RESUMEN
The immediate goal of this study was to develop and validate a noninvasive, computational surface mapping approach for measuring scapular kinematics by using available motion capture technology in an innovative manner. The long-term goal is to facilitate clinical determination of the role of the scapula in children with brachial plexus birth palsy (BPBP). The population for this study consisted of fourteen healthy adults with prominent scapulae. Subject-specific scapular templates were created using the coordinates of five scapular landmarks obtained from palpation with subjects seated and arms relaxed in a neutral position. The scapular landmarks were re-palpated and their locations recorded in the six arm positions of the modified Mallet classification. The six Mallet positions were repeated with approximately 300 markers covering the scapula. The markers formed a surface map covering the tissue over the scapula. The scapular template created in the neutral position was iteratively fit to the surface map of each trial, providing an estimate of the orientation of the scapula. These estimates of scapular orientation were compared to the known scapular orientation determined from the scapular landmarks palpated in each Mallet position. The magnitude of the largest mean difference about an anatomical axis between the two measures of scapular orientation was 3.8° with an RMS error of 5.9°. This technique is practical for populations with visibly prominent scapulae (e.g., BPBP patients), for which it is a viable alternative to existing clinical methods with comparable accuracy.
Asunto(s)
Neuropatías del Plexo Braquial , Modelos Biológicos , Movimiento , Rango del Movimiento Articular , Escápula/fisiopatología , Adulto , Fenómenos Biomecánicos , Neuropatías del Plexo Braquial/diagnóstico , Neuropatías del Plexo Braquial/patología , Neuropatías del Plexo Braquial/fisiopatología , Niño , Preescolar , Femenino , Humanos , Lactante , Recién Nacido , MasculinoRESUMEN
Recognition of conspecifics is an essential precursor of sexual reproduction. Most mammals and birds learn salient features of their parents or siblings early in ontogeny and later recognize individuals whose phenotypes match the mental image (template) of relatives closely enough as conspecifics. However, the young of brood parasites are reared among heterospecifics, so social learning will yield inappropriate species recognition templates. Initially, it was inferred that conspecific recognition in brood parasites depended on genetically determined templates. More recently it was demonstrated that learning plays a critical role in the development of parasites' social preferences. Here we propose a mechanism that accommodates the interaction of learned and genetic components of recognition. We suggest that conspecific recognition is initiated when a young parasite encounters some unique species-specific signal or "password" (e.g. a vocalization, behaviour or other characteristic) that triggers learning of additional aspects of the password-giver's phenotype. We examined the possibility that nestlings of the obligately brood-parasitic brown-headed cowbird (Molothrus ater) could use a species-specific vocalization, the "chatter", as a password. We found that six-day-old nestlings responded (begged) significantly more frequently to playbacks of chatters than to other avian sounds and that two-month-old fledglings approached playbacks of chatters more quickly than vocalizations of heterospecifics. Free-living cowbird fledglings and adults also approached playbacks of chatters more often than control sounds. Passwords may be involved in the ontogeny of species recognition in brood parasites generally.
Asunto(s)
Pájaros Cantores , Vocalización Animal , Animales , Especificidad de la EspecieRESUMEN
A single-case analysis was used to assess the effects of imaginal exposure in a 57-year-old woman suffering from current and reactivated post-traumatic stress disorder (PTSD) following a transient ischemic attack. The client's responses to self-reported depression, anxiety, and PTSD symptoms were repeatedly recorded during four phases: (a) initial psychotherapy, (b) imaginal exposure, (c) skill generalization, and (d) fading of treatment. In addition to dramatic reduction in levels of depression and anxiety, results showed a significant improvement in PTSD symptoms relating to recent and remote traumatic experiences. Improvements were maintained approximately 16 months after imaginal exposure ended, despite ongoing external stressors.
