RESUMEN
Genome instability relies on preserving the chromatin structure, with any histone imbalances threating DNA integrity. Histone synthesis occurs in the cytoplasm, followed by a maturation process before their nuclear translocation. This maturation involves protein folding and the establishment of post-translational modifications. Disruptions in this pathway hinder chromatin assembly and contribute to genome instability. JMJD1B, a histone demethylase, not only regulates gene expression but also ensures a proper supply of histones H3 and H4 for the chromatin assembly. Reduced JMJD1B levels lead to the cytoplasmic accumulation of histones, causing defects in the chromatin assembly and resulting in DNA damage. To investigate the role of JMJD1B in regulating genome stability and the malignancy of melanoma tumors, we used a JMJD1B/KDM3B knockout in B16F10 mouse melanoma cells to perform tumorigenic and genome instability assays. Additionally, we analyzed the transcriptomic data of human cutaneous melanoma tumors. Our results show the enhanced tumorigenic properties of JMJD1B knockout melanoma cells both in vitro and in vivo. The γH2AX staining, Micrococcal Nuclease sensitivity, and comet assays demonstrated increased DNA damage and genome instability. The JMJD1B expression in human melanoma tumors correlates with a lower mutational burden and fewer oncogenic driver mutations. Our findings highlight JMJD1B's role in maintaining genome integrity by ensuring a proper histone supply to the nucleus, expanding its function beyond gene expression regulation. JMJD1B emerges as a crucial player in preserving genome stability and the development of melanoma, with a potential role as a safeguard against oncogenic mutations.
Asunto(s)
Daño del ADN , Inestabilidad Genómica , Histonas , Histona Demetilasas con Dominio de Jumonji , Melanoma , Neoplasias Cutáneas , Animales , Humanos , Ratones , Línea Celular Tumoral , Daño del ADN/genética , Regulación Neoplásica de la Expresión Génica , Histonas/metabolismo , Histona Demetilasas con Dominio de Jumonji/metabolismo , Histona Demetilasas con Dominio de Jumonji/genética , Melanoma/genética , Melanoma/patología , Melanoma/metabolismo , Melanoma Experimental/genética , Melanoma Experimental/patología , Melanoma Experimental/metabolismo , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/patología , Neoplasias Cutáneas/metabolismoRESUMEN
Mexico is an endemic region for dengue virus (DENV). The increase in this disease coincides with outbreaks of COVID-19, both of which are single-stranded positive RNA viruses. These characteristics make it difficult to distinguish each disease because they share clinical and laboratory features, which can consequently result in misdiagnoses. This is why the use of precision confirmatory tests (qRT-PCR) are crucial for early diagnosis. We herein report a pediatric patient who presented a coinfection for DENV and COVID-19, "SARS-CoV-2/Dengue". This patient initially presented a fever, cough, and headache and, three days later, developed generalized pain and epistaxis. Blood studies revealed thrombocytopenia and leukopenia, and the patient was admitted to the hospital for a probable DENV infection. Within 48 h, qRT-PCR tests specific for SARS-CoV-2 and DENV were performed and resulted as positive. The patient immediately received pharmacological treatment with azithromycin, oseltamivir, and metamizole. During hospitalization (9 days), the patient had no signs of respiratory distress and maintained normal body temperature and normal blood oxygen saturation. This case warns of the need for early diagnosis and adequate clinical and pharmacological management in the face of a "SARS-CoV-2/Dengue" coinfection. Early molecular detection of both viruses and timely treatment helped the patient to achieve a favorable recovery.
RESUMEN
Although there are several pathways to ensure that proteins are folded properly in the cell, little is known about the molecular mechanisms regulating histone folding and proteostasis. In this work, we identified that chaperone-mediated autophagy (CMA) is the main pathway involved in the degradation of newly synthesized histones H3 and H4. This degradation is finely regulated by the interplay between HSC70 and tNASP, two histone interacting proteins. tNASP stabilizes histone H3 levels by blocking the direct transport of histone H3 into lysosomes. We further demonstrate that CMA degrades unfolded histone H3. Thus, we reveal that CMA is the main degradation pathway involved in the quality control of histone biogenesis, evidencing an additional mechanism in the intricate network of histone cellular proteostasis.
