RESUMEN
Suppressors of cytokine signalling (SOCS) molecules inhibit cytokine signalling and may regulate protective immunity in tuberculosis (TB). We investigated the association of SOCS with disease progression in patients with pulmonary TB. For this purpose, we studied peripheral blood mononuclear cells (PBMCs) and T cells from patients with pulmonary TB (TB, n=33) and healthy endemic controls (EC, n=15). Cases were stratified into those with moderately advanced (Mod-PTB) or far advanced disease (Adv-PTB). Interferon-gamma (IFN-γ), SOCS1 and SOCS3 gene expression was determined by RT-PCR. Statistical analysis was performed using the Mann-Whitney test. Levels of IL6 (P=0.018) and IL10 (P=0.013) were found to be elevated in PBMC supernatants from patients with TB as compared with EC. SOCS1 mRNA gene expression in T cells from patients with TB was increased as compared with that of EC (P=0.02). In addition, levels of SOCS1 mRNA transcripts were found to be elevated in PBMCs of Adv-PTB as compared with Mod-PTB (P=0.008) cases. Our data show that raised SOCS1 levels are associated with increased disease severity in TB. As SOCS1 regulates IFN-γ-driven immunity and SOCS1 can be further upregulated by IL6 levels, the increase in SOCS1 in severe disease indicates a mechanism by which mycobacteria impede disease control in TB.
Asunto(s)
Interferón gamma/genética , Leucocitos Mononucleares/inmunología , Mycobacterium tuberculosis/inmunología , Proteínas Supresoras de la Señalización de Citocinas/genética , Tuberculosis Pulmonar/genética , Adolescente , Adulto , Anciano , Femenino , Expresión Génica , Interacciones Huésped-Patógeno , Humanos , Interferón gamma/inmunología , Interleucina-10/biosíntesis , Interleucina-10/inmunología , Interleucina-6/biosíntesis , Interleucina-6/inmunología , Leucocitos Mononucleares/microbiología , Leucocitos Mononucleares/patología , Masculino , Persona de Mediana Edad , ARN Mensajero/biosíntesis , ARN Mensajero/inmunología , Índice de Severidad de la Enfermedad , Proteína 1 Supresora de la Señalización de Citocinas , Proteína 3 Supresora de la Señalización de Citocinas , Proteínas Supresoras de la Señalización de Citocinas/inmunología , Tuberculosis Pulmonar/inmunología , Tuberculosis Pulmonar/microbiologíaRESUMEN
Improved tools are required to study immunopathogenesis of tuberculosis (TB). Mycobacterium tuberculosis antigen-stimulated T cell-based assays can detect TB but are less effective when responses are compromised such as in severe disease. We investigated immune responses to M. tuberculosis whole sonicate (MTBs), recombinant antigens ESAT6 and CFP10 in whole blood cells of healthy endemic controls (EC, n = 42) and patients with pulmonary (PTB, n = 36) or extrapulmonary (ETB, n = 41) disease. Biomarkers of T cell activation (IFNγ) or modulation (IL10) and chemokines, CXCL9, CXCL10 and CCL2, secretion were measured. MTBs, ESAT6 and CFP10 all induced IFNγ responses in TB. ESAT6-induced IFNγ was elevated in TB as compared with EC. MTBs stimulated the highest IFNγ levels but did not differentiate between TB and EC. However, MTBs-induced CXCL10 (P = 0.004) was reduced, while IL10 (P < 0.001) was raised in TB as compared with EC. Between sites, MTBs-induced CCL2 (P = 0.001) and IL10 secretion was higher in PTB than ETB (P < 0.001). In comparison of disease severity, MTBs-induced IFNγ (P = 0.014) and CXCL10 (P = 0.022) levels were raised in moderate as compared with far advanced PTB. In ETB, MTBs-induced IL10 levels were greater in less-severe (L-ETB) than in severe disseminated (D-ETB) cases, P = 0.035. Within the L-ETB group, MTBs-induced IFNγ was greater in patients with tuberculous lymphadenitis than those with pleural TB (P = 0.002). As immune responses to MTBs were differentially activated in TB of different sites and severity, we propose the utility of MTBs-induced IFNγ, CXCL10 and IL10 as biomarkers in TB.