Asunto(s)
Imágenes en Psicoterapia , Trastornos por Estrés Postraumático/terapia , Adolescente , Abuso Sexual Infantil/psicología , Femenino , Humanos , Ataque Isquémico Transitorio/psicología , Persona de Mediana Edad , Recurrencia , Trastornos por Estrés Postraumático/psicologíaRESUMEN
The relationship between coercion strategies used by perpetrators of childhood sexual abuse (CSA) and elevations of CSA survivors on the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) was investigated. Participants were 151 women survivors of CSA in outpatient treatment at a university-based community mental health center. Scores on the MMPI-2 clinical scales and the Keane posttraumatic stress disorder (PTSD) scale were examined. Main effects were found for promised or received rewards on several clinical scales and the PTSD scale of the MMPI-2, independent of the presence of force. Specifically, the presence of such rewards was associated with significantly higher levels of symptomatology on Paranoia (Pa), Psychasthenia (Pt), Schizophrenia (Sc), and PTSD (Pk). There were no main or interaction effects noted for the presence of actual or threatened force on any of the scales.
Asunto(s)
Abuso Sexual Infantil/psicología , Coerción , MMPI/estadística & datos numéricos , Sobrevida/psicología , Adolescente , Adulto , Niño , Femenino , Humanos , Persona de Mediana Edad , Motivación , Psicometría , Reproducibilidad de los ResultadosRESUMEN
The Kaufman Adolescent and Adult Intelligence Test (KAIT), introduced in 1993, is a relatively new measure for the assessment of intelligence. Because of the test's theoretical foundation, standardization procedure, test items, and score interpretations, it is assumed to be a culturally sensitive measure. However, there is little supporting clinical or empirical evidence. The present case study examined cross-cultural applications of the KAIT using 3 volunteers from diverse cultural backgrounds with differential levels of American acculturation. In addition to test scores, the participants' impressions of the testing process were measured with the Subjective Units of Distress Scale. Preliminary evidence suggests that the KAIT, like its predecessor, the K-ABC, may be useful when working with culturally diverse people.
Asunto(s)
Aculturación , Comparación Transcultural , Pruebas de Inteligencia , Identificación Social , Adulto , Asiático/psicología , Emigración e Inmigración , Etnicidad/psicología , Femenino , Hispánicos o Latinos/psicología , Humanos , Japón/etnología , México/etnología , Grupos Minoritarios/psicología , Siria/etnología , Estados UnidosRESUMEN
A fluorescent method developed for visualizing gamma-glutamyltranspeptidase (GGT) in intact liver cells was adapted to leukocytes and used in a multiparameter flow cytometric study of blood and bone marrow cells from rats with subcutaneous implants of mammary carcinoma 5A. The severe granulocytosis caused by this non-metastatic tumor was preceded by a progressive rise in the percentage of leukocytes with high GGT fluorescence. Both granulocytes and small, immature cells of bone marrow showed increased GGT expression, whereas in blood this increase was attributable entirely to mature granulocytes. At 28 days (but not yet at 14 days) after carcinoma implantation, 20-30% of blood or bone marrow granulocytes constituted a distinct subpopulation in that their GGT fluorescence intensity range was much higher and did not overlap with the range for the rest of the population. The results indicate that fluorescent GGT assay of intact leukocytes provides a useful probe for flow cytometric analysis of population heterogeneity in leukoproliferative disorders.
Asunto(s)
Separación Celular , Citometría de Flujo , Leucocitos/enzimología , Neoplasias Mamarias Experimentales/enzimología , gamma-Glutamiltransferasa/metabolismo , Animales , Médula Ósea/patología , Granulocitos/enzimología , Masculino , Microscopía Fluorescente , Trasplante de Neoplasias , RatasRESUMEN
Lymphocytes from acute lymphocytic leukemia (ALL) subjects were converted by mitogens to blast-like cells whose microscopic appearance and rate of formation was indistinguishable from those in mitogen incubated control lymphocytes. In ALL lymphocytes, however, pokeweed mitogen (PWM) failed to stimulate GGT expression; the mean increase it caused in thymidine kinase (TK) activity and thymidine incorporation was normal, though there were appreciable individual variations. These variations were also apparent with concanavalin A (Con A) but, in most ALL cases, TK and thymidine incorporation rose to much higher levels than in Con-A-treated control lymphocytes. The results indicate that evaluation of the response to mitogens by quantitative biochemical criteria provides a sensitive method for revealing functional impairments in microscopically normal ALL lymphocytes.
