños expuestos potencialmente a pesticidas y 46 niños no expuestos. Se obtuvo muestra de la mucosa bucal para determinar daño en el material genético a través de la frecuencia de micronúcleos (MN). Se encontró en el grupo expuesto potencialmente a pesticidas un promedio mayor de micronúcleos (5,1±2,9 vs 1,8±2,0; p<0,0001), un promedio mayor de células binucleadas, (3,5±2,7 vs 1,4±1,4; p<0,0001), mayor frecuencia de cariorrexis (18,2±18,4 vs 5,8±18,4; p<0,004) y picnosis (24,8± 18,0 vs 17,1±8,3; p<0,03). El 40% (19/47) de los niños expuestos potencialmente a pesticidas tuvieron un tiempo de exposición de 6 años. Esta investigación aporta evidencias de daño genético en la población expuesta potencialmente a pesticidas en el ambiente Palabras claves: Genotoxicidad- pesticidas -niños-medio ambiente.

Pesticides can cause acute or chronic effects on human health. Many pesticides can cause damage to genetic material. These changes in genetic information have been associated with an increased risk of cancer. The objective of this study is to investigate damage to genetic material in a population of children potentially exposed to pesticides in the environment. The study design was observational and cross sectional. We studied 48 children potentially exposed to pesticides and 46 unexposed children. Samples of the oral mucosa were obtained to determine damage to genetic material using micronucleus frequency (MN).Higher average micronucleus counts were found in the group potentially exposed to pesticides (5.1±2.9 vs. 1.8±2.0, p <0.0001), along with a higher average of binucleate cells (3.5±2.7 vs. 1.4±1.4 p <0.0001), higher frequency of karyorrhexis (18.2±18.4 vs. 5.8± 18.4, p <0.004) and pyknosis (24.8±18.0 vs. 17.1±8.3; p <0.03).Of the children potentially exposed to pesticides, 40% (19/47) had an exposure time of 6 years. This study provides evidence of genetic damage in the population potentially exposed to pesticides in the environment.

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PURPOSE: Renal angiomyolipoma (AML) is a benign tumor, corresponding to approximately 3 percent of solid renal tumors, which has in its composition endothelial cells, myocytes and adipocytes. One of the rare complications of this type of tumor is linfonodal involvement and tumor venous dissemination forming thrombus in the renal vein and inferior vena cava and may even reach the right atrium. Surgical treatment of this type of tumor is performed mainly by open surgery, but this video shows the laparoscopic approach for level III of angiomyolipoma, showing that this approach is feasible and reproducible by any trained and experienced surgeon. MATERIALS AND METHODS: Woman of 65 years with back pain, ultrasound examination showed a tumor in the right kidney, and follow-up computed tomography revealed aspects of angiomyolipoma. We performed radical nephrectomy with thrombectomy by laparoscopy. RESULTS: The patient recovered well, enjoying all the benefits of laparoscopic surgery. There were no complications during surgery. CONCLUSIONS: The surgical approach of laparoscopic renal angiomyolipoma is a perfectly feasible, bringing numerous benefits to the patient, and can establish itself as a breakthrough in treating this type of tumor.

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Los pesticidas pueden producir efectos agudos o crónicos en la salud humana. Muchos de ellos pueden provocar daño en el material genético. Esta modificación en la información genética se ha relacionado con un alto riesgo de padecer cáncer. El objetivo del presente trabajo es indagar el daño en el material genético de una población infantil expuesta potencialmente a pesticidas en el ambiente. El diseño metodológico fue de tipo observacional y transversal. Participaron en el estudio 48 niños expuestos potencialmente a pesticidas y 46 niños no expuestos. Se obtuvo muestra de la mucosa bucal para determinar daño en el material genético a través de la frecuencia de micronúcleos (MN). Se encontró en el grupo expuesto potencialmente a pesticidas un promedio mayor de micronúcleos (5,1±2,9 vs 1,8±2,0; p<0,0001), un promedio mayor de células binucleadas, (3,5±2,7 vs 1,4±1,4; p<0,0001), mayor frecuencia de cariorrexis (18,2±18,4 vs 5,8±18,4; p<0,004) y picnosis (24,8± 18,0 vs 17,1±8,3; p<0,03). El 40% (19/47) de los niños expuestos potencialmente a pesticidas tuvieron un tiempo de exposición de 6 años. Esta investigación aporta evidencias de daño genético en la población expuesta potencialmente a pesticidas en el ambiente.

