RESUMEN
Polyphenols are plant secondary metabolites that function mostly as a general stress-induced protective mechanism. Polyphenols have also gained interest due to their beneficial properties for human health. Strawberry leaves represent an agro-industrial waste material with relevant bioactive polyphenol content, which could be incorporated into circular economy strategies. However, due to the low quantities of polyphenols in plants, their production needs to be improved for cost-effective applications. The objective of this research was to compare polyphenol production in strawberry (Fragaria × ananassa cv. Festival) leaves in plants grown in greenhouse conditions and plants grown in vitro, using three possible elicitor treatments (UV irradiation, cold exposure, and cysteine). General vegetative effects were morphologically evaluated, and specific polyphenolic compounds were quantified by UHPLC-DAD-MS/MS. Gallic acid was the most abundant polyphenol found in the leaves, both in vivo and in vitro. The results showed higher amounts and faster accumulation of polyphenols in the in vitro regenerated plants, highlighting the relevance of in vitro tissue culture strategies for producing compounds such as polyphenols in this species and cultivar.
Asunto(s)
Fragaria , Hojas de la Planta , Polifenoles , Fragaria/química , Fragaria/metabolismo , Polifenoles/química , Hojas de la Planta/química , Espectrometría de Masas en Tándem , Cromatografía Líquida de Alta Presión , Ácido Gálico/químicaRESUMEN
Resumen Las orquídeas del género Vanda tienen gran valor en el mercado ornamental por la vistosidad de sus flores. El mejoramiento genético en vandas es mayormente realizado a través de métodos convencionales, pero la alta demanda del mercado actual sobre nuevos cultivares hace necesaria la inclusión de técnicas moleculares y biotecnológicas en la mejora genética. El objetivo de esta revisión, es analizar la utilidad de diferentes herramientas moleculares y biotecnológicas para el desarrollo de un programa de mejoramiento genético en Vanda analizar la utilidad de diferentes herramientas moleculares y biotecnológicas para el desarrollo de un programa de mejora genética en Vanda, mediante la interrelación con biología reproductiva y mejoramiento convencional en el género. En esta revisión se consultaron un total de 95 artículos publicados en revistas científicas. Como resultado se brinda una compilación de información de utilidad como material de consulta para técnicos, profesionales e investigadores relacionados con esta temática.
Abstract Orchids of the Vanda genus have great value in the ornamental market due to the showiness of their flowers. Genetic improvement in this genus, is mostly carried out through conventional methods, but the high demand of the current market for new cultivars makes it necessary to include molecular and biotechnological techniques in genetic improvement. The aim of this review is to analyze the usefulness of different molecular and biotechnological tools for the development of a breeding program in Vanda, through the interrelation with reproductive biology and conventional breeding in the genus. A total of 95 articles published in scientific journals were consulted in this review. As a result, a compilation of information is provided that can serve as reference material for technicians, professionals and researchers related to this subject.