RESUMEN
BACKGROUND: The herpes virus enters into latency after symptomatic or asymptomatic herpetic infection. During latency, the virus has no impact on infected cells. However, internal or external stimuli, including certain lasers, can induce virus reactivation. OBJECTIVE: The aim was to study the reactivation power of the low-intensity diode and CO(2) lasers on the latent herpes virus. MATERIALS AND METHODS: The bovine herpesvirus 1 (BHV-1) was inoculated in either the nasal cavity or the lacrimal film of an animal model. Once the virus entered into latency, the trigeminal ganglia of animals were exposed to either a low-intensity diode or CO(2) laser. The reactivation of the virus was then explored by PCR, RT-PCR, and dot-blot hybridization on nasal or lacrimal swabs. The accuracy, sensitivity, and specificity of the aforementioned techniques were compared. RESULTS: The low-intensity diode laser reactivated the herpes virus less than the CO(2) laser. The nasally inoculated virus was more liable for reactivation by both lasers. PCR was considered as the standard method for the detection of the reactivated virus. CONCLUSIONS: Low-intensity diode and CO(2) lasers can induce herpes virus reactivation, with the diode laser less likely to reactivate the virus than the CO(2) laser.
Asunto(s)
Herpesviridae/fisiología , Láseres de Gas/efectos adversos , Latencia del Virus , Animales , Modelos Animales de Enfermedad , Electroforesis en Gel de Agar , Conejos , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
OBJECTIVE: The aim of this study was to evaluate the diode laser in excision biopsy of superficial proliferative tongue lesions under local anesthesia. BACKGROUND DATA: Most mobile tongue lesions present as surface projections and have similar clinical features. They are usually excised for diagnostic purposes using conventional surgery or electrosurgery. The diode laser has proven itself useful in different oral surgeries. METHODS: We report the results of a prospective pilot study where contact diode laser was used in excision biopsy of six mobile tongue lesions. All excised tissues were pathologically examined. To assess post-operative pain, patients were asked to correlate it to an 11-point Visual Analogue Scale (VAS); patients were also asked to assess their impression of alteration in tongue size on a 4-point scale. Healing, functional results, and recurrence rate were assessed. RESULTS: All interventions were uneventful and done at the same out-patient setting. All lesions were benign. Healing occurred by secondary intention in the six cases with no residual ulceration. Postoperative pain was mild in four cases, while two cases suffered from moderate pain. Post-operative impression of tongue swelling occurred in all cases. The mild impression disappeared by the end of the first week and the moderate one by the end of the second week. No recurrence was observed over a mean follow-up period of 15 months. CONCLUSION: The diode 980-nm laser is optimal for excision biopsy of superficial and benign proliferative lesions of the mobile tongue.
Asunto(s)
Terapia por Láser , Enfermedades de la Lengua/cirugía , Adulto , Biopsia/métodos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto , Estudios Prospectivos , Enfermedades de la Lengua/patologíaRESUMEN
Laryngeal chondrosarcoma is a rare tumor that is known for its indolent course and its tendency to ultimate recurrence. According to the actually adopted classification, 95% of the reported cases are of a low-grade type. A consensus recognizing conservative surgery as the most reasonable treatment for these lesions has almost been reached. However, fear of jeopardizing the patency of the laryngeal airway as a result of a wide cricoid excision and also the fear of repeated recurrences could still push some surgeons to perform a total laryngectomy in the case of laryngeal chondrosarcoma. After a brief review of the literature, we will present five cases of laryngeal chondrosarcoma that were treated and followed at the Clermont-Ferrand University Medical Center over the last two decades. These cases exhibit many of the clinicopathologic features of the tumor and illustrate the pitfalls of diagnosis and treatment. In light of this presentation, we will discuss the widely accepted management as well as a newly suggested treatment modality for this disease.