Pesticides can cause acute or chronic effects on human health. Many pesticides can cause damage to genetic material. These changes in genetic information have been associated with an increased risk of cancer. The objective of this study is to investigate damage to genetic material in a population of children potentially exposed to pesticides in the environment. The study design was observational and cross sectional. We studied 48 children potentially exposed to pesticides and 46 unexposed children. Samples of the oral mucosa were obtained to determine damage to genetic material using micronucleus frequency (MN).Higher average micronucleus counts were found in the group potentially exposed to pesticides (5.1±2.9 vs. 1.8±2.0, p <0.0001), along with a higher average of binucleate cells (3.5±2.7 vs. 1.4±1.4 p <0.0001), higher frequency of karyorrhexis (18.2±18.4 vs. 5.8± 18.4, p <0.004) and pyknosis (24.8±18.0 vs. 17.1±8.3; p <0.03).Of the children potentially exposed to pesticides, 40% (19/47) had an exposure time of 6 years. This study provides evidence of genetic damage in the population potentially exposed to pesticides in the environment.

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We describe the relative frequency, clinical features, neuroimaging and pathological results, and outcome after pharmacological or surgical intervention for a series of pediatric patients with temporal lobe epilepsy (TLE) from an epilepsy center in Brazil. The medical records of children younger than 12 years with features strongly suggestive of TLE were reviewed from January 1999 to June 1999. Selected children were evaluated regarding clinical, EEG, and magnetic resonance imaging (MRI) investigation and divided into three groups according to MRI: group 1 (G1, N = 9), patients with hippocampal atrophy; group 2 (G2, N = 10), patients with normal MRI, and group 3 (G3, N = 12), patients with other specific temporal lesions. A review of 1732 records of children with epilepsy revealed 31 cases with TLE (relative frequency of 1.79 percent). However, when the investigation was narrowed to cases with intractable seizures that needed video-EEG monitoring (N = 68) or epilepsy surgery (N = 32), the relative frequency of TLE increased to 19.11 (13/68) and 31.25 percent (10/32), respectively. At the beginning of the study, 25 of 31 patients had a high seizure frequency (80.6 percent), which declined to 11 of 31 (35.5 percent) at the conclusion of the study, as a consequence of pharmacological and/or surgical therapy. This improvement in seizure control was significant in G1 (P < 0.05) and G3 (P < 0.01) mainly due to good postsurgical outcome, and was not significant in G2 (P > 0.1, McNemar's test). These results indicate that the relative frequency of TLE in children was low, but increased considerably among cases with pharmacoresistant seizures. Patients with specific lesions were likely to undergo surgery, with good postoperative outcomes.

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We describe the relative frequency, clinical features, neuroimaging and pathological results, and outcome after pharmacological or surgical intervention for a series of pediatric patients with temporal lobe epilepsy (TLE) from an epilepsy center in Brazil. The medical records of children younger than 12 years with features strongly suggestive of TLE were reviewed from January 1999 to June 1999. Selected children were evaluated regarding clinical, EEG, and magnetic resonance imaging (MRI) investigation and divided into three groups according to MRI: group 1 (G1, N = 9), patients with hippocampal atrophy; group 2 (G2, N = 10), patients with normal MRI, and group 3 (G3, N = 12), patients with other specific temporal lesions. A review of 1732 records of children with epilepsy revealed 31 cases with TLE (relative frequency of 1.79%). However, when the investigation was narrowed to cases with intractable seizures that needed video-EEG monitoring (N = 68) or epilepsy surgery (N = 32), the relative frequency of TLE increased to 19.11 (13/68) and 31.25% (10/32), respectively. At the beginning of the study, 25 of 31 patients had a high seizure frequency (80.6%), which declined to 11 of 31 (35.5%) at the conclusion of the study, as a consequence of pharmacological and/or surgical therapy. This improvement in seizure control was significant in G1 (P < 0.05) and G3 (P < 0.01) mainly due to good postsurgical outcome, and was not significant in G2 (P > 0.1, McNemar's test). These results indicate that the relative frequency of TLE in children was low, but increased considerably among cases with pharmacoresistant seizures. Patients with specific lesions were likely to undergo surgery, with good postoperative outcomes.

