RESUMEN

Cholesteryl ester transfer protein (CETP) has been shown to transfer cholesteryl esters among plasma lipoproteins. However, when reconstituted into phosphatidylcholine liposomes, the 74,000 protein mediates above non-specific values the transfer of HDL bound [3H]cholesterol into the artificial membrane system. Employing the known cDNA sequence of CETP, we synthesized a series of oligonucleotides with specific sequences for different regions of CETP and RNA isolated from tissues known to be producers of CETP (liver), and tissues not design to synthesize and secrete CETP (ovary, lung, intestine and heart). Hybridization experiments showed that independently of the type of tissue tested CETP sequences were found. It is suggested that a membrane form of CETP might have important repercussions locally.

Asunto(s)

Proteínas Portadoras/metabolismo , Colesterol/metabolismo , Glicoproteínas , Lipoproteínas HDL/metabolismo , Membranas Artificiales , Animales , Secuencia de Bases , Transporte Biológico , Proteínas Portadoras/genética , Proteínas de Transferencia de Ésteres de Colesterol , Humanos , Datos de Secuencia Molecular , Oligodesoxirribonucleótidos , ARN Mensajero/metabolismo , Conejos , Distribución Tisular