Asunto(s)
Autofagia Mediada por Chaperones , Histonas , Autofagia , Histonas/metabolismo , Lisosomas/metabolismo , Biosíntesis de ProteínasRESUMEN
There is a lack of commercially available low-cost technologies to assess gait clinically in non-controlled environments. As a consequence of this, there has been poor massification of motion measurement technologies that are both objective and reliable in nature. Advances about the study of gait and its interpretation in recent years using inertial sensors have allowed proposing acceptable alternatives for the development of portable and low-cost systems that contribute to people's health in places and institutions that cannot acquire or maintain the operation of commercially available systems. A system based on a custom single Inertial Measurement Unit and a mobile application is proposed. Thus, an investigation is carried out using methodologies and algorithms found in the literature in order to get the main gait events and the spatial-temporal gait parameters. Twenty healthy Chilean subjects were assessed using a motion capture system simultaneously with the proposed tool. The results show that it is possible to estimate temporal gait parameters with slight differences respect gold--standard. We reach maximum mean differences of -2.35±5.02[step/min] for cadence, 0.03±0.04[sec] for stride time,0.02±0.03[sec] for step time, ±0.02[sec] for a single support time, 0.01±0.02[sec] for double support time and 0.01±0.03[m] for step length. As a result of experimental findings, we propose a new technological tool that can perform gait analysis. Our proposed system is user-friendly, low-cost, and portable. Therefore, we suggest that it could be an attractive technological tool that healthcare professionals could harness to objectively measure gait in environments that are either within the community or controlled. We also suggest that the tool could be used in countries where advanced clinical tools cannot be acquired. Therefore, we propose in this paper that our system is an attractive, alternative system that can be used for gait analysis by health professionals worldwide.
RESUMEN
BACKGROUND: Maintaining a proper supply of soluble histones throughout the cell cycle is important to ensure chromatin and genome stability. Following their synthesis, histones undergo a series of maturation steps to prepare them for deposition onto chromatin. RESULTS: Here, we identify the lysine demethylase JMJD1B as a novel player in the maturation cascade that contributes to regulate histone provision. We find that depletion of JMJD1B increases the protein levels of the histone chaperone tNASP leading to an accumulation of newly synthesized histones H3 and H4 at early steps of the histone maturation cascade, which perturbs chromatin assembly. Furthermore, we find a high rate of JMJD1B mutations in cancer patients, and a correlation with genomic instability. CONCLUSIONS: Our data support a role for JMJD1B in fine-tuning histone supply to maintain genome integrity, opening novel avenues for cancer therapeutics.
Asunto(s)
Inestabilidad Genómica , Histonas/metabolismo , Histona Demetilasas con Dominio de Jumonji/metabolismo , Procesamiento Proteico-Postraduccional , Células HeLa , Código de Histonas , Humanos , Histona Demetilasas con Dominio de Jumonji/genética , MutaciónRESUMEN
Resumo Objetivo comparar os efeitos do treinamento resistido (TR) e Pilates na qualidade de vida (QV) de idosas. Foi realizado um estudo de delineamento experimental com intervenção de 12 semanas. Métodos Foram avaliadas mulheres idosas (≥60 anos) que não praticavam nenhum tipo de exercício físico regularmente há pelo menos 3 meses. Participaram do estudo 41 idosas que foram aleatoriamente divididas em três grupos:1- grupo Pilates (GP), 2- grupo treinamento resistido (GTR) e 3- grupo controle (GC). Para avaliar a QV, foram utilizados os questionários WHOQOL-OLD, WHOQOL-BREF e SF-36. Foi utilizado o teste de Wilcoxon-Mann-Whitney para analisar a evolução das variáveis intragrupo e o teste de Kruskal-Wallis com post hoc de Dunn para as análises intergrupos. Para a comparação das variáveis de caracterização da amostra entre os grupos foi utilizado o teste qui-quadrado. Resultados Observou-se melhora do domínio Participação Social para o GP (p=0,016). No GTR, foi encontrada diferença significativa para o domínio Saúde Mental (p=0,019). No GC, observou-se melhora do domínio Participação Social (p=0,044) e Escore Total do WHOQOL-OLD (p=0,044). Nas comparações intergrupos, observou-se diferença do Escore Total do WHOQOL-BREF do GP e GTR, com o GP apresentando uma melhor QV no início do estudo (p=0,039). A Vitalidade (p=0,010) e Saúde Mental (p=0,024) do GTR melhorou em relação ao GP. Conclusão Os grupos analisados melhoraram sua QV após o período de intervenção.
