RESUMEN

While the application of enzymes to synthetic and industrial problems continues to grow, the major development today is focused on multi-enzymatic cascades. Such systems are particularly attractive, because many commercially available enzymes operate under relatively similar operating conditions. This opens the possibility of one-pot operation with multiple enzymes in a single reactor. In this paper the concept of modules is introduced whereby groups of enzymes are combined in modules, each operating in a single reactor, but with the option of various operating strategies to avoid any complications of nonproductive interactions between the enzymes, substrates or products in a given reactor. In this paper the selection of modules is illustrated using the synthesis of the bulk chemical, gluconic acid, from lignocellulosic waste.

Asunto(s)

Catalasa/química , Celulasas/química , Gluconatos/síntesis química , Glucosa Oxidasa/química , Lignina/química , Modelos Estadísticos , beta-Glucosidasa/química , Biocatálisis , Catalasa/metabolismo , Celulasas/metabolismo , Simulación por Computador , Fermentación , Gluconatos/química , Gluconatos/metabolismo , Glucosa/química , Glucosa/metabolismo , Glucosa Oxidasa/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Cinética , Lactonas/química , Lactonas/metabolismo , Lignina/metabolismo , Ingeniería Metabólica/métodos , Temperatura , Residuos , beta-Glucosidasa/metabolismo