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Can J Vet Res ; 69(2): 143-5, 2005 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-15971679

RESUMEN

Spoligotyping was applied to 44 isolates of Mycobacterium bovis obtained from the Canadian province of Manitoba. Isolates were obtained from submissions of elk (n = 16), deer (n = 1), and cattle (n = 27) tissues spanning the period of 1990 to early 2003. Two spoligotype profiles were obtained differing only in the reaction with oligonucleotide number 12. Forty of the 44 isolates (90.9%) hybridized with oligonucleotide 12 (MB-1 type), while the remaining 4 of 44 (9.1%) did not show a signal at position 12 (MB-2 type). Octal codes for these 2 types are 656573377603600 and 656473377603600, respectively. These spoligotypes have not been reported as occurring elsewhere worldwide.


Asunto(s)
Técnicas de Tipificación Bacteriana/veterinaria , Ciervos/microbiología , Mycobacterium bovis/clasificación , Tuberculosis Bovina/microbiología , Tuberculosis/veterinaria , Animales , Técnicas de Tipificación Bacteriana/métodos , Bovinos , ADN Bacteriano/análisis , Manitoba , Oligonucleótidos/análisis , Tuberculosis/microbiología
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