Abstract Objective The objective of the study was to compare the effects of resistance training (RT) and Pilates on the quality of life (QOL) of elderly women. An experimental design study was carried out with a 12-week intervention. Methods Elderly women (≥60 years) who did not practice any type of physical exercise regularly for at least 3 months were evaluated. The study included 41 elderly women who were randomly divided into three groups: 1- Pilates group (PG), 2- Resistance training group (RTG) and 3- Control group (CG). To assess QOL, the WHOQOL-OLD, WHOQOL-BREF and SF-36 questionnaires were used. The Wilcoxon-Mann-Whitney Test was used to analyze the evolution of intragroup variables and the Kruskal-Wallis test with Dunn's post hoc for intergroup analyzes. To compare the sample characterization variables between the groups, the chi-square test was used. Results There was an improvement in the Social Participation domain for the PG (p=0.016). In the RTG, a significant difference was found for the Mental Health domain (p=0,019). In the CG, there was an improvement in the Social Participation domain (p=0.044) and Total WHOQOL-OLD Score (p=0.044). In the intergroup comparisons, there was a difference in the WHOQOL-BREF Total Score of the PG and RTG, with the PG presenting a better QOL at the beginning of the study (p=0.039). The Vitality (p=0,010) and Mental Health (p=0,024) of the RTG improved in relation to the PG. Conclusion The groups analyzed improved their QOL after the intervention period.
RESUMEN
Falls represent a major public health problem in the elderly population. The Timed Up & Go test (TU & Go) is the most used tool to measure this risk of falling, which offers a unique parameter in seconds that represents the dynamic balance. However, it is not determined in which activity the subject presents greater difficulties. For this, a feature-based segmentation method using a single wireless Inertial Measurement Unit (IMU) is proposed in order to analyze data of the inertial sensors to provide a complete report on risks of falls. Twenty-five young subjects and 12 older adults were measured to validate the method proposed with an IMU in the back and with video recording. The measurement system showed similar data compared to the conventional test video recorded, with a Pearson correlation coefficient of 0.9884 and a mean error of 0.17 ± 0.13 s for young subjects, as well as a correlation coefficient of 0.9878 and a mean error of 0.2 ± 0.22 s for older adults. Our methodology allows for identifying all the TU & Go subtasks with a single IMU automatically providing information about variables such as: duration of subâ»tasks, standing and sitting accelerations, rotation velocity of turning, number of steps during walking and turns, and the inclination degrees of the trunk during standing and sitting.
RESUMEN
ABSTRACT The objective of this review was to analyze the evidence of Pilates in physical fitness related to health in the elderly. The article indexing databases (Pubmed, Medline, Science Direct, Scielo, Lilacs, and Cochrane) were reviewed along with the Pilates, elderly and aging descriptors. A total of 41 studies with a randomized experimental and quasi-experimental design met the inclusion criteria. The selection of the studies was carried out by two researchers and the quality of the articles was assessed using the PEDro scale. Interventions ranged from 4-24 weeks with 1-3 sessions/week, and balance was the most investigated variable. The studies included in this review indicate that Pilates improves health status in the elderly, promoting gains in balance, muscle strength, flexibility, functional autonomy, muscular endurance, body composition and aerobic endurance. Despite these findings, some variables need to be further investigated. Level of Evidence II; Systematic review.
RESUMO O objetivo desta revisão foi analisar as evidências do Pilates na aptidão física relacionada com a saúde de idosos. Foram revisadas as bases de indexação de artigos (Pubmed, Medline, Science Direct, Scielo, Lilacs e Cochrane), com os descritores "Método pilates, idosos e envelhecimento". Atenderam os critérios de inclusão 41 estudos com delineamento randomizado experimental e "quase experimental". A seleção dos estudos foi realizada por dois pesquisadores e a qualidade dos artigos foi avaliada pela escala PEDro. As intervenções variaram entre 4 e 24 semanas, de 1 a 3 sessões/semana e a variável mais investigada foi o equilíbrio. Os estudos incluídos nesta revisão indicam que a prática de Pilates melhora as condições de saúde dos idosos, promovendo ganhos de equilíbrio, força muscular, flexibilidade, autonomia funcional, resistência muscular, composição corporal e resistência aeróbica. Apesar destes achados, algumas variáveis precisam ser mais investigadas. Nível de Evidência II; Revisão sistemática.
