RESUMEN
Fumonisin B1 (FB1) is a carcinogenic mycotoxin produced by Fusarium species contaminating maize. At present, fumonisin determination is performed using costly and demanding chromatography techniques or immunoassays. Recently, a molecularly imprinted polymer nanoparticles (nanoMIPs) - based assay (MINA) has been developed for FB1 detection. Herein, we have applied MINA for the determination of FB1 in naturally contaminated maize samples and results were compared with those obtained with ELISA and a reference HPLC method (AOAC No. 2001.04). The nanoMIPs as a recognition element mimicking antibodies used in ELISA were produced by solid phase synthesis and used in MINA for FB1 determination in 53 maize samples. As a result, 18 maize samples were contaminated with FB1 at levels higher than 0.25â¯mg/kg. Fumonisin concentrations from samples measured by MINA were well correlated with those using ELISA and HPLC. Therefore, MINA could be used as an alternative technique for FB1 determination in maize.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática/métodos , Fumonisinas/análisis , Impresión Molecular , Nanopartículas/química , Polímeros/química , Zea mays/química , Cromatografía Líquida de Alta Presión , Zea mays/metabolismoRESUMEN
The influence of lyophilisation, autoclaving and sonication on the stability and performance of trypsin-specific molecularly imprinted polymer nanoparticles (MIP NPs) has been studied in order to improve their long-term physical stability. Glucose, glycine, sorbitol and trehalose were tested as cryoprotectant agents during the lyophilisation treatment. The effect of lyophilisation and sterilisation on affinity of trypsin-specific NPs was assessed using Biacore 3000 instrument. The results have demonstrated that MIP NPs successfully withstood the lyophilisation and autoclaving conditions without a reduction of their recognition properties and affinity. It is possible to conclude that both tested lyophilisation and sterilisation treatments were suitable for a long-term storage of the prepared MIP NPs and could be used to store MIP NPs in dry state and hence reduce the chance of the bacterial contamination. An effective preservation of the MIP NPs is a crucial requirement for their future applications in the clinical diagnostics and bioimaging.