RESUMEN
In recent years high-field Dynamic Nuclear Polarization (DNP) enhanced NMR spectroscopy has gained significant interest. In high-field DNP-NMR experiments (⩾400MHz 1H NMR, ⩾9.4T) often a stand-alone gyrotron is used to generate high microwave/THz power to produce sufficiently high microwave induced B1e fields at the position of the NMR sample. These devices typically require a second, stand-alone superconducting magnet to operate. Here we present the design and realization of a ferroshim insert, to create two iso-centers inside a commercially available wide-bore NMR magnet. This work is part of a larger project to integrate a gyrotron into NMR magnets, effectively eliminating the need for a second, stand-alone superconducting magnet.
Asunto(s)
Espectroscopía de Resonancia Magnética/instrumentación , Superconductividad , Algoritmos , Diseño de Equipo , MicroondasRESUMEN
High-resolution proton NMR spectroscopy is well-established as a tool for metabolomic analysis of biological fluids at the macro scale. Its full potential has, however, not been realised yet in the context of microfluidic devices. While microfabricated NMR detectors offer substantial gains in sensitivity, limited spectral resolution resulting from mismatches in the magnetic susceptibility of the sample fluid and the chip material remains a major hurdle. In this contribution, we show that susceptibility broadening can be avoided even in the presence of substantial mismatch by including suitably shaped compensation structures into the chip design. An efficient algorithm for the calculation of field maps from arbitrary chip layouts based on Gaussian quadrature is used to optimise the shape of the compensation structure to ensure a flat field distribution inside the sample area. Previously, the complexity of microfluidic NMR systems has been restricted to simple capillaries to avoid susceptibility broadening. The structural shimming approach introduced here can be adapted to virtually any shape of sample chamber and surrounding fluidic network, thereby greatly expanding the design space and enabling true lab-on-a-chip systems suitable for high-resolution NMR detection.
Asunto(s)
Dispositivos Laboratorio en un Chip , Técnicas Analíticas Microfluídicas/instrumentación , Diseño de Equipo , Europio/química , Glucosa/química , Espectroscopía de Resonancia Magnética , Modelos Teóricos , Método de Montecarlo , Agua/químicaRESUMEN
Inductively coupled planar resonators offer convenient integration of high-resolution NMR spectroscopy with microfluidic lab-on-a-chip devices. Planar spiral resonators are fabricated lithographically either by gold electroplating or by etching Cu laminated with polyimide. Their performance is characterized by NMR imaging as well as spectroscopy. A single-scan limit of detection LOD(t) = 0.95 nmol s(1/2) was obtained from sample volumes around 1 µL. The sensitivity of this approach is similar to that obtained by microstripline and microslot probes.