RESUMEN El objetivo de esta revisión fue analizar las evidencias del Pilates en la aptitud física relacionada a la salud de individuos de la tercera edad. Se revisaron las bases indexadoras de artículos (Pubmed, Medline, Science Direct, Scielo, Lilacs, y Cochrane), con los descriptores "Método pilates, ancianos y envejecimiento". Se cumplieron los criterios de inclusión 41 estudios con delineación experimental aleatorizada y "casi experimental". La selección de los estudios fue realizada por dos investigadores y la calidad de los artículos fue evaluada a través de la escala PEDro. Las intervenciones variaron entre 4-24 semanas, de 1-3 sesiones/semana y la variable más investigada fue el equilibrio. Los estudios incluidos en esta revisión indican que la práctica del Pilates mejora las condiciones de salud de los individuos de la tercera edad, promoviendo aumentos de equilibrio, fuerza muscular, flexibilidad, autonomía funcional, resistencia muscular, composición corporal y resistencia aeróbica. A pesar de estos hallazgos, algunas variables necesitan ser más investigadas. Nível de Evidencia II; Revision sistemática.
RESUMEN
The study of histone variants and histone posttranslational modifications (PTMs) is a trending topic in different fields including developmental biology, neurobiology, and immunology; as well as in the understanding of molecular mechanisms leading to diverse diseases, such as cancer. Since the establishment of histone PTMs starts immediately after their synthesis and it continues once they are assembled into chromatin, here we describe a classic protocol of subcellular fractionation aiming to study histones at different stages of maturation. This includes newly synthesized histones enriched in cytosolic fractions; a pool of newly synthesized, evicted, and stored histones enriched in nuclear soluble fractions; and chromatin-associated histones enriched in chromatin pellet. To study specific histone variants and the establishment of their PTMs, we describe a protocol for obtaining histone variants expressed in bacteria. In addition, we describe a Triton-Acetic acid-Urea (TAU) gel electrophoresis protocol adapted to work on mini-gels, which can be coupled to Western blot to analyze PTMs on histone variants. Finally, we describe a Chromatin immunoprecipitation (ChIP) assay for studying histone PTMs, or tagged histone variants, on specific DNA sequences.
Asunto(s)
Histonas/metabolismo , Biología Molecular/métodos , Procesamiento Proteico-Postraduccional , ADN/aislamiento & purificación , Células HeLa , Histonas/aislamiento & purificación , Humanos , Isoformas de Proteínas/metabolismo , Proteínas Recombinantes/metabolismo , Fracciones Subcelulares/metabolismoRESUMEN
This study compared training load and ratings of perceived exertion (RPE) during resistance training (RT) and aerobic training (AT) sessions at self-selected intensity. Participants were 54 recreationally trained subjects assigned to either RT or AT groups. During RT, participants performed three sets of 10 repetitions of each exercise at a self-selected intensity (load). After RT completion, participants performed one repetition maximum (1RM) and 10RM tests. During AT, participants performed a treadmill exercise at a self-selected intensity and duration (velocity and time). After AT completion, participants performed a treadmill maximal exercise test using a ramp protocol. During RT, subjects chose an intensity (43.6%-60.2% 1RM) below typical training recommendations, and RPE increased in successive exercise sets. During AT, participants chose an intensity (83.9% Heart Ratepeak) in line with typical training recommendations, and RPE increased from the first to second quartile of the session (from a mean of 3.9, standard deviation [ SD] = 1.7 to a mean of 5.4, SD = 1.7; p < .05) and remained stable thereafter. These recreationally trained participants self-selected lower RT intensities than are typically recommended for strength and hypertrophy increases (>67% 1RM) and AT intensities in line with typically recommended intensity (64%-95% HRmax) for cardiovascular fitness increases. Thus, for recreational trained individuals to perform RT at recommended intensities, specific instruction will be required.
Asunto(s)
Ejercicio Físico/fisiología , Esfuerzo Físico/fisiología , Entrenamiento de Fuerza/métodos , Adulto , Prueba de Esfuerzo , Femenino , Humanos , Masculino , Levantamiento de Peso/fisiología , Adulto JovenRESUMEN
Newly synthesized histones H3 and H4 undergo a cascade of maturation steps to achieve proper folding and to establish post-translational modifications prior to chromatin deposition. Acetylation of H4 on lysines 5 and 12 by the HAT1 acetyltransferase is observed late in the histone maturation cascade. A key question is to understand how to establish and regulate the distinct timing of sequential modifications and their biological significance. Here, we perform proteomic analysis of the newly synthesized histone H4 complex at the earliest time point in the cascade. In addition to known binding partners Hsp90 and Hsp70, we also identify for the first time two subunits of the histone acetyltransferase inhibitor complex (INHAT): PP32 and SET/TAF-Iß. We show that both proteins function to prevent HAT1-mediated H4 acetylation in vitro. When PP32 and SET/TAF-Iß protein levels are down-regulated in vivo, we detect hyperacetylation on lysines 5 and 12 and other H4 lysine residues. Notably, aberrantly acetylated H4 is less stable and this reduces the interaction with Hsp90. As a consequence, PP32 and SET/TAF-Iß depleted cells show an S-phase arrest. Our data demonstrate a novel function of PP32 and SET/TAF-Iß and provide new insight into the mechanisms regulating acetylation of newly synthesized histone H4.
