RESUMEN

CONTEXT: Clinical study of breast cancer patients in Chicago, IL, USA. OBJECTIVE: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. METHODS: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. RESULTS: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. CONCLUSIONS: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density.

Asunto(s)

Neoplasias de la Mama/genética , Roturas del ADN de Cadena Simple , Estrógenos/análisis , Adulto , Densidad de la Mama , Neoplasias de la Mama/diagnóstico , Daño del ADN , Enzimas Reparadoras del ADN/análisis , Estrógenos de Catecol/análisis , Femenino , Humanos , Persona de Mediana Edad , Riesgo