RESUMEN
AIM: Study the effect of ribosomal proteins of respiratory bacteria composing the basis of the immune-modulating preparation ribomunil on adhesive properties of buccal epithelium of healthy donors, and carry out comparison of this parameter during use of other bacterial products. MATERIALS AND METHODS: Various amounts of bacterial ribosomal proteins, Escherichia coli (serotype O127:B8) and one-day Staphylococcus aureus (strain 5983) culture supernatant were added to "buccal epitheliocytes--candida" system and incubated. Buccal cells were washed after the incubation from non-bound candida and differentiated microscopically by the amount of cells with various levels of candida adhesion. Separate effect of ribosomal proteins on buccal cells and candida was studied, as well as their impact on the production of secretory products of buccal cells. RESULTS: Buccal epitheliocytes in control adhered on average 14.6 candidiasis cells. After incubation with bacterial ribosomal proteins the index decreased by 2.3 +/- 0.2 times. During separate addition of ribomunil to buccal cells and candida, ribosomal bacterial proteins were shown to have effect only on epitheliocytes. Activity of ribosomal proteins had a selective character, as shown by the lack of effect under the influence of S. aureus supernatant on buccal cells as well as an increase of adhesion under the influence of lipopolysaccharide on epitheliocytes. Viability of cells in all the cases remained at a level of 90 - 98%. Buccal cells during contact with ribomunil produced a complex of soluble mediators that took part in its blocking effect. CONCLUSION: The increase of stability of mucosal tract to microbial adhesion is an element of innate immunity and may be one of the components of immune-protecting effect of bacterial ribosomal proteins.
Asunto(s)
Proteínas Bacterianas/farmacología , Candida albicans/fisiología , Células Epiteliales/efectos de los fármacos , Mucosa Bucal/efectos de los fármacos , Proteínas Ribosómicas/farmacología , Adolescente , Adulto , Proteínas Bacterianas/aislamiento & purificación , Adhesión Celular , Técnicas de Cocultivo , Células Epiteliales/citología , Células Epiteliales/inmunología , Escherichia coli/química , Femenino , Humanos , Inmunidad Innata , Lipopolisacáridos/aislamiento & purificación , Lipopolisacáridos/farmacología , Masculino , Mucosa Bucal/citología , Mucosa Bucal/inmunología , Cultivo Primario de Células , Proteínas Ribosómicas/aislamiento & purificación , Staphylococcus aureus/químicaRESUMEN
Definition of the biofilm process as one of the types of intercellular bacterial communications is presented. The modern data concerning the structure of the Pseudomonas aeruginosa biofilm matrix and genetic mechanisms necessary for its production are described. Active and passive rejections of biofilm bacteria, which are the basis of bacterial spreading to new surfaces, are discussed. The complexity and chain type of the reactions associated with biofilm formation are emphasized.
Asunto(s)
Biopelículas/crecimiento & desarrollo , Matriz Extracelular/microbiología , Genes Bacterianos/fisiología , Pseudomonas aeruginosa/crecimiento & desarrollo , Pseudomonas aeruginosa/genética , Adhesión Bacteriana , ADN Bacteriano/fisiología , Matriz Extracelular/genética , Lectinas/fisiología , Polisacáridos Bacterianos/fisiologíaRESUMEN
Natural biofilms rarely exist as monocultures. Usually they are formed from various microorganism species that interact with each other, have shared metabolites, strengthen the attachment of each other to the support substrate, provide expression of "foreign" genes etc. Material on factors and mechanisms that determine the formation of mixed (polymicrobial) biofilms is analyzed in the review. The significance of interspecies interaction between bacteria based on QS system signal autoinductors is underlined. Examples of humoral and contact communications between bacteria and eukaryotes including host cells are provided. Study of polymicrobial processes and their interaction with innate and adaptive immune response seems important for further development of medical microbiology (especially regarding chronic infectious diseases).