Asunto(s)
Histona Acetiltransferasas/metabolismo , Chaperonas de Histonas/metabolismo , Histonas/metabolismo , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Factores de Transcripción/metabolismo , Acetilación , Western Blotting , Proteínas de Unión al ADN , Proteínas HSP90 de Choque Térmico/metabolismo , Células HeLa , Chaperonas de Histonas/genética , Histonas/genética , Humanos , Péptidos y Proteínas de Señalización Intracelular/genética , Lisina/genética , Lisina/metabolismo , Espectrometría de Masas , Proteínas Nucleares , Unión Proteica , Proteómica , Interferencia de ARN , Proteínas de Unión al ARN , Factores de Transcripción/genéticaRESUMEN
O objetivo foi verificar e comparar o efeito da prática de exercício físico na aptidão física em idosos, com prática de atividades físicas diferenciadas e específicas. Como metodologia, foram formados 3 grupos de idosos e realizados testes por meio de protocolos e anamnese. Os resultados nos mostram que a atividade física regular nos grupos ativos apresenta diferença significativa no desempenho global quando comparado com o grupo sedentário. Concluiu-se que a prática de atividade física ou lúdica regular melhora o desempenho global dos idosos.
The objective was to verify and compare the effect of physical exercise practice on physical fitness in the elderly, with practice of differentiated and specific physical activities. As a methodology, 3 groups of elderly were formed and performed tests through protocols and anamnesis. The results show that regular physical activity in the active groups presents a significant difference in overall performance when compared to the sedentary group. It was concluded that the practice of regular physical activity or play improves the overall performance of the elderly.
El objetivo fue verificar y comparar el efecto de la práctica de ejercicio físico en la aptitud física en ancianos, con práctica de actividades físicas diferenciadas y específicas. Como metodología, se formaron 3 grupos de ancianos y se realizaron pruebas a través de protocolos y anamnesis. Los resultados nos muestran que la actividad física regular en los grupos activos presenta una diferencia significativa en el desempeño global en comparación con el grupo sedentario. Se concluyó que la práctica de actividad física o lúdica regular mejora el desempeño global de los ancianos.
Asunto(s)
Humanos , Persona de Mediana Edad , Anciano , Anciano , Actividad Motora , Aptitud Física , Promoción de la SaludRESUMEN
With 23,398 fitness academies, catering for around seven million users, Brazil ranks second behind the United States in the number of units. The objective of this study is to delineate the profile of supervised physical activity practitioners in the three most southern states of Brazil (Paraná, Santa Catarina, and Rio Grande do Sul), analyzing the reasons which lead them to choose a fitness service. Fitness academy clients were also surveyed in relation to their alcohol, tobacco and food supplement usage. Seven hundred and ninety-seven (797) people were evaluated by means of a questionnaire. Data was collected in 24 fitness academies (eight in each state) in the three most southern states of Brazil. It was observed that those who practiced exercise regularly at fitness academies smoked little, consumed few food supplements, and with (50.44%) of those surveyed stating that they consumed only moderate amounts of alcohol. The majority, (34.13%) of exercise practitioners in southern Brazil chose to go to the gym five times a week, (40.99%) went at night, with (40.56%) giving as their reason for practicing exercise as being a way to improve their health. The distance between their home and the gym was the most important reason in choosing a place to conduct training. Intense individual activities were the most practiced type of exercise, there being no statistical difference between the percentages in the three states.
O Brasil ocupa o segundo lugar do mundo em número de academias, totalizando 23.398 unidades com cerca de sete milhões de frequentadores, ficando atrás apenas dos EUA. Neste sentido, o objetivo desse estudo foi delinear o perfil dos praticantes de atividade física supervisionada, analisando os motivos que os levam a escolher um serviço de fitness, bem como traçar o perfil de consumo de álcool, tabaco e suplementos alimentares dos habitantes da Região Sul do Brasil. Foram avaliadas 797 pessoas através de um questionário próprio. A coleta foi realizada em 24 academias divididas em oito para cada um dos três estados do Sul do Brasil. Observou-se que os frequentadores de academias da área de abrangência da pesquisa fumam pouco, consomem poucos suplementos alimentares e apenas 50,44% dessa população consome bebidas alcoólicas de maneira moderada. A maior parte (34,13%) dos praticantes de exercício físico do Sul do Brasil optam por frequentar a academia cinco vezes por semana, no período noturno (40,99%), com o intuito de melhorar a saúde (40,56%). A distância entre a residência e a academia se mostrou o motivo mais relevante para escolher um locar para realizar o treinamento. O tipo de exercício mais praticado são as atividades individuais mais intensas e não houve diferença estatística entre os percentuais nos três estados estudados.
