RESUMEN
INTRODUCTION: Mentha arvensis L. is the most valuable medicinal plant that possesses anti-inflammatory, hepatoprotective, antimicrobial, and antioxidant properties. There are few studies available in the literature about M. arvensis L nanoparticles, but their nanosuspensions-based information remains unclear and needs further study. OBJECTIVE: This study was designed to explore the nanotechnology approach for biochemical characterisation, enhanced bioactivities, and photochemistry of freshly prepared M. arvensis L. nanosuspensions. METHODOLOGY: Nanosuspensions of M. arvensis L. leaves were prepared by following the nanoprecipitation method. In this study, we performed structural and biochemical characterisation through analyses of Fourier-transform infrared (FTIR) spectroscopy, high-performance liquid chromatography (HPLC), phase contrast microscopy and enhanced bioactivities; antioxidant, alpha-amylase inhibition, glycation inhibition and cytotoxicity assays. RESULTS: FTIR analysis revealed the presence of phenols, amines hydroxyl, carboxylic acid, alkenes, alkenes and alkynes. HPLC analysis revealed the presence of chlorogenic acid, a principal phenolic component. Biofilm inhibition activity revealed that the growth formation of Escherichia coli inhibited up to 62.4% and 53.35% by leaves extract and nanosuspension, respectively. However, the growth of Staphylococcus aureus was not inhibited by nanosuspension and extract. Nanosuspension and extract exhibited 155.92 mg, 108.11 mg gallic acids per 100 g of dry weight total phenolic content and 233.44 mg, 163.933 mg catechin per 100 g of dried weight total flavonoid content in extract and nanosuspension, respectively. Antioxidant activity revealed the scavenging potential of nanosuspensions and extract was 41.01% and 12.07%, respectively. Alpha-amylase inhibiting activity of nanosuspension and extract was 36% and 33%, while, the antiglycation potential of nanosuspension and extract were 41.68% and 35.18%, respectively. Nanosuspensions and extract showed maximum hemolytic activity at 12.91% and 17.18%, respectively. CONCLUSION: These cost-effective nanoformulations could serve as a platform for therapeutic purposes in controlling the high risk of infectious diseases and designing efficient plant nanosuspensions by discovering novel bioactive compounds in an adequate manner.