RESUMEN
During a survey of ophiostomatoid fungi in native forests of southern Argentina, several isolates of Huntiella species were obtained from Nothofagus trees. Sequences of multiple gene regions were used to identify these fungi, and their pathogenicity was tested on N. pumilio and N. dombeyi. Phylogenetic analyses revealed a novel taxon described here as H. decorticans sp. nov. Inoculations on N. dombeyi and N. pumilio in the forest showed that H. decorticans is able to produce localized lesions on healthy Nothofagus trees.
Asunto(s)
Ascomicetos/aislamiento & purificación , Magnoliopsida/microbiología , Argentina , Ascomicetos/clasificación , Ascomicetos/genética , Ascomicetos/crecimiento & desarrollo , Datos de Secuencia Molecular , Filogenia , Enfermedades de las Plantas/microbiología , Esporas Fúngicas/clasificación , Esporas Fúngicas/genética , Esporas Fúngicas/crecimiento & desarrollo , Esporas Fúngicas/aislamiento & purificaciónRESUMEN
In a total of 165 blood samples from horses in the Province of Formosa (Argentina), the diagnosis for equine trypanosomiasis (T. evansi) was made using Giemsa-stained smears (GSS), wet blood films (WBF), Strout's concentration method (SCM), haematocrit centrifuge technique (HCT), buffy coat method (BCM) and mouse inoculation of blood (MBI). Trypanosoma evansi was demonstrated in 52 samples. Mouse inoculation gave a sensitivity of 88.2%; HCT 71.1%; BCM 63.4%; WBF 53.8%; SCM 46.1% and GSS 45.6%. No single method alone was totally effective. The haematocrit centrifuge technique, mouse inoculation of blood and Giemsa-stained smears were proposed as the most effective diagnostic combination.
Asunto(s)
Enfermedades de los Caballos/diagnóstico , Tripanosomiasis/veterinaria , Animales , Argentina , Bioensayo , Hematócrito/veterinaria , Enfermedades de los Caballos/sangre , Caballos , Ratones , Valor Predictivo de las Pruebas , Ratas , Trypanosoma/aislamiento & purificación , Tripanosomiasis/sangre , Tripanosomiasis/diagnósticoRESUMEN
While from the nature of things it is impossible to prove that patients whose lesions are of the bacteriologically negative, resistant type are under no circumstances able to trasmit leprosy, available evidence is against their being a source of danger to children, who are usually highly susceptible. With regard to the juvenile type, the frequency with which such cases suddenly develop widespread, bacteriologically positive lesions makes clear the necessity of keeping children suffering from this type of the disease under close observation, remembering the danger of their suddenly becoming actively infectious cases. THere is a tendency for advanced cutaneous-type cases to develop nerve lesions in the later stages of the disease. Patients seen at this stage are often classified as neural (properly, " secondary neural"). Routine bacteriological examination may at first fail to show Mycobacterium leprae, but repeated examination will often show clumps of acid-fast bacilli in the skin, nose or gums. Patients who have at one time suffered from widespread sutaneous leprosy with bacteriologically positive lesions should, even after the disease has become quiescent and arrested, be kept from close contact with healthy people and especially with children. Such contact should be permitted only when the disease has remained arrested for several years, when the patients have shown no signs of the disease on being subjected to the iodice test, and when all parts of the skin, nasal mucosa, gums, etc., have been carefully examined for bacteria and found negative. Reference is made to the use of the leprolin test in determining resistance to invasion by Myco. leprae and in judging the degree of danger of transmission of disease.
Asunto(s)
Humanos , Lepra Tuberculoide/clasificación , Lepra Tuberculoide/complicaciones , Lepra Tuberculoide/diagnóstico , Lepra Tuberculoide/terapia , Lepra Tuberculoide/transmisiónRESUMEN
The usefulness of a means for representing in a concise graphic form the progress of leprosy in individual cases over a long period of time is discussed, and a form for the purpose, based on the Conference classification, is presented. In it provision is also made for summarizing other outstanding facts, including the bacteriological findings, weight, concurrent clinical events, and treatment. Hyphothetical cases are presented in outline to illustrate the use of the chart, and a question arising in connection with recording neural case is discussed.