Asunto(s)
Nicotiana , Ejercicio Físico , Suplementos Dietéticos , Centros de Acondicionamiento , EtanolRESUMEN
Power efficiency is critical for electrical stimulators. Battery life of wearable stimulators and wireless power transmission in implanted systems are common limiting factors. Boost DC/DC converters are typically needed to increase the supply voltage of the output stage. Traditionally, boost DC/DC converters are used with fast control to regulate the supply voltage of the output. However, since stimulators are acting as current sources, such voltage regulation is not needed. Banking on this, this paper presents a DC/DC conversion strategy aiming to increase power efficiency. It compares, in terms of efficiency, the traditional use of boost converters to two alternatives that could be implemented in future hardware designs.
RESUMEN
One of the most important movements performed by the humans is gait. Biomechanical Gait analysis is usually by optical capture systems. However, such systems are expensive and sensitive to light and obstacles. In order to reduce those costs a system based on Inertial Measurements Units (IMU) is proposed. IMU are a good option to make movement analisys indoor with a low post-processing data, allowing to connect those systems to an Android platform. The design is based on two elements: a) The IMU sensors and the b) Android device. The IMU sensor is simple, small (35 x 35 mm), portable and autonomous (7.8 hrs). A resolution of 0.01° in their measurements is obtained, and sends data via Bluetooth link. The Android application works for Android 4.2 or higher, and it is compatible with Bluetooth devices 2.0 or higher. Three IMU sensors send data to a Tablet wirelessly, in order to evaluate the angles evolution for each joint of the leg (hip, knee and ankle). This information is used to calculate gait index and evaluate the gait quality online during the physical therapist is working with the patient.
RESUMEN
Introdução: O volume em uma sessão de treinamento de força (TF) pode alterar a flexibilidade. Objetivo: Verificar o efeito agudo de uma sessão de TF com diferentes volumes na flexibilidade, imediatamente após o término da sessão e 24 e 48 horas após. Métodos: Sessenta e um voluntários (24,31±0,81anos) do sexo masculino distribuídos aleatoriamente em três grupos: grupo controle (GC), duas série (G2S) e três séries (G3S). Todos os grupos foram avaliados pré e pós-treinamento no teste de sentar e alcançar, Goniometria (ombro, cotovelo, quadril, joelho e coluna) e teste de 10 Repetições Máximas (RM). O treinamento foi composto por nove exercícios que envolviam todas as articulações avaliadas e executado 10 repetições. Resultados: Houve aumento dos níveis de flexibilidade para maioria dos movimentos do ombro; quadril (extensão) e tronco (flexão e extensão) quando comparadas as situações pré e pós-treinamento para todos os grupos submetidos ao treinamento (p < 0,05). Conclusão: Uma sessão de ER com diferentes volumes é capaz de modificar a flexibilidade de articulações triaxiais.
Introduction: The volume in a resistance exercise (RE) session can change flexibility. Objective: The aim of the study was to investigate the acute effect of a training session with different volumes flexibility, immediately after the session and 24 and 48 hours after the training session. Methods: Study participants were 61 volunteers (24.31 ± 0,81 year) males randomly assigned to three groups: control group (CG), two series (G2S) and three series (G3S). All groups were assessed before and after training in the sit and reach test, Goniometry (shoulder, elbow, hip, knee and spine) and test 10 Maximum Repetitions (RM). The training was composed of nine exercises that involved all tested joints and was run 10 repetitions of each exercise with moderate intensity. Results: There was an increase of the flexibility levels for most shoulder movements; hip (extension) and trunk (flexion and extension) when comparing before and after training for all undergoing training groups (p <0.05). Conclusion: A RE session with different volumes is able to modify the flexibility of triaxial joints.