Asunto(s)
Quimiocina CXCL10/metabolismo , Interferón gamma/metabolismo , Interleucina-10/metabolismo , Mycobacterium tuberculosis/química , Tuberculosis Ganglionar/diagnóstico , Tuberculosis Pulmonar/diagnóstico , Biomarcadores/metabolismo , Células Sanguíneas/efectos de los fármacos , Células Sanguíneas/inmunología , Células Sanguíneas/microbiología , Estudios de Casos y Controles , Quimiocina CXCL10/inmunología , Mezclas Complejas/inmunología , Mezclas Complejas/farmacología , Humanos , Interferón gamma/inmunología , Interleucina-10/inmunología , Mycobacterium tuberculosis/inmunología , Mycobacterium tuberculosis/aislamiento & purificación , Cultivo Primario de Células , Índice de Severidad de la Enfermedad , Sonicación , Tuberculosis Ganglionar/inmunología , Tuberculosis Ganglionar/microbiología , Tuberculosis Ganglionar/patología , Tuberculosis Pulmonar/inmunología , Tuberculosis Pulmonar/microbiología , Tuberculosis Pulmonar/patologíaRESUMEN
Trypanosoma brucei subspecies invade the brain parenchyma at late stages of human and experimental rodent infections. In this study, we compared the outcome of infection with T. b. brucei in MHC-matched (H-2b) C57BL/6 (B6) and 129Sv/Ev (Sv-129). Sv-129 showed higher parasitaemia and lower specific IgM (but not IgG) antibody levels than B6 mice. The number of trypanosomes, CD4+ and CD8+ T cells in the brain parenchyma was higher in B6 mice. B6 mice lost weight and showed higher cumulative mortality when compared with Sv-129 mice. Higher levels of IL-1beta, IL-6, IL-10, TNF-alpha, IFN-gamma, ICAM-1 and E-selectin, but low levels of TGF-beta mRNA were present in brains of B6 when compared with Sv-129-infected mice. Thus, host genetics differentially determine the invasion of T. b. brucei into the brain parenchyma, which is paralleled by the severity of inflammation in the brain and course of the disease, but not by parasitaemia nor by antibody titres.
Asunto(s)
Encefalopatías/inmunología , Encefalopatías/parasitología , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/inmunología , Trypanosoma brucei brucei/inmunología , Tripanosomiasis Africana/inmunología , Animales , Anticuerpos Antiprotozoarios/sangre , Moléculas de Adhesión Celular/biosíntesis , Moléculas de Adhesión Celular/genética , Citocinas/biosíntesis , Citocinas/genética , Proteína Ácida Fibrilar de la Glía , Antígenos de Histocompatibilidad/inmunología , Interacciones Huésped-Patógeno , Inmunohistoquímica , Antígeno de Macrófago-1/biosíntesis , Antígeno de Macrófago-1/genética , Ratones , Ratones Endogámicos C57BL , Proteínas del Tejido Nervioso/biosíntesis , Proteínas del Tejido Nervioso/genética , Parasitemia/inmunología , Parasitemia/parasitología , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Tripanosomiasis Africana/genética , Tripanosomiasis Africana/parasitologíaRESUMEN
Neurons can be targets for microbes, which could kill the neurons. Just in reverse, we, in this study, report that bacteria can be killed when entering a neuron. Primary cultures of foetal mouse hippocampal neurons and a neuronal cell line derived from mouse hypothalamus were infected by Listeria monocytogenes. Treatment with interferon-gamma (IFN-gamma) did not affect bacterial uptake, but resulted in increased killing of intracellular bacteria, whereas the neuronal cell remained intact. The IFN-gamma-mediated bacterial killing was mapped to the neuronal cytosol, before listerial actin tail formation. Treatment with IFN-gamma induced phosphorylation of the transcription factor STAT-1 in neurons and IFN-gamma-mediated listerial killing was not observed in STAT-1(-/-) neurons or neurons treated with IFN regulatory factor-1 antisense oligonucleotides. IFN-gamma-treated neuronal cells showed increased levels of inducible nitric oxide synthase (iNOS) mRNA, and antisense iNOS oligonucleotides hampered the bacterial killing by neurons upon IFN-gamma treatment. This novel neuronal function - i.e., that of a microbe killer - could play a crucial role in the control of infections in the immuno-privileged nervous system.
Asunto(s)
Antivirales/farmacología , Interferón gamma/farmacología , Listeriosis/metabolismo , Neuronas/metabolismo , Animales , Proteínas de Unión al ADN/metabolismo , Factor 1 Regulador del Interferón , Listeriosis/tratamiento farmacológico , Listeriosis/inmunología , Ratones , Neuronas/efectos de los fármacos , Neuronas/inmunología , Neuronas/microbiología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óxido Nítrico Sintasa de Tipo II , Fosfoproteínas/metabolismo , Factor de Transcripción STAT1 , Factores de Tiempo , Transactivadores/metabolismo , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Immunization with different adjuvants resulted in antithetic outcomes of infection with Chlamydia pneumoniae. Immunization with the outer major protein-2 from C. pneumoniae (OMP-2) emulsified in Freund's complete adjuvant (FCA) thus increased the susceptibility of mice to infection with the bacteria. The detrimental effect was not observed upon inoculation of irrelevant antigens or major outer membrane protein (MOMP) in FCA, but was also observed after immunization with FCA-chlamydial heat shock protein-60 (HSP-60). The harmful effect of FCA-OMP-2 depended on the presence of both CD4+ and CD8+ cells and was mediated by IL-10, as shown using gene-ablated mice. The increased susceptibility to infection caused by FCA-OMP-2 immunization was long-lasting and observed in mice infected 4 months after the last dose of immunogen. In contrast, partial protection against C. pneumoniae was observed when FCA was replaced with oligodeoxynucleotides containing immunostimulatory CpG motifs mixed with Freund's incomplete adjuvant (FIA-IS-CpG). These polar outcomes of infection related to the cytokine pattern: antigen-stimulated spleen cells from FCA-OMP-2-immunized mice showed higher IL-10/IFN-gamma ratios than FIA-IS-CpG-OMP-2-immunized animals. In agreement, sera from FCA-OMP-2 showed higher anti-OMP-2 IgG1/IgG2a ratios than FIA-IS-CpG-OMP-2-immunized animals. Finally, OMP-2 also generated a protective response when delivered by a eukaryotic expression vector in tandem with CTLA4, a procedure that targeted OMP-2 to antigen-presenting cells.
