RESUMEN
Blackleg disease, a major threat to Brassica crops worldwide, is primarily caused by the pathogen Leptosphaeria biglobosa. Investigating the genetic variation of L. biglobosa is crucial for managing and preventing the disease in Brassica napus. To date, there is scarce genomic variation information available for populations of L. biglobosa in China. In this study, 73 L. biglobosa strains of canola stalks were collected from 12 provinces in China and subjected to re-sequencing. The 73 assemblies averaged 1340 contigs, 72,123 bp N50, and 30.17 Mb in size. In total, 9409 core orthogroups and 867 accessory orthogroups were identified. A total of 727,724 mutation loci were identified, including 695,230 SNPs and 32,494 indels. Principal component analysis (PCA) and population structure analysis showed that these strains could be divided into seven subgroups. The strains in most provinces were clustered into a single subgroup, suggesting a strong influence of the geographic environment on strain variation. The average nucleotide diversity (θπ) of all strains was 1.03 × 10-3, indicating important genetic diversity among strains from different regions of China. This study provides valuable resources for future comparative genomics, gives new insights into the population evolution of L. biglobosa, and supports the development of strategies for managing blackleg disease in canola.
RESUMEN
Infection by the clubroot pathogen Plasmodiophora brassicae on resistant and susceptible canola cultivars was investigated at various times following inoculation. Primary infection occurred on more than 90% of root hairs in both cultivars at 7 days after inoculation (dai), and thereafter declined to less than 20% at 14 to 35 dai. The amount of primary infection on the two cultivars was similar at each time point. Secondary infections were rare in both cultivars at 5 and 7 dai but became common after 14 dai. At 14 to 28 dai, the level of secondary infection was greater in the resistant cultivar than in the susceptible one. The in planta expression of 12 selected P. brassicae genes was investigated by reverse-transcription quantitative polymerase chain reaction. All genes were upregulated at 5 or 7 dai in the resistant cultivar. In the susceptible cultivar, the 12 genes could be classified into three groups according to their expression patterns: 2 genes showed an expression peak at 14 dai, 3 showed two expression peaks at 14 and 35 dai, and the others showed an expression peak at 35 dai. Results from this study will be useful in breeding for resistance and in selecting candidate pathogenicity genes for further studies.