Asunto(s)
Humanos , Masculino , Adulto , Adulto Joven , Rango del Movimiento Articular , Entrenamiento de Fuerza , Articulación del Hombro , Ejercicio Físico , Torso , Articulación de la CaderaRESUMEN
Histone post-translational modifications are key contributors to chromatin structure and function, and participate in the maintenance of genome stability. Understanding the establishment and maintenance of these marks, along with their misregulation in pathologies is thus a major focus in the field. While we have learned a great deal about the enzymes regulating histone modifications on nucleosomal histones, much less is known about the mechanisms establishing modifications on soluble newly synthesized histones. This includes methylation of lysine 9 on histone H3 (H3K9), a mark that primes the formation of heterochromatin, a critical chromatin landmark for genome stability. Here, we report that H3K9 mono- and dimethylation is imposed during translation by the methyltransferase SetDB1. We discuss the importance of these results in the context of heterochromatin establishment and maintenance and new therapeutic opportunities in pathologies where heterochromatin is perturbed.
Asunto(s)
Histonas/metabolismo , Lisina/metabolismo , Biosíntesis de Proteínas , Procesamiento Proteico-Postraduccional , Células HeLa , N-Metiltransferasa de Histona-Lisina/metabolismo , Histonas/química , Humanos , Metilación , Proteína Metiltransferasas/metabolismo , Ribosomas/enzimologíaRESUMEN
Concurrent training (CT) has been widely used in fitness centers to simultaneously optimize cardiovascular and neuromuscular fitness, and induce a high-energy expenditure. Therefore, the aim of this study was to compare the acute effects of 2 different orders of CT on hormonal responses in concurrently trained men. Fourteen men (mean ± SD: 24.7 ± 5.1 years) were randomly divided into 2 groups: endurance training followed by strength (ES, n = 7) and strength training followed by endurance (SE, n = 7). Serum concentrations of testosterone, cortisol, growth hormone, and IGF-1 binding protein 3 (IGFBP-3) were measured before and after both training orders. A significant interaction between exercise order and time was only found in the IGFBP-3 levels (p = 0.022). The testosterone and IGFBP-3 concentrations significantly increased in the ES group after the exercise trainings (57.7 ± 35.1%, p = 0.013 and 17.0 ± 15.5%, p = 0.032, respectively) but did not change significantly in the SE group (15.5 ± 36.6%, p = 0.527 and -4.2 ± 13.9%, p = 0.421, respectively). Conversely, cortisol and growth hormone concentrations significantly increased in both ES (169.2 ± 191.0%, p = 0.021 and 13,296.8 ± 13,009.5%, p = 0.013, respectively) and SE (92.2 ± 81.5%, p = 0.017 and 12,346.2 ± 9714.1%, p = 0.001, respectively) groups compared with baseline values. No significant correlations were found between the changes in the hormonal concentrations. In conclusion, these results suggest that immediately postexercise testosterone and IGFPB-3 responses are significantly increased only after the ES order. Therefore, an ES training order should be prescribed if the main focus of the training intervention is to induce an acute postexercise anabolic environment.
Asunto(s)
Hormona de Crecimiento Humana/sangre , Hidrocortisona/sangre , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Resistencia Física/fisiología , Entrenamiento de Fuerza/métodos , Testosterona/sangre , Adulto , Biomarcadores/metabolismo , Metabolismo Energético , Humanos , MasculinoRESUMEN
The purposes of this study were to characterize the achievement goals and sportsmanlike attitudes in young soccer players and their association with perceived pressure from different significant social agents (parents/family, coaches, teammates and friends). The sample of the study was comprised of 118 young soccer players, aged between 11 and 19 years (M= 14.68, SD= 2.16). Athletes had 5.40 ± 2.39 years of sport experience and 71 (60.2%) of the athletes had competed at a regional level, while 47 (39.8%) had participated in national competitions. Participants completed a socio-demographic survey, the Task and Ego Orientation in Sports Questionnaire and the Sports Values Questionnaire. The statistical analyses involved univariate normality and descriptive, comparative and correlational analyses. Results revealed that task orientation was positively correlated with sportsmanlike attitudes (r= 0.47, p<0.01) and negatively associated with unsportsmanlike attitudes (r= −0.46, p<0.01), whereas ego orientation effects were contrary (sportsmanlike attitudes: r= −0.33, p<0.01 and unsportsmanlike attitudes: r= 0.42, p<0.01). Perceived pressures from all significant social agents were positively and significantly associated with unsportsmanlike attitudes (p<0.05), with perceived pressure from coaches also being associated with higher ego orientation scores and with lower task orientation and sportsmanlike attitudes in young soccer players. In summary, these results indicate the need for coaches and other social agents to promote a competition climate that reinforces self-referenced improvements and the expression of positive social attitudes in sports contexts. .
