RESUMEN
The aim of the work was to investigate the phospholipid composition and parameters of platelet aggregation in patients with hypo- and hyperthyroidism. The data obtained have shown that phospholipid liposomes added exogenously, changed the function of cell hemostasis and, in particular, decreased platelet aggregation. Unlike the other phospholipids, phosphatidylinositol, cardiolipin and phosphatidylserine produced the most significant effect on this index and corrected, to a certain extent, disturbed platelet functions in hypothyroidism.
Asunto(s)
Plaquetas/metabolismo , Hipertiroidismo/sangre , Hipotiroidismo/sangre , Lípidos/sangre , Agregación Plaquetaria/fisiología , Adenosina Difosfato , Cromatografía en Capa Delgada , Humanos , Fosfolípidos/sangre , Agregación Plaquetaria/efectos de los fármacos , Inhibidores de Agregación PlaquetariaRESUMEN
The causes of insulin resistance were studied in rats at late terms of pregnancy. A decrease in the sensitivity to insulin dependent incorporation of glucose in total lipids and CO2 of isolated fatty tissue of pregnant rats was shown. The maximum tissue reaction to insulin (reactivity) did not lessen indicating together with hyperinsulinemia after a glucose tolerance test and a rise of the activity of blood serum insulin-like growth factors the absence of postreceptor defects. An increase in the pregnant rats blood levels of cholesterol, triglycerides and lipoprotein atherogenic fractions was revealed. The latter in physiological concentrations made an inhibitory effect on respiration of liver and thyroid sections of intact rats. Together with the action of hormones of the fetoplacental system it was considered as one of the mechanisms of development of insulin resistance at late terms of pregnancy.
Asunto(s)
Resistencia a la Insulina , Preñez/efectos de los fármacos , Animales , Glucemia/análisis , Corticosterona/sangre , Femenino , Glucosa/administración & dosificación , Prueba de Tolerancia a la Glucosa , Insulina/administración & dosificación , Insulina/sangre , Lípidos/sangre , Lipoproteínas/sangre , Ovariectomía , Embarazo , Preñez/sangre , Ratas , Somatomedinas/sangre , Factores de TiempoRESUMEN
Correlations between change in the composition of thyroid nodal tissue individual phospholipids and intensity of oxidizing processes under the influence of various phospholipid liposomes were studied. The thyroid phospholipid fraction level was investigated in patients with nodular goiter. A decrease in the level of sphingomyelin and phosphatidylinositide by 54%, cardiolipin by 63%, phosphatidic acid by 70%, phosphatidylserine by 46%, phosphatidylcholine by 7% and a two-fold increase in the level of phosphatidylethanolamine as compared to the control one were shown. A study of a degree of reduction of oxidation metabolism by adding phospholipid liposomes to thyroid sections showed that the tissue of a node possessed weaker oxidizing activity as compared to the control one. O2 absorption and 14CO2 excretion up to 30% were lowered in a hyperthyroid node. An activating liposome effect was noted in thyroid nodal tissue, mostly in the presence of phosphatidylserine, cardiolipin and phosphatidylinositide; the weakest effect was produced by phosphatidylethanolamine and phosphatidylserine.
Asunto(s)
Bocio Nodular/metabolismo , Hipotiroidismo/metabolismo , Fosfolípidos/metabolismo , Glándula Tiroides/metabolismo , Transporte Biológico , Glucosa/metabolismo , Bocio Nodular/complicaciones , Humanos , Hipotiroidismo/complicaciones , Yodo/metabolismo , Oxidación-Reducción , Fosfolípidos/fisiologíaRESUMEN
It was shown that 7 to 10 days' injection of lipoproteins (LPs), isolated from the human blood serum according to Burstein and Scholnick method, induces significant inhibition of the rat thyroid iodine-absorbing function. Alpha-LPs exert more evident inhibiting effect than beta + pre- beta-LPs, and beta + pre- beta-LPs from the oncological patients' blood serum have more pronounced inhibiting effect comparatively to the same fraction of the donors; blood serum. 10-days' LP injection is accompanied by thyroxine inhibition and iodothyrozine/iodothyronine ratio rise in the thyroid tissue. In the experiments in vitro and in vivo several Lp fractions decreased thyrotropin-stimulating effect on AMP accumulation in the rat thyroid gland. Possible mechanisms of LP action on the thyroid function are discussed. The study of LP effect on the thyroid function in homologous system is necessary. It is suggested that hyperlipidemia (hyperlipoproteinemia) is one of the factors, contributing to the thyroid function decrease with age and in patients with malignant tumors.
Asunto(s)
Lipoproteínas/farmacología , Glándula Tiroides/efectos de los fármacos , Absorción , Animales , AMP Cíclico/biosíntesis , Depresión Química , Humanos , Técnicas In Vitro , Yodo/metabolismo , Lipoproteínas/sangre , Masculino , Neoplasias/sangre , Ratas , Glándula Tiroides/metabolismoRESUMEN
The functional activity of the thyroid glands of the pregnant rats and their foetuses prior to the birth and the deiodizing ability of the liver of the mother and the foetus on the 18-th day of pregnancy were studied. The reliable differences were shown between the levels of protein bound iodine in the blood serum of the pregnant rats as compared with the control ones, as well as the high content of thyroid hormones in the blood serum of the foetuses. The process of thyroxin and diiodothyrosine deiodizing in the foetal liver was more active than in the mother. The results obtained suggest the increase of functional activity of the thyroid gland in the mother by the end of pregnancy and the presence of such an activity in the foetus, as well as the high activity of the deiodizing system in the foetal liver during the last days of prenatal development.
Asunto(s)
Feto/fisiología , Glándula Tiroides/fisiología , Hormonas Tiroideas/metabolismo , Animales , Proteínas Sanguíneas/metabolismo , Diyodotirosina/metabolismo , Femenino , Edad Gestacional , Yodo/sangre , Hígado/metabolismo , Intercambio Materno-Fetal , Embarazo , Unión Proteica , Ratas , Tiroxina/metabolismoRESUMEN
This is an experimental work. Investigations were carried out on albino rats on the 18th day of pregnancy. A study was made of I-131-labelled thyroxin penetration from the maternal organism into the fetus and vice versa. Radiological and radiochromatographic analysis of the blood samples of both the mother and the fetuses at various time intervals after the administration of radioactive thyroxin showed that the isotope in the form of thyroxin failed to penetrate from the mother to the fetus and vice versa. A conclusion was drawn that during prenatal development the function of the fetal thyroid gland was automatic.