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Rev Argent Microbiol ; 48(2): 122-7, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27291283

RESUMEN

Blinded rechecking is a method proposed for external quality assurance (EQA) of auramine-stained acid-fast bacilli (AFB) smears using fluorescence microscopy (FM), however, this procedure is not well developed and slides fading over time could compromise its implementation. Since bleaching of fluorescent molecules involves temperature-dependent chemical reactions, it is likely that low temperatures could slow down this process. We stored auramine-stained slides under different environmental conditions, including -20°C, and examined them over time. The slides stored in all the environments faded. At -20°C, fading was not reduced in relation to room temperature. Restaining and re-examining smears after five months showed that the slides containing saliva and storage at -20°C were associated with failure in AFB reappearance. In conclusion, the practice of freezing slides until they are viewed should be discouraged as it has a negative effect on blinded rechecking by reducing reading concordance after restaining. Specimen quality should be considered when interpreting FM-EQA results.


Asunto(s)
Benzofenoneido/efectos de la radiación , Colorantes Fluorescentes/efectos de la radiación , Microscopía Fluorescente/métodos , Fotoblanqueo , Garantía de la Calidad de Atención de Salud/métodos , Esputo/microbiología , Coloración y Etiquetado/métodos , Tuberculosis/diagnóstico , Argentina , Benzofenoneido/análisis , Criopreservación , Estudios de Factibilidad , Colorantes Fluorescentes/análisis , Humanos , Iluminación , Microscopía Fluorescente/instrumentación , Variaciones Dependientes del Observador , Valor Predictivo de las Pruebas , Preservación Biológica/métodos , Reproducibilidad de los Resultados , Método Simple Ciego , Temperatura
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