RESUMEN
Essential oil from Cymbopogon nardus was evaluated for activity against Trypanosoma brucei brucei BS221 (IC50 = 0.31 ± 0.03 µg/mL) and cytotoxic effect on normal kidney (Vero) cells (IC50 = >100 µg/mL). The crude essential oil was subjected to various chromatography techniques afforded active sub fractions with antitrypanosomal activity; F4 (IC50 = 0.61 ± 0.06 µg/mL), F6 (IC50= 0.73 ± 0.33 µg/mL), F7 (IC50 = 1.15 ± 0 µg/mL) and F8 (IC50 = 1.11 ± 0.01 µg/mL). These active fractions did not exhibit any toxic effects against Vero cell lines and the chemical profiles investigation indicated presence of α-and γ-eudesmol, elemol, α-cadinol and eugenol by GC/MS analysis.
Asunto(s)
Antiprotozoarios/farmacología , Cymbopogon/química , Aceites Volátiles/farmacología , Extractos Vegetales/farmacología , Trypanosoma brucei brucei/efectos de los fármacos , Animales , Antiprotozoarios/química , Antiprotozoarios/aislamiento & purificación , Antiprotozoarios/toxicidad , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Células Epiteliales/efectos de los fármacos , Células Epiteliales/fisiología , Cromatografía de Gases y Espectrometría de Masas , Concentración 50 Inhibidora , Aceites Volátiles/química , Aceites Volátiles/aislamiento & purificación , Aceites Volátiles/toxicidad , Pruebas de Sensibilidad Parasitaria , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/toxicidad , Células VeroRESUMEN
Essential oil from Cymbopogon nardus was evaluated for activity against Trypanosoma brucei brucei BS221 (IC50 = 0.31 ± 0.03 μg/mL) and cytotoxic effect on normal kidney (Vero) cells (IC50 = >100 μg/mL). The crude essential oil was subjected to various chromatography techniques afforded active sub fractions with antitrypanosomal activity; F4 (IC50 = 0.61 ± 0.06 μg/mL), F6 (IC50= 0.73 ± 0.33 μg/mL), F7 (IC50 = 1.15 ± 0 μg/mL) and F8 (IC50 = 1.11 ± 0.01 μg/mL). These active fractions did not exhibit any toxic effects against Vero cell lines and the chemical profiles investigation indicated presence of α-and γ-eudesmol, elemol, α-cadinol and eugenol by GC/MS analysis.