- Os objetivos do estudo foram caracterizar as orientações motivacionais e as atitudes (anti)desportivas de jovens futebolistas e suas associações com as pressões percebidas de distintos agentes sociais (pais/família, treinadores, colegas de equipa e amigos). A amostra foi constituída por um total de 118 futebolistas masculinos, com idades entre os 11 e os 19 anos (M= 14.68, DP= 2.16). Os atletas tinham 5.40 ± 2.39 anos de experiência federada, sendo que 71 (60.2%) atletas competiam em nível regional e 47 (39.8%) em competições nacionais. Os participantes responderam a um questionário sociodemográfico, ao Questionário de Orientação Motivacional no Desporto e ao Questionário de Valores no Desporto. A análise estatística envolveu procedimentos de normalidade univariada e análise descritiva, comparativa e correlacional. Os resultados indicaram que a orientação para a tarefa correlacionou-se positivamente com as atitudes desportivas (r= 0.47, p<0.01) e negativamente com as atitudes antidesportivas (r= −0.46, p<0.01), enquanto a orientação para o ego exerceu um efeito contrário (atitudes desportivas: r= −0.33, p<0.01 e atitudes antidesportivas: r= 0.42, p<0.01). As pressões percebidas por parte de todos os agentes de socialização associaram-se positiva e significativamente com as atitudes antidesportivas (p<0.05), sendo os treinadores as principais fontes de pressão promotoras de níveis superiores de orientação para o ego e menores níveis de orientação para a tarefa e atitudes desportivas nos atletas. Em suma, estes resultados indicam a necessidade dos treinadores e outros agentes sociais promoverem um clima de competição orientado para o autoaperfeiçoamento e para a expressão de atitudes socialmente positivas ...
RESUMEN
The objective of this study was to analyze the influence of positional release therapy (PRT) on the myofascial tension of the upper trapezius muscle with an active myofascial trigger point (TrP). We studied 30 subjects (18 men and 12 women), mean age 34.5 ± 9.4 years, with an active TrP in the upper trapezius muscle on one side. A search for TrPs was performed bilaterally and the points were considered to be active when both local and referred pain evoked by manual palpation reproduced a deep aching and burning pain. The patients were evaluated under three conditions: (a) resting baseline, (b) concentric contraction and (c) isometric contraction, before and after treatment with PRT, regarding the following parameters: (i) pain intensity during manual palpation (visual analogue pain scale) and (ii) upper trapezius muscle electromyographic (EMG) signals. A significant decrease in painful symptoms from 5.3 ± 1.9 to 2.8 ± 1.8 (p < 0.001) was observed after treatment. There were no significant differences in EMG signals during resting baseline and in the presence of concentric contraction after the PRT session. It was concluded that PRT may be an effective treatment for pain relief and to reduce resting baseline EMG signals in the upper trapezius muscle with a TrP, suggesting that its use as an alternative or an adjunct to other therapies. The effectiveness of this type of treatment should be confirmed by further clinical studies.
O objetivo deste estudo foi analisar a influência da Terapia de Libertação Posicional (TLP) sobre a tensão miofascial do músculo trapézio superior, com presença de ponto gatilho (PG) miofascial ativo. Foram estudados 30 indivíduos (18 homens e 12 mulheres), idade média 34,5 + 9,4 anos, com presença de PG ativo, no músculo trapézio superior, de um dos lados. Os PG foram avaliados em ambos os lados e foram considerados ativos quando era evocada uma dor local, disseminada e persistente, por palpação manual. Os indivíduos foram avaliados em três condições: (a) repouso basal, (b) contração concêntrica e (c) contração isométrica, antes e após da aplicação da TLP, nos seguintes parâmetros: (i) intensidade da dor durante a palpação (escala visual analógica de dor) e (ii) sinais eletromiográficos (EMG) do músculo trapézio superior. Houve uma redução significativa do sintoma doloroso 5,3 ± 1,9 para 2,8 ± 1,8 (p < 0,001). Quanto à atividade electromiográfica, em repouso basal e na contração concêntrica, não se observaram diferenças significativas nos sinais EMGs, após a utilização da TLP. Os resultados sugerem que a TLP diminui o sintoma doloroso e reduz os sinais da electromiografia, em repouso basal, do músculo trapézio superior com PG. Isto sugere que a técnica de TLP pode ser utilizada como uma alternativa ou em concomitância com outras terapias. A eficácia desta forma de tratamento deve ser confirmada por outros estudos clínicos.