Asunto(s)
Concanavalina A/farmacología , Activación de Linfocitos , Linfocitos/enzimología , Mitógenos de Phytolacca americana/farmacología , Leucemia-Linfoma Linfoblástico de Células Precursoras/enzimología , Timidina Quinasa/metabolismo , gamma-Glutamiltransferasa/metabolismo , Células Cultivadas , Replicación del ADN , Humanos , Cinética , Linfocitos/efectos de los fármacos , Linfocitos/inmunología , Leucemia-Linfoma Linfoblástico de Células Precursoras/inmunología , Valores de ReferenciaRESUMEN
gamma-Glutamyltranspeptidase (GGT) activity (per mg protein) in blood lymphoid cells of 27 children with acute lymphoblastic leukemia (ALL) (1.05 +/- 0.15) was significantly below that of controls (2.25 +/- 0.30), became normalized during chemotherapy-induced remission (2.47 +/- 0.26), and was low again (1.59 +/- 0.62) in relapsed subjects. Individual variations in the GGT activity of the blood lymphoid cell fraction (per mg protein) bore a significant inverse correlation to the number of white blood cells (WBC) as well as of blasts per ml blood. Blasts had minimal GGT activity; however, partial GGT deficiency was also exhibited by the microscopically normal circulatory lymphocytes of several patients prior to treatment and in relapsed subjects whose blood was still devoid of blasts. Significantly diminished GGT activity (per mg protein) was found in the blood granulocytes of ALL subjects. This deficit, restored during remission and present again at relapse, varied in magnitude but showed no statistically significant correlation to the different patients' degree of neutropenia. In about one-third of the newly diagnosed or relapsed pre-B ALL children, the circulatory granulocytes' GGT activity was only 10-20% of normal. The results suggest that 1) the presence or absence of this sign of functional maldifferentiation in granulocytes is a factor in the heterogeneity of disease manifestation among subjects with apparently the same type of ALL and that 2) measurement of GGT in the circulatory granulocytes, as well lymphocytes, may be useful for monitoring the efficacy of chemotherapy.
Asunto(s)
Granulocitos/enzimología , Leucemia Linfoide/enzimología , Linfocitos/enzimología , gamma-Glutamiltransferasa/deficiencia , Médula Ósea/enzimología , Humanos , Leucemia Linfoide/sangre , Leucemia Linfoide/tratamiento farmacológico , Recuento de Leucocitos , Pronóstico , Timidina Quinasa/metabolismoRESUMEN
The activity of thymidine kinase (TK) and the proportion of its isozymes (TK1/TK2) were studied in peripheral lymphoid cells of 37 children with acute lymphocytic leukemia (ALL). The high TK in 25 untreated subjects (31.5 +/- 8.9) decreased during chemotherapy-induced remission to uniformly low (5.3 +/- 0.4) normal values, and rose again during relapse to a mean of (24.8 +/- 8.1). The proportion of isozyme 1 followed the same pattern but TK was a more sensitive indicator of disease state. The lymphocyte fractions' TK (per mg protein) correlated with the number (per ml blood) of WBCs, blasts and lymphocytes. Although the higher TK of blasts than of apparently normal lymphocytes was confirmed in cases permitting clean physical separation, the lymphocyte fraction of several untreated subjects with minimal blast counts also exhibited elevated TK. Moreover, this elevation was also seen in relapsed cases even if their blood (unlike bone marrow) was devoid of blasts. The results indicate that quantification of TK can reveal a subpopulation of maldifferentiated lymphocytes which are microscopically normal and that it may provide an objective parameter of prognostic differences between ALL subjects with similar hematological characteristics.