Asunto(s)
Adyuvantes Inmunológicos/administración & dosificación , Vacunas Bacterianas/administración & dosificación , Infecciones por Chlamydophila/prevención & control , Chlamydophila pneumoniae/inmunología , Pulmón/inmunología , Neumonía Bacteriana/inmunología , Animales , Anticuerpos/inmunología , Proteínas de la Membrana Bacteriana Externa/genética , Chaperonina 60/administración & dosificación , Infecciones por Chlamydophila/inmunología , Citocinas/inmunología , Ratones , Ratones Endogámicos C57BL , Células TH1/inmunología , Vacunas de ADN/administración & dosificaciónRESUMEN
Several reports have indicated that cell lineages apart from NK and T cells can also express IFN-gamma. However, the biological relevance of this finding is uncertain. We show in this study that bone marrow-derived macrophages (BMMs) express IFN-gamma at the mRNA and protein level early after infection with Chlamydia pneumoniae. Increased IFN-gamma mRNA accumulation by infected BMMs is early, transient, and requires both bacterial and host protein synthesis. The induction of IFN-gamma mRNA levels is independent of IL-12 and was dramatically enhanced in IL-10(-/-) BMMs. Such IL-10(-/-) BMMs contained less bacteria than the wild-type controls, whereas IFN-gammaR(-/-) BMMs showed increased C. pneumoniae load. Inducible NO synthase (iNOS) also participates in the control of bacterial load, as shown by the enhanced numbers of C. pneumoniae in iNOS(-/-) BMMs. However, the increased accumulation of iNOS mRNA and NO in C. pneumoniae-infected BMMs depended on the presence of IFN-alphabeta, but was independent of IFN-gamma. Interestingly, IFN-alphabeta are also required for increased IFN-gamma mRNA accumulation in C. pneumoniae-infected BMMs. Accordingly, IFN-alphabetaR(-/-) BMMs showed higher levels of C. pneumoniae than wild-type BMMs. Our findings unravel an autocrine/paracrine macrophage activation pathway by showing an IFN-alphabeta-dependent IFN-gamma and iNOS induction in response to infection, which protects macrophages against intracellular bacterial growth.
Asunto(s)
Células de la Médula Ósea/inmunología , Células de la Médula Ósea/microbiología , Chlamydophila pneumoniae/crecimiento & desarrollo , Chlamydophila pneumoniae/inmunología , Interferón Tipo I/fisiología , Interferón gamma/metabolismo , Macrófagos/inmunología , Macrófagos/microbiología , Animales , Antibacterianos/metabolismo , Antibacterianos/farmacología , Células de la Médula Ósea/enzimología , Células de la Médula Ósea/metabolismo , Células Cultivadas , Inhibidores de Crecimiento/metabolismo , Inhibidores de Crecimiento/fisiología , Interferón gamma/biosíntesis , Interferón gamma/genética , Interleucina-10/fisiología , Interleucina-12/fisiología , Líquido Intracelular/enzimología , Líquido Intracelular/inmunología , Líquido Intracelular/microbiología , Macrófagos/enzimología , Macrófagos/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones Transgénicos , Óxido Nítrico Sintasa/biosíntesis , Óxido Nítrico Sintasa de Tipo II , ARN Mensajero/biosíntesis , ARN Mensajero/genéticaRESUMEN
The pathologic features of cerebral Listeria monocytogenes infection strongly suggest that besides hematogenous spread, bacteria might also spread via a neural route. We propose that after snout infection of recombination activating gene 1 (RAG-1)-deficient mice, L. monocytogenes spreads to the brain via a neural route. The neural route of invasion is suggested by (i) the immunostaining of L. monocytogenes in the trigeminal ganglia (TG) and brain stem but not in other areas of the brain; (ii) the kinetics of bacterial loads in snout, TG, and brain; and (iii) the increased resistance of mice infected with a plcB bacterial mutant (unable to spread from cell to cell). Gamma interferon (IFN-gamma) plays a protective role in neuroinvasion; inducible nitric oxide synthase (iNOS) accounts only partially for the protection, as shown by a comparison of the susceptibilities of IFN-gamma receptor (IFN-gamma R)-deficient, iNOS-deficient, and wild-type mice to snout infection with L. monocytogenes. The dramatically enhanced susceptibility of RAG-1-deficient, IFN-gamma R gene-deficient mice indicated the overall importance of innate immune cells in the release of protective levels of IFN-gamma. The source of IFN-gamma appeared to be NK cells, as shown by use of RAG-1-deficient, gamma-chain receptor gene-deficient mice; NK cells played a relevant protective role in neuroinvasion through a perforin-independent mechanism. In vitro evidence indicated that IFN-gamma can directly induce bacteriostatic mechanisms in neural tissue.
