RESUMEN
Blood serum samples collected from patients with acute hepatitis symptoms admitted to Infectious Disease Hospitals of Novosibirsk, Barnaul, and Irkutsk were studied. The serum samples were tested for the IgM and IgG antibodies to HEV using ELISA. Seropositive samples were tested using RT-PCR for HEV RNA. Two HEV strains were isolated, and thus HEV infection was identified for West Siberia. One of this strains is classified as HEV genotype I; the other, as genotype III. Cell culturing of these strains in green monkey kidney (4647) cells showed an ability of HEV genotype I strain to cause persistent infection.
Asunto(s)
Virus de la Hepatitis E/metabolismo , Hepatitis E/metabolismo , Replicación Viral/fisiología , Animales , Línea Celular , Chlorocebus aethiops , Genotipo , Anticuerpos Antihepatitis/sangre , Hepatitis B/sangre , Hepatitis B/complicaciones , Hepatitis B/genética , Hepatitis B/metabolismo , Hepatitis E/sangre , Hepatitis E/complicaciones , Hepatitis E/genética , Virus de la Hepatitis E/genética , Virus de la Hepatitis E/aislamiento & purificación , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Filogenia , ARN Viral/sangre , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Siberia , Cultivo de VirusRESUMEN
Comparative study of replication markers of hepatitis C virus in various biological substrates and evaluation of activity of chronic HCV infection in liver biopsy specimens showed that replication of hepatitis C virus and the number of HCV-infected hepatocytes did not promote liver damage in chronic hepatitis C. These findings indicate that the expression of antiviral reactions by liver parenchyma cells plays the key role in the morphogenesis of HCV infection.
Asunto(s)
Hepacivirus/fisiología , Hígado/patología , Replicación Viral , Adolescente , Adulto , Anciano , Femenino , Hepacivirus/genética , Hepatitis C/patología , Humanos , Hígado/ultraestructura , Hígado/virología , Masculino , Persona de Mediana Edad , Reacción en Cadena de la PolimerasaRESUMEN
We carried out a comparative study of replication markers of hepatitis C virus (HCV RNA) in some biological substrates and NS3-antigen in liver biopsy specimens. It was found that 82% liver specimens contained RNA HCV, in 44% cases HCV RNA was present in the serum, mononuclear blood cells, and liver. The presence of NS3-antigen in hepatocytes can be considered as a structural marker of HCV replication, which is confirmed by positive correlation with the results of PCR for viral RNA in tissue specimens. In most cases the presence of HCV replication markers did not correlate with activity of the infectious process assessed by biochemical and histological tests.
Asunto(s)
Hepacivirus/genética , Hepatitis C/patología , Hepatitis C/virología , Hígado/patología , Adolescente , Adulto , Biopsia , Enfermedad Crónica , ADN Viral/análisis , Femenino , Hepatocitos/metabolismo , Hepatocitos/virología , Humanos , Inmunohistoquímica , Metabolismo de los Lípidos , Hígado/metabolismo , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , ARN Viral/análisisRESUMEN
Study of the prevalence of hepatitis C virus (HCV) markers in 153 patients of Municipal Infectious Diseases Clinical Hospital No. 1 in Novosibirsk revealed anti-HCV in 88.2% patients and viral RNA in 69.3% samples. For 93 Siberian HCV isolates 5'-UTR regions were amplified and sequenced. Comparison of these nucleotide sequences with databank showed that 63.4% HCV isolates belonged to subtype 1b, 7.5% to genotype 2, and 18.3% to genotype 3. In the rest HCV isolates the 5'-UTR sequences contained heretofore undescribed nucleotide substitutions, insertions, or deletions.