RESUMEN
A murine sarcoma virus (MSV) was recovered from an (NFS X NS.C58v-1) F1 mouse which developed splenic sarcoma and erythroleukemia 6 months after inoculation with a mink cell focus-inducing murine leukemia virus (MuLV) isolated from an NFS mouse infected with a wild mouse ecotropic MuLV. The MSV, designated NS.C58 MSV-1, induced foci of transformation in mouse and rat fibroblasts, and inoculation of mice of various strains 2 weeks of age or younger resulted in erythroleukemia and sarcomatous lesions in spleen, lymph node, and brain. The MSV provirus was molecularly cloned from a genomic library prepared from transformed non-producer rat cells. The 8.8-kilobase proviral DNA contained a 1.0-kilobase p21 ras coding segment which replaced most of the gp70-encoding portion of an MuLV, most likely the endogenous C58v-1 ecotropic virus. The ras oncogene is closely related to v-Ha-ras by hybridization, expression of p21 protein, and nucleotide sequence. It is nearly identical in sequence to v-bas, the only previously described transduced, activated mouse c-ras. At position 12 in the p21 coding region, arginine is substituted for the naturally occurring glycine present in c-ras. A second MSV isolate is described which is similar to NS.C58 MSV-1 except for a 100- to 200-base-pair deletion in the noncoding region of the ras-containing insert.
Asunto(s)
Genes Virales , Virus Helper/aislamiento & purificación , Hemangiosarcoma/microbiología , Virus de la Leucemia Murina/aislamiento & purificación , Oncogenes , Virus del Sarcoma Murino/aislamiento & purificación , Neoplasias del Bazo/microbiología , Animales , Neoplasias Encefálicas/microbiología , Transformación Celular Viral , Virus Helper/genética , Virus de la Leucemia Murina/genética , Leucemia Eritroblástica Aguda/microbiología , Linfoma no Hodgkin/microbiología , Ratones , Ratones Endogámicos/microbiología , Virus Inductores de Focos en Células del Visón/aislamiento & purificación , Proteína Oncogénica p21(ras) , Proteínas Oncogénicas Virales/genética , Virus del Sarcoma Murino/genética , Homología de Secuencia de Ácido Nucleico , Transducción GenéticaRESUMEN
The nucleotide sequences of the envelope genes of an African and a North American acquired immunodeficiency syndrome (AIDS) viral isolate have been determined. When their deduced amino acid sequences were aligned with the envelopes of the lymphoadenopathy and AIDS-associated retrovirus isolates, conserved and divergent regions were readily identified. Hypervariable stretches of 28-74 amino acids, exhibiting 20-30% amino acid identity at each position and characterized by reciprocal insertions and deletions, were confined to the gp120 external envelope protein. The origin and clinical implications of the AIDS viral env gene diversity are discussed.