Asunto(s)
Encéfalo/microbiología , Listeria monocytogenes/inmunología , Animales , Citotoxicidad Inmunológica , Interferón gamma/fisiología , Células Asesinas Naturales/inmunología , Ratones , Ratones Endogámicos C57BL , Óxido Nítrico Sintasa/fisiología , Óxido Nítrico Sintasa de Tipo II , Fosfolipasas/fisiología , Receptores de Interferón/fisiología , Ganglio del Trigémino/microbiología , Receptor de Interferón gammaAsunto(s)
Anestésicos por Inhalación/administración & dosificación , Infecciones por Chlamydia/veterinaria , Chlamydophila pneumoniae/patogenicidad , Metoxiflurano/administración & dosificación , Ratones Endogámicos BALB C/fisiología , Enfermedades de los Roedores/fisiopatología , Administración Intranasal , Animales , Anticuerpos Antibacterianos/química , Dióxido de Carbono/administración & dosificación , Infecciones por Chlamydia/fisiopatología , Chlorocebus aethiops , Modelos Animales de Enfermedad , Femenino , Pulmón/microbiología , Ratones , Organismos Libres de Patógenos Específicos , Estadísticas no Paramétricas , Células VeroRESUMEN
By using mice genomically lacking IFN-gammaR, IL-12, perforin, and recombination-activating gene-1 (RAG-1), we analyzed the regulation and importance of IFN-gamma in the control of infection with Chlamydia pneumoniae. IL-12 participates in resistance of mice to C. pneumoniae, probably by regulating the protective levels of IFN-gamma mRNA. In turn, IFN-gamma is necessary for the increased IL-12p40 mRNA accumulation that occurs in lungs during infection with C. pneumoniae, suggesting a positive feedback regulation between these two cytokines. In experiments including RAG-1-/-/IFN-gammaR-/- mice we showed that IFN-gamma produced by innate cells controls the bacterial load and is necessary for the increased accumulation of transcripts for enzymes controlling high output NO release (inducible NO synthase), superoxide production (gp-91 NADPH oxidase), and catalysis of tryptophan (indoleamine 2, 3-dioxygenase (IDO)), mechanisms probably related to bacterial killing. Adaptive immune responses diminish the levels of IFN-gamma and IL-12 mRNA and thereby the levels of inducible NO synthase, IDO, and gp91 NADPH oxidase transcripts. By using RAG-1-/-/perforin-/- mice, we excluded the overt participation of NK cell cytotoxicity in the control of C. pneumoniae. However, NK cells and probably other innate immune cells release IFN-gamma during the bacterial infection.
Asunto(s)
Infecciones por Chlamydia/genética , Infecciones por Chlamydia/inmunología , Chlamydophila pneumoniae/inmunología , Interferón gamma/biosíntesis , Interferón gamma/fisiología , Animales , Infecciones por Chlamydia/prevención & control , Citotoxicidad Inmunológica/genética , Gangliósido G(M1)/inmunología , Genes RAG-1/inmunología , Predisposición Genética a la Enfermedad , Sueros Inmunes/administración & dosificación , Inmunidad Innata/genética , Inmunosupresores/administración & dosificación , Inyecciones Intravenosas , Interferón gamma/genética , Interferón gamma/metabolismo , Células Asesinas Naturales/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Receptores de Interferón/genética , Eliminación de Secuencia , Receptor de Interferón gammaRESUMEN
We have investigated the efficacy of the DNA vaccination using the heat shock protein 60 (HSP-60) gene of C. pneumoniae, for protection of mice against infection with the bacteria. C57Bl/6 mice had a 5-20-fold reduction of C. pneumoniae numbers in lungs when immunized intranasally (i.n.) with plasmids (p) encoding pHSP-60. The reduction of the bacterial load coincided with a decreased severity of disease. No specific antibodies were detected after protective i. n. immunization. In contrast, mice immunized intradermally (i.d.) were not protected against challenge with C. pneumoniae, although specific humoral Immunoglobulin (Ig)G responses were generated. Co-inoculation i.n. of pHSP-60 with pIL-12 but not with pGM-CSF further increased protection of mice against infection with C. pneumoniae. Lungs from pHSP-60 i.n. immunized and infected mice showed higher levels of interferon (IFN)-gamma mRNA, and spleen cells from these mice co-cultured with r-HSP-60 released higher levels of IFN-gamma and displayed higher proliferative responses than nonimmunized and infected controls. pHSP-60 immunized IFN-gamma receptor (R)-/- mice were not protected against infection with C. pneumoniae. Likewise, i.n. administration of pIFN-gamma alone induced significant protection. DNA vaccine-induced protection was CD4+ and CD8+ T-cell dependent, as shown by DNA-vaccination of MHC class II-/-, CD4-/-, CD8-/- and CD4-/-CD8-/-mice. Interestingly, DNA vaccine induced CD4+ T cells, in the absence of CD8+ T cells, were involved in worsening the outcome of infection. This worsening was linked with a shift towards a Th2 cytokine pattern.
