RESUMEN

Despite the availability of a Mycobacterium bovis bacille Calmette Guérin (BCG) vaccine, tuberculosis (TB) remains a global public health problem. In this study, we introduced the c-di-GMP phosphodiesterase gene Rv1357c, implicated in regulating mycobacterial replication within macrophages, into BCG Pasteur, and tested the resulting strain for its capacity to serve as a vaccine against TB in a murine model. Modified BCG was more phagocytosed than its parental strain, but halted bacterial replication, and protected against M. tuberculosis challenge similarly to unmodified BCG.

Asunto(s)

Aciltransferasas/inmunología , Vacuna BCG/inmunología , Mycobacterium tuberculosis/inmunología , Tuberculosis Pulmonar/prevención & control , Vacunación , Aciltransferasas/genética , Animales , Línea Celular , Modelos Animales de Enfermedad , Macrófagos/inmunología , Macrófagos/microbiología , Ratones , Ratones Endogámicos BALB C , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/crecimiento & desarrollo , Fagocitosis , Factores de Tiempo , Tuberculosis Pulmonar/inmunología , Tuberculosis Pulmonar/microbiología , Vacunas Sintéticas/inmunología