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1.
Foot Ankle Int ; 22(11): 905-7, 2001 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11722144

RESUMEN

This paper is a radiographic approach to ascertain if the hardware at the medial malleolus is intra-articular. A human tibia was removed from a skeleton and pins were placed in the medial malleolus representing pins that would be intra- and extra-articular (in the mortise). Radiographs were obtained at precise rotation intervals. Those pins that were placed intra-articularly could not be made to appear otherwise. The pin that was placed extra-articularly was visualized on a single view to be extra-articular. Therefore, if hardware can be demonstrated to be extra-articular on any one view then it is not intra-articular.


Asunto(s)
Articulación del Tobillo/diagnóstico por imagen , Articulación del Tobillo/cirugía , Clavos Ortopédicos , Cadáver , Humanos , Radiografía/métodos
2.
J Biol Chem ; 263(7): 3432-9, 1988 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-2830285

RESUMEN

Reaction parameters were modified to enhance the in vitro reaction rate and to reveal partial and novel reactions of the group II intron 5g of the mitochondrial gene from Saccharomyces cerevisiae encoding cytochrome c oxidase subunit I. One alteration yields separate 5'- and 3'-exons plus linear excised intron as the main products. A linear reaction intermediate, containing intron and 3'-exon, and products resulting from cleavages at two unexpected sites were identified. Spliced exon "reopening," a novel reaction between excised intron and spliced exons, appears responsible for separate 5'- and 3'-exon products.


Asunto(s)
ADN de Hongos/genética , ADN Mitocondrial/genética , Complejo IV de Transporte de Electrones/genética , Genes Fúngicos , Intrones , Empalme del ARN , Saccharomyces cerevisiae/genética , Sulfato de Amonio/farmacología , Exones , Magnesio/farmacología , Cloruro de Magnesio , Cloruro de Potasio/farmacología , ARN de Hongos/genética , Saccharomyces cerevisiae/enzimología , Cloruro de Sodio/farmacología
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