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Amostras de tecidos colhidas à necropsia de uma vaca da raça Holandesa, nove anos de idade, foram fixadas em formol neutro a 10% e enviadas para exames histológicos. A macroscopia não revelou lesões nodulares cutâneas típicas de demodicose. Secções microscópicas de tegumento cutâneo da vulva evidenciaram ácaros (Demodex bovis) isolados ou em grupos no interior de folículos pilosos e glândulas sebáceas.

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Amostras de tecidos colhidas à necropsia de uma vaca da raça Holandesa, nove anos de idade, foram fixadas em formol neutro a 10% e enviadas para exames histológicos. A macroscopia não revelou lesões nodulares cutâneas típicas de demodicose. Secções microscópicas de tegumento cutâneo da vulva evidenciaram ácaros (Demodex bovis) isolados ou em grupos no interior de folículos pilosos e glândulas sebáceas.(AU)

Tissues samples collected at necropsy from a 9 year-old Holstein cow were fixed in buffered 10% formalin for histological examination. Macroscopic finding did not reveal typical nodular cutaneous lesions. Vulvar microscopic sections of cutaneous tegument showed mites (Demodex bovis), alone and grouped in hair follicles and sebaceous glands.(AU)

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This work has evaluated the clastogenicity of six extracts (tea and aqueous extract of leaves, tea, aqueous and methanolic extracts of dried fruit, and tea of unripe fruit) obtained from Crotalaria retusa L. and three extracts (tea and methanolic extract of dried fruit, and tea of unripe fruit) obtained from Crotalaria mucronata Desv. The extracts were injected intraperitoneally into mice, and the animals were killed 24 h after treatment for preparation of bone marrow cells. The extracts obtained from fruits of Crotalaria retusa were found to cause a dose-dependent increase in the frequency of chromosomal aberrations in mice. On the other hand, no statistically significant increase in the frequency of aberrant cells was observed for the animals treated with leaf extracts obtained from Crotalaria retusa and with extracts from fruits of Crotalaria mucronata. The possibility that the pyrrolizidine alkaloid, monocrotaline, present in Crotalaria retusa exerts a clastogenic effect on mouse bone marrow cells is discussed. Our conclusion is based on studies using intraperitoneal treatments. Effects of oral exposure to extracts of Crotalaria retusa are unknown.

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Toxicological and toxicogenetic effects of aqueous (tea) and hexanic fruit extract of Indigofera suffruticosa Mill. and hydroalcoholic root extract of Solanum agrarium Stendt. were evaluated in Balb C male mice intraperitoneally exposed. A hepatotoxic effect was observed just for animals treated with aqueous fruit extract of I. suffruticosa. In relation to the toxicogenetic effect, just the group treated with 12.5% of toxic dose of aqueous fruit extract of I. suffruticosa showed a statistically significant increase in the frequency of cells with chromosome aberrations (cytogenetic effect), although a slight increase was also observed for the highest dose (25% of LD50) of hydroalcoholic root extract of S. agrarium. The results obtained show that before S. agrarium is used as medicine and before the wide use of I. suffruticosa in cattle food, careful evaluation must be done.

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Aflatoxinas säo um grupo de metabólitos tóxicos, produzidos principalmente pelos fungos Aspergillus flavus e Aspergillus parasiticus. No Brasil, estäo associados frequentemente a amendoim, algodäo, feijäo e milho e merecem atençäo por sua natureza carcinogênica. A aflatoxina B1 é a mais tóxica e está sempre presente em maior concentraçäo que G1, B2 e G2. O presente trabalho levanta a contaminaçäo de 25 amostras de gräos de milho coletadas em armazéns de pequenos produtores de regiäo de Irecê-BA(1985), por fungos aflatoxigênicos e aflatoxina B1, considerando que o produto é utilizado na alimentaçäo humana e animal. As amostras foram submetidas a exames fitopatológicos e químicos que revelaram a presença de cepas toxigênicas de A. parasiticus e A. flavus em 100% e 91,5% das amostras, respectivamente, e a ocorrência de aflatoxina B1 em níveis näo recomendados em 12% das amostras, o que as torna inadequadas para consumo

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A leguminosa indigofera suffruticosa Mill, apresenta potencial forrageiro. Folhas e Frutos foram extraídos com hexano e os extratos obtidos, administrados por via intraperitoneal em camundongos. Encontrou-se a dose tóxica de 5,0g/kg e 7,5g/kg para os extratos hexânicos de folha e fruto respectivamente. As principais alteraçöes foram observadas a nível hepático e pulmonar

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