RESUMEN
MicroRNAs are small non-coding RNAs involved in the regulation of gene expression in eukaryotes. Being transcribed in the nucleus in the form of a long primary precursor a primary microRNA undergoes multistep biogenesis that leads to the formation of a mature microRNA. One of the critical biogenesis steps is the recognition and processing of primary microRNAs by microprocessor complex. A primary microRNA secondary structure is considered to play a key role during this biogenesis step, but specific structural motives have not been identified yet. In the result of the study on structural and thermodynamic features of intergenic and intronic microRNAs we have demonstrated that the two main classes of human microRNAs have no statistically significant difference in respect of these parameters. The conclusion maybe a base for combining the two class microRNA sequences in a common set for the search for new microRNAs. Specific structural and thermodynamic features in human primary microRNA transcript hairpins have been found; the data may be used for search of new microRNA candidates in human genome and for a design of artificial microRNA sequences for gene therapy and functional genomics purposes.
Asunto(s)
ADN Intergénico/química , Intrones , MicroARNs/química , Conformación de Ácido Nucleico , Termodinámica , HumanosRESUMEN
Physiological and biochemical traits of epiphytic spore forming bacteria Bacillus pumilis BIM V-263 were examined. The nutrient medium and conditions for submerged cultivation of the strain were selected. The growth dynamics and antagonistic activity during cultivation in a laboratory fermenter ANKUM-2M were studied. The results provide grounds for development of the biological preparation Enatin with broad-range antimicrobial effect. The plant-protective and growth-stimulating effect of Enatin was examined in laboratory and field experiments. The preparation holds promise as means for biological control of crop pathogens.
Asunto(s)
Antiinfecciosos/farmacología , Bacillus/metabolismo , Antibacterianos , Antibiosis , Bacillus/crecimiento & desarrollo , Medios de Cultivo , Hongos/efectos de los fármacos , Hongos/crecimiento & desarrollo , Concentración de Iones de Hidrógeno , Cetonas/aislamiento & purificación , Cetonas/farmacología , Metanol , Desarrollo de la Planta , Plantas/efectos de los fármacos , Plantas/microbiología , TemperaturaRESUMEN
A nutritional additive eikonol containing polyunsaturated fatty acids omega-3 was given for a month (8 g/day) to correct vascular complications in 60 patients with non-insulin-dependent diabetes mellitus (NIDDM). Eikonol-treated NIDDM patients with diabetic retinopathy, polyneuropathy, angiopathy of the legs combining with arterial hypertension exhibited a reduction in the levels of total cholesterol, triglycerides, low and very low density lipoproteins cholesterol, a rise in concentