RESUMEN
This study was carried out to determine the relationships between oxidant/antioxidant status, in vitro LDL oxidizability and LDL-fatty acid composition in diabetes mellitus. Plasma total antioxidant capacity (oxygen radical absorbance capacity, ORAC) and LDL-cholesteryl ester fatty acids were investigated in type 1 and type 2 diabetic subjects with and without complications. The degree of LDL oxidation was determined by the measurement of hydroperoxide levels before and after in vitro peroxidative stress with CuSO4. ORAC values were decreased in diabetic subjects who showed high basal hydroperoxide levels. Oxidizability of LDL in these subjects was higher than in control subjects and it was unrelated to LDL-fatty acid composition. However, in type 2 diabetic subjects with complications, alterations in LDL-fatty acid composition were associated with their enhanced oxidative susceptibility. LDL-fatty acid alterations might be an additional factor that influences LDL oxidizability especially in type 2 diabetes. In conclusion, diabetes mellitus is associated with enhanced oxidative stress and defective antioxidant/oxidant balance regardless the type of diabetes and presence of complications.
Asunto(s)
Antioxidantes/metabolismo , Diabetes Mellitus Tipo 1/sangre , Diabetes Mellitus Tipo 2/sangre , Susceptibilidad a Enfermedades/sangre , Ácidos Grasos/sangre , Lipoproteínas LDL/sangre , Oxidantes/sangre , Adulto , Femenino , Humanos , Peroxidación de Lípido , Masculino , Persona de Mediana Edad , Oxidación-Reducción , Estrés OxidativoRESUMEN
A 56-kDa polypeptide suspected to be the tanning hormone 'bursicon' was analyzed using the monoclonal antibody (mAb) 01C10 of Song and Ma. We studied the beetle Tenebrio molitor, for which data on bursicon have been recently published. After purification by two-dimensional gel electrophoresis of brain proteins, the immunoreactive 56-kDa polypeptide was trypsinated and microsequenced. The obtained sequences revealed a high homology with alpha- and beta-tubulins. In a complementary study, immunoreactive clones were isolated, using the 01C10 mAb, from a library in expression vector obtained from Drosophila melanogaster head cDNAs. Again, the isolated clones were found, after cDNA sequencing, to correspond to tubulin. Our results suggest that, although the 01C10 mAb could possibly still have a great affinity for a polypeptide present in very low quantities in a few brain neurosecretory cells, it also proved to have an artefactual affinity for a 56-kDa polypeptide, identified as tubulin, which is not involved in tanning control.
Asunto(s)
Hormonas de Invertebrados/inmunología , Tenebrio/fisiología , Tubulina (Proteína)/inmunología , Animales , Anticuerpos Monoclonales , Afinidad de Anticuerpos , Western Blotting , Encéfalo/metabolismo , Células Cultivadas , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Drosophila/genética , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Biblioteca de Genes , Hormonas de Invertebrados/fisiología , Análisis de Secuencia de ADN , Factores de Tiempo , Tubulina (Proteína)/metabolismo , Tubulina (Proteína)/fisiologíaRESUMEN
After the metamorphosis of Tenebrio molitor (Coleoptera), Acetylcholinesterase (AChE) activity per adult brain was twofold the activity of the last larval instar. During metamorphosis, from the prepupa to the adult fluctuations occurred in brain AChE activity per protein amount. These fluctuations were superimposable to the molting hormone (ecdysterone) variations in hemolymph so that each rise of ecdysterone was accompanied by an AChE peak.
Asunto(s)
Acetilcolinesterasa/metabolismo , Ecdisterona/metabolismo , Metamorfosis Biológica , Tenebrio/crecimiento & desarrollo , Animales , Encéfalo/enzimología , Encéfalo/crecimiento & desarrolloRESUMEN
During the 9-day pupal period of Tenebrio molitor (the mealworm beetle), the cells of the male accessory glands undergo divisions for 7 days. There are two maxima in the mitotic activity in the glands in vivo, one at 1 day and the other at 4 days. The latter peak coincides with the large surge of ecdysterone occurring in the pupal stage. By the use of in vitro culture techniques, it has been demonstrated that the first bout of mitosis in both glands proceeds in basal medium, while the second bout of mitosis requires a physiological level of ecdysterone. Ecdysone was less effective than ecdysterone. Sensitivity to ecdysterone did not change significantly between Day 1 and Day 4 of the pupal stage. The results are discussed in relation to the effects of ecdysterone on cell division in mesodermal and ectodermal derivatives.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , Ecdisterona/farmacología , Mitosis/efectos de los fármacos , Tenebrio/crecimiento & desarrollo , Factores de Edad , Animales , Ecdisona/farmacología , Masculino , Microscopía Electrónica de Rastreo , Técnicas de Cultivo de Órganos , Pupa/anatomía & histología , Pupa/citología , Tenebrio/citologíaRESUMEN
The leg of the last instar larva, and especially the pharate pupa, of Tenebrio molitor (Coleoptera) shows considerable restoration ability. Restoration refers to the regeneration of leg structures removed during the last larval instar. This process involves only those tissues adjacent to the cut surface. At each level of the leg, amputation before a characteristic time leads to the restoration of a complete leg, but thereafter the new segments formed are hypomorphic or just lobes. After an amputation close to the pupal ecdysis, fragments are obtained, and these incomplete structures show that the restoration process has ceased. Just before the pupal ecdysis the leg is withdrawn from the cuticle, so a very late amputation does not effect the leg tissue and a complete pupal and adult leg is formed. The whole of the larval leg participates in adult leg morphogenesis, and histological techniques demonstrate a great correspondence between larval and adult leg segments. The larval trochanter, however, forms the adult trochanter and the proximal part of the femur. The larval tibia forms the adult tibia plus tarsus, except for the adult claws which develop from the larval tarsal claw.