RESUMEN
In this paper, a bibliometric analysis of research on wearable technologies for cardiovascular diseases was conducted with Bibliometrix and based on 1675 papers collected in Scopus from 2000 to 2022, and interesting results were presented. The US and China stood out for their sustained productivity in the field of cardiovascular diseases. The examination of the authors' keywords revealed that between 2000-01 and 2009-12, "Respiration" and "personalized applications" were the most popular research topics, however from 2010-01 to 2019-12, attention was given to "ECG," "Wearable devices," "Wearable sensors," and "heart rate". This study suggests that in the present decade, researchers should focus on topics related to artificial intelligence, hypertension, ECG, wearable sensors, and African countries must address their technological gap in the coming years.
Asunto(s)
Enfermedades Cardiovasculares , Dispositivos Electrónicos Vestibles , Humanos , Inteligencia Artificial , Enfermedades Cardiovasculares/diagnóstico , Enfermedades Cardiovasculares/terapia , Bibliometría , TecnologíaRESUMEN
The metallothionein (MT)3 gene is expressed predominantly in the brain and the organs of the reproductive system, and fails to respond to metal ions in vivo. A CTG repeat was proposed to function as a potential repressor element in nonpermissive cells, and a sequence similar to the JC virus silencer element was found to function as a negative element in permissive primary astrocytes. The objective of this study was to characterize further the mechanisms governing cell-type specific MT-3 gene transcription. We searched for a suitable cell line expressing the MT-3 gene to be used for determination of MT-3 promoter tissue specificity, and showed that MT-3 expression is activated during neuroectodermal differentiation of P19 cells induced by retinoic acid to levels similar to those found in whole brain. Deletion of the CTG repeat or of the JC virus silencer did not promote MT-3 promoter activity in nonpermissive cells, or enhance expression in permissive cells. We identified MT-3 promoter sequences interacting with liver and brain nuclear proteins, as assayed by DNase I footprinting analyses and electrophoretic mobility shift assay, and assessed the role of these sequences in the regulation of MT-3 expression by cotransfection experiments. We generated stable transfectants in permissive C6 and nonpermissive NIH-3T3 cells, and analysed the methylation status of the MT-3 gene. These studies show that regulation of tissue-specific MT-3 gene expression does not appear to involve a repressor, and suggest that other mechanisms such as chromatin organization and epigenetic modifications could account for the absence of MT-3 gene transcription in nonpermissive cells.