Asunto(s)
Chaperonina 60/inmunología , Infecciones por Chlamydia/prevención & control , Chlamydophila pneumoniae/inmunología , Inmunización , Neumonía Bacteriana/prevención & control , Vacunas de ADN/administración & dosificación , Animales , Anticuerpos Antibacterianos/análisis , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/inmunología , Chaperonina 60/genética , Infecciones por Chlamydia/inmunología , Infecciones por Chlamydia/patología , Chlamydophila pneumoniae/genética , Chlamydophila pneumoniae/aislamiento & purificación , Recuento de Colonia Microbiana , Femenino , Inmunoglobulina G/análisis , Interferón gamma/administración & dosificación , Interferón gamma/genética , Interleucina-12/administración & dosificación , Pulmón/microbiología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Mutantes , Mucosa Nasal/inmunología , Plásmidos , Neumonía Bacteriana/inmunología , Neumonía Bacteriana/patología , ARN Mensajero/metabolismoRESUMEN
The interferon-gamma receptor gene was detected in the rat hypothalamic suprachiasmatic nuclei (SCN), the main pacemaker for circadian rhythms, and the molecular identity of the transcript was confirmed by sequencing. The expression of the receptor protein showed a daily rhythm that was dependent on light. It reached its adult pattern in the SCN between postnatal day 11 and 20, i.e., at a time when capacity for photic entrainment of the pacemaker is established.
Asunto(s)
Envejecimiento/inmunología , Relojes Biológicos/fisiología , Ritmo Circadiano/fisiología , Regulación del Desarrollo de la Expresión Génica/efectos de la radiación , Receptores de Interferón/genética , Núcleo Supraquiasmático/inmunología , Transcripción Genética , Animales , Masculino , Ratas , Ratas Sprague-Dawley , Núcleo Supraquiasmático/crecimiento & desarrollo , Núcleo Supraquiasmático/efectos de la radiación , Receptor de Interferón gammaRESUMEN
Infection with Chlamydia pneumoniae is a common cause of acute respiratory disease in man and is also associated with atherosclerotic cardiovascular disorder. Herein, we have compared bacterial load and immune parameters of C. pneumoniae-infected mice genomically lacking T cell coreceptors, cytokine receptors, or cytotoxic effector molecules. A protective role for CD8+ cells is shown by the enhanced severity of infection of CD8-/- or TAP-1-/-/beta2-microglobulin -/- mice. CD8+ cells hindered a parasite growth-promoting role of CD4+ T cells, as indicated by the higher sensitivity to early infection of CD8-/- than CD4-/-/CD8-/- mice, which was further confirmed in experiments in which SCID mice were reconstituted with either CD4+ or CD4+ plus CD8+ T cells. Interestingly, CD4+ T cells played a dual role, detrimental early (14 and 24 days) after infection but protective at later time points (60 days after infection). The CD8+ T cell protection was perforin independent. The early deleterious role of CD4+ in the absence of CD8+ T cells was associated with enhanced IL-4 and IL-10 mRNA levels and delayed IFN-gamma mRNA accumulation in lungs. In line with this, IFN-gammaR-/- (but not TNFRp55 -/-) mice showed dramatically increased susceptibility to C. pneumoniae, linked to reduced inducible nitric oxide synthase (iNOS) mRNA accumulation, but not to diminished levels of specific Abs. The increased susceptibility of iNOS-/- mice indicates a protective role for iNOS activity during infection with C. pneumoniae. The higher sensitivity of IFN-gammaR-/- mice to C. pneumoniae compared with that of SCID or recombination-activating gene-1-/- mice suggested a relevant protective role of IFN-gamma-dependent innate mechanisms of protection.
Asunto(s)
Infecciones por Chlamydia/inmunología , Chlamydophila pneumoniae/inmunología , Animales , Antígenos CD/fisiología , Linfocitos T CD4-Positivos/fisiología , Linfocitos T CD8-positivos/fisiología , Inmunidad , Interferón gamma/fisiología , Ratones , Ratones Endogámicos BALB C , Ratones SCID , Óxido Nítrico Sintasa/fisiología , Óxido Nítrico Sintasa de Tipo II , Receptores de Interferón/fisiología , Receptores del Factor de Necrosis Tumoral/fisiología , Receptores Tipo I de Factores de Necrosis Tumoral , Receptor de Interferón gammaRESUMEN
Tumor necrosis factor receptor p55 (TNFRp55) mediates host resistance to several pathogens by allowing microbicidal activities of phagocytes. In the studies reported here, TNFRp55-/- mice infected with the intracellular parasite Trypanosoma cruzi showed clearly higher parasitemia and cumulative mortality than wild-type (WT) controls did. However, gamma interferon (IFN-gamma)-activated macrophages from TNFRp55-/- mice produced control levels of nitric oxide and killed the parasite efficiently in vitro. Trypanocidal mechanisms of nonphagocytic cells (myocardial fibroblasts) from both TNFRp55-/- and WT mice were also activated by IFN-gamma in a dose-dependent way. However, IFN-gamma-activated TNFRp55-/- nonphagocytes showed less effective killing of T. cruzi than WT control nonphagocytes, even when interleukin 1beta (IL-1beta) was added as a costimulator. In vivo, T. cruzi-infected TNFRp55-/- mice and WT mice released similar levels of NO and showed similar levels of IFN-gamma mRNA and inducible nitric oxide synthase mRNA in their tissues. Instead, increased susceptibility to T. cruzi of TNFRp55-/- mice was associated with reduced levels of parasite-specific immunoglobulin G (IgG) (but not IgM) antibodies during infection, which is probably linked to abnormal B-cell differentiation in secondary lymphoid tissues of the mutant mice. Surprisingly, T. cruzi-infected TNFRp55-/- mice showed increased inflammatory and necrotic lesions in several tissues, especially in skeletal muscles, indicating that TNFRp55 plays an important role in controlling the inflammatory process. Accordingly, levels of Mn2+ superoxide dismutase mRNA, a TNF-induced enzyme which protects the cell from the toxic effects of superoxide, were lower in mutant than in WT infected mice.
Asunto(s)
Antígenos CD/metabolismo , Enfermedad de Chagas/inmunología , Parasitemia/inmunología , Receptores del Factor de Necrosis Tumoral/metabolismo , Animales , Anticuerpos Antiprotozoarios/sangre , Enfermedad de Chagas/mortalidad , Susceptibilidad a Enfermedades , Fibroblastos/inmunología , Fibroblastos/parasitología , Corazón/parasitología , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Interferón gamma/farmacología , Macrófagos Peritoneales/inmunología , Macrófagos Peritoneales/parasitología , Ratones , Ratones Mutantes , Miocardio/citología , Nitratos/sangre , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa/biosíntesis , Óxido Nítrico Sintasa de Tipo II , Parasitemia/mortalidad , Receptores Tipo I de Factores de Necrosis Tumoral , Bazo/patología , Superóxido Dismutasa/biosíntesis , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
The suprachiasmatic nuclei serve as the dominant circadian pacemaker in the mammalian brain, regulating daily behavioral, physiological and hormonal rhythms. In the ventrolateral parts of these nuclei, the receptor for the key immunoregulatory molecule interferon-gamma (IFN-gamma) was detected in the rat brain. The cellular localization of the IFN-gamma receptor corresponded to neuronal elements. Expression of the receptor followed a diurnal rhythm with a peak at zeitgeber time 15. This peak coincided with an enhanced expression of janus kinase 1 and 2 as well as the signal transducer and activator of transcription 1, which constitute the main intracellular signaling pathway of IFN-gamma. This is the first study to show expression of the receptor of an immune modulatory molecule in the pacemaker of the biological clock, which, thus, may be influenced by immune system signal molecules.
Asunto(s)
Ritmo Circadiano/fisiología , Interferón gamma , Receptores de Interferón/análisis , Núcleo Supraquiasmático/química , Animales , Inmunohistoquímica , Masculino , Oscilometría , Reacción en Cadena de la Polimerasa/métodos , Ratas , Ratas Sprague-Dawley , Transcripción GenéticaRESUMEN
The influence of ion composition, pH, and peptide concentration on the conformation and activity of the 37-residue human antibacterial peptide LL-37 has been studied. At micromolar concentration in water, LL-37 exhibits a circular dichroism spectrum consistent with a disordered structure. The addition of 15 mM HCO3-, SO42-, or CF3CO2- causes the peptide to adopt a helical structure, with approximately equal efficiency, while 160 mM Cl- is less efficient. A cooperative transition from disordered to helical structure is observed as the peptide concentration is increased, consistent with formation of an oligomer. The extent of alpha-helicity correlates with the antibacterial activity of LL-37 against both Gram-positive and Gram-negative bacteria. Two homologous peptides, FF-33 and SK-29, containing 4 and 8 residue deletions at the N terminus, respectively, require higher concentrations of anions for helix formation and are less active than LL-37 against Escherichia coli D21. Below pH 5, the helical content of LL-37 gradually decreases, and at pH 2 it is entirely disordered. In contrast, the helical structure is retained at pH over 13. The minimal inhibitory concentration of LL-37 against E. coli is 5 microM, and at 13-25 microM the peptide is cytotoxic against several eukaryotic cells. In solutions containing the ion compositions of plasma, intracellular fluid, or interstitial fluid, LL-37 is helical, and hence it could pose a danger to human cells upon release. However, in the presence of human serum, the antibacterial and the cytotoxic activities of LL-37 are inhibited.
Asunto(s)
Antibacterianos/metabolismo , Péptidos Catiónicos Antimicrobianos , Proteínas Portadoras/metabolismo , Animales , Aniones , Antibacterianos/química , Actividad Bactericida de la Sangre , Proteínas Portadoras/química , Catelicidinas , Escherichia coli , Humanos , Concentración de Iones de Hidrógeno , Estructura Secundaria de Proteína , Relación Estructura-Actividad , PorcinosRESUMEN
Evidence is presented from studies in vitro and in vivo for a dual pathway of inducible nitric oxide synthase (iNOS) induction during Trypanosoma cruzi infection, one of which is interferon (IFN)-gamma dependent and the other not. In vitro, the IFN-gamma-dependent iNOS induction decreases parasite multiplication, and is in vivo associated with protection. iNOS induced by this pathway mediated a high NO output and showed a diffuse, cytoplasmic immunostaining in IFN-gamma-activated macrophages in vitro as well as in cell infiltrates or infected tissues. Surprisingly, in such tissues, iNOS co-localized with parasite nests, and by immunoelectromicroscopy, iNOS was demonstrated on the parasite surface. iNOS co-localization with parasites was also seen in tissues from T. cruzi-infected IFN-gamma receptor (R) knockout mice suggesting an IFN-gamma-independent pathway of induction. However, no cytoplasmic iNOS was seen in inflammatory infiltrates of these tissues. IFN-gammaR(-/-) mice displayed a dramatically enhanced susceptibility to infection with T. cruzi, diminished accumulation of iNOS mRNA in skeletal muscle and spleen cells, and reduced release of NO and peroxynitrite. Expression of iNOS around intracellular parasites was also observed after infection of peritoneal macrophages or L-929 fibroblasts in vitro in the absence of other exogenous stimuli. A time-dependent NO release and enhanced accumulation of iNOS mRNA also was observed in infected peritoneal cells and fibroblasts. Cultured T. cruzi amastigotes, trypomastigotes, and epimastigotes were not labeled by the anti-iNOS antibodies and contained no iNOS mRNA, indicating that the iNOS detected actually originated from the mammalian cell. A pathogenic effect of low NO levels is suggested by the arresting effect of NOS inhibitors and the enhancing consequences of low concentrations of NO donors on intracellular parasite multiplication.
Asunto(s)
Óxido Nítrico Sintasa/biosíntesis , Óxido Nítrico Sintasa/inmunología , Trypanosoma cruzi/inmunología , Animales , Enfermedad de Chagas/enzimología , Enfermedad de Chagas/inmunología , Enfermedad de Chagas/parasitología , Reacciones Cruzadas , Inducción Enzimática/efectos de los fármacos , Interferón gamma/deficiencia , Interferón gamma/genética , Interferón gamma/farmacología , Células L , Ratones , Ratones Noqueados , Músculo Esquelético/inmunología , Músculo Esquelético/parasitología , Músculo Esquelético/ultraestructura , Óxido Nítrico Sintasa/ultraestructura , Bazo/inmunología , Bazo/parasitología , Bazo/ultraestructura , Trypanosoma cruzi/ultraestructuraRESUMEN
Formation of inflammatory lesions, one of the pathologic consequences of infection with Trypanosoma cruzi, involves intricate cell-cell interactions in which cell adhesion molecules (CAMs) are involved. Sera from 56 Chagas' disease patients grouped according to disease severity were studied for the presence of soluble intercellular adhesion molecule-1 (s-ICAM-1), soluble endothelial selectin (s-E-selectin), soluble vascular cell adhesion molecule-1 (s-VCAM-1), soluble platelet selectin (s-P-selectin), and s-CD44 were studied to determine if they could be used alone or in different combinations as markers for specific diagnostic procedures. Comparisons were made between congenitally, acutely, and chronically infected patients and aged-matched, noninfected individuals, as well as between patients with chronic Chagas' disease grouped according to the severity of their heart-related pathology. No differences in levels of s-CAMs were detected between sera from children with congenital T. cruzi infection and sera from noninfected infants born from chagasic mothers. In contrast, titers of s-ICAM-1, s-VCAM-1, s-selectin, and s-CD44 but not s-P-selectin were significantly increased in sera from patients during the acute phase of infection with T. cruzi. Titers of s-VCAM-1 and s-P-selectin were increased in chronically infected patients. A positive association with disease severity in sera from patients with chronic disease was observed for the levels of s-P-selectin. In contrast, we found no association between clinical symptoms and levels of s-VCAM-1. Patients with chronic disease with severe cardiopathy also showed diminished levels of s-CD44 in comparison with healthy controls or patients with mild disease. The results are discussed in the context of pathology of Chagas' disease.
Asunto(s)
Moléculas de Adhesión Celular/sangre , Enfermedad de Chagas/patología , Enfermedad Aguda , Adolescente , Adulto , Anciano , Moléculas de Adhesión Celular/química , Enfermedad de Chagas/sangre , Enfermedad de Chagas/congénito , Enfermedad Crónica , Selectina E/sangre , Selectina E/química , Humanos , Receptores de Hialuranos/sangre , Receptores de Hialuranos/química , Lactante , Molécula 1 de Adhesión Intercelular/sangre , Molécula 1 de Adhesión Intercelular/química , Persona de Mediana Edad , Selectina-P/sangre , Selectina-P/química , Índice de Severidad de la Enfermedad , Solubilidad , Molécula 1 de Adhesión Celular Vascular/sangre , Molécula 1 de Adhesión Celular Vascular/químicaRESUMEN
La enfermedad de Chagas se asocia con diversos trastornos inmunológicos. La participación del sistema inmune del huésped infectado en la resistencia a Trypanosoma cruzi es bien conocida, no así su papel patogénico. En esta dinámica, las citoquinas juegan un papel fundamental ya que su actividad determina el inicio, duración y composición de la respuesta inmune. El interferón-gamma (IFN-t) es la citoquina más relacionada con la resistencia a T. cruzi dada su capacidad para activar a los macrófagos cuya actividad tripanocida se correlaciona con la producción de especies reactivas del oxígeno (ROS) y óxido nítrico (NO). Otras citoquinas como las interleuquinas 4 (IL-4) y 10 (IL-10) y el factor de crecimiento y transformación beta (TGF-ß) inhibirían los efectos del IFN-t durante la infección con T. cruzi, ya que desactivan la capacidad tripanocida de macrófagos e inhiben la liberación de NO. Durante la infección con T. cruzi, el factor de necrosis tumoral alfa (TNF-a) se ha asociado tanto a la resistencia como al desarrollo de patología mientras que las interleuquinas 1 (IL-1) y 6 (IL-6) se han relacionado con alteraciones del endotelio vascular responsables del espasmo microvascular observado en la miocardiopatía chagásica. Diversas citoquinas inducen la expresión de moléculas de adhesión que contribuirían al desarrollo de las procesos inflamatorios a través de las interacciones celulares del sistema inmune y de éste con las células blanco de los tejidos. En la infección aguda con distintas cepas de T. cruzi se demostró la expresión de la molécula de adhesión intercelular 1 (ICAM-1) en miocardiocitos y leucocitos inflamatorios en forma paralela a la producción de citoquinas proinflamatorias. Los resultados descriptos en modelos experimentales muestran que la infección con T. cruzi induce la producción de citoquinas cuyas actividades biológicas regulan la resistencia al parásito y posiblemente la patología de la forma crónica de la enfermedad de Chagas. Más aún, el delicado balance entre citoquinas determinaría el curso de la infección ya que los mismos mecanismo involucrados en la resistencia participarían en el desarrollo de patología y la enfermedad se apoyaría en una alteración del equilibrio regulatorio de la respuesta inmune
Asunto(s)
Moléculas de Adhesión Celular , Enfermedad de Chagas/metabolismo , Suecia , Trypanosoma cruzi/inmunología , Trypanosoma cruzi/patogenicidad , ArgentinaRESUMEN
La enfermedad de Chagas se asocia con diversos trastornos inmunológicos. La participación del sistema inmune del huésped infectado en la resistencia a Trypanosoma cruzi es bien conocida, no así su papel patogénico. En esta dinámica, las citoquinas juegan un papel fundamental ya que su actividad determina el inicio, duración y composición de la respuesta inmune. El interferón-gamma (IFN-t) es la citoquina más relacionada con la resistencia a T. cruzi dada su capacidad para activar a los macrófagos cuya actividad tripanocida se correlaciona con la producción de especies reactivas del oxígeno (ROS) y óxido nítrico (NO). Otras citoquinas como las interleuquinas 4 (IL-4) y 10 (IL-10) y el factor de crecimiento y transformación beta (TGF-ß) inhibirían los efectos del IFN-t durante la infección con T. cruzi, ya que desactivan la capacidad tripanocida de macrófagos e inhiben la liberación de NO. Durante la infección con T. cruzi, el factor de necrosis tumoral alfa (TNF-a) se ha asociado tanto a la resistencia como al desarrollo de patología mientras que las interleuquinas 1 (IL-1) y 6 (IL-6) se han relacionado con alteraciones del endotelio vascular responsables del espasmo microvascular observado en la miocardiopatía chagásica. Diversas citoquinas inducen la expresión de moléculas de adhesión que contribuirían al desarrollo de las procesos inflamatorios a través de las interacciones celulares del sistema inmune y de éste con las células blanco de los tejidos. En la infección aguda con distintas cepas de T. cruzi se demostró la expresión de la molécula de adhesión intercelular 1 (ICAM-1) en miocardiocitos y leucocitos inflamatorios en forma paralela a la producción de citoquinas proinflamatorias. Los resultados descriptos en modelos experimentales muestran que la infección con T. cruzi induce la producción de citoquinas cuyas actividades biológicas regulan la resistencia al parásito y posiblemente la patología de la forma crónica de la enfermedad de Chagas. Más aún, el delicado balance entre citoquinas determinaría el curso de la infección ya que los mismos mecanismo involucrados en la resistencia participarían en el desarrollo de patología y la enfermedad se apoyaría en una alteración del equilibrio regulatorio de la respuesta inmune (AU)
Asunto(s)
Trypanosoma cruzi/inmunología , Trypanosoma cruzi/patogenicidad , Enfermedad de Chagas/metabolismo , Moléculas de Adhesión Celular , Suecia , ArgentinaRESUMEN
In the present study we demonstrate that spleens and hearts from BALB/c mice infected with the virulent Tulahuén or the low virulent CA-I strains of Trypanosoma cruzi, contain substantially higher ICAM-1 transcripts than uninfected controls. ICAM-1 expression in heart cells was also increased in the protein level, as measured by flow cytometry, ELISA and immunohistochemistry. The adhesive receptor was observed not only on inflammatory cells but also on sarcolemma of cardiac myocytes from T. cruzi infected mice. ICAM-1 expression was higher during the acute phase than in the chronic phase of infection, and paralleled the density of inflammatory leukocytes. Elevated titres of soluble ICAM-1 (s-ICAM-1) were detected in sera from mice during the acute phase of infection with CA-I or Tulahuén parasites. Cytokines, including IFN-gamma, IL-1 alpha, IL-6 and TNF-alpha have been shown to modulate expression of ICAM-1. Spleens and hearts from mice infected with CA-I or Tulahuen strains showed increased accumulation of mRNAs specific for these cytokines, which peaked during the acute phase of infection. However, IFN-gamma activity was not necessary for ICAM-1 induction, as its levels were also increased during infection in IFN-gamma receptor knock-out (IFN-gamma R- ) mice. Upregulation of ICAM-1 expression might be a direct consequence of parasite infection, since its density on cell lines of different lineages was enhanced after 24 or 48 h of infection with T. cruzi.