RESUMEN

This article presents a case of successful treatment of inoperable gastric cancer with impaired mismatched nucleotide repair system (dMMR/MSI-H) in a 72-year-old patient. In view of age, somatic status and the presence of comorbidity, it was decided to prescribe anti-PD-1 therapy in the 1st line of treatment. Currently, after a 2-year course of treatment, the patient is in stable remission.

Asunto(s)

Neoplasias Colorrectales , Neoplasias Gástricas , Humanos , Anciano , Neoplasias Gástricas/genética , Reparación de la Incompatibilidad de ADN , Inestabilidad de Microsatélites , ADN

RESUMEN

Previously we've described the obtainment of a subpopulation of cancer stem cells from a human colorec- tal carcinoma cell line MIP101. These cells possess elevated clonogenic and tumorigenic capacities. According to our data, depletion of stem compartment in a cancer cell population blocks its tumorigenicity. The current work is dedicated to the comparison of tumorigenic potential between cell populations with enriched or depleted stem compartment. We show that tumor growth following xenografting of enriched stem cell population can be suppressed by intramuscular injections of ganciclovir. Thus, we report a method to obtain a cell population with high Oct4 promoter expression within the MIP101 colorectal carcinoma cell line and to eliminate these cells from the population in vitro as well as in vivo.

Asunto(s)

Biomarcadores de Tumor/biosíntesis , Neoplasias Colorrectales/patología , Neoplasias Colorrectales/prevención & control , Células Madre Neoplásicas/citología , Células Madre Neoplásicas/efectos de los fármacos , Factor 3 de Transcripción de Unión a Octámeros/biosíntesis , Animales , Biomarcadores de Tumor/genética , Técnicas de Cultivo de Célula , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Neoplasias Colorrectales/metabolismo , Ganciclovir/farmacología , Ganciclovir/uso terapéutico , Vectores Genéticos , Humanos , Lentivirus/genética , Ratones , Ratones Desnudos , Células Madre Neoplásicas/metabolismo , Células Madre Neoplásicas/patología , Factor 3 de Transcripción de Unión a Octámeros/genética , Puromicina/farmacología , Ensayos Antitumor por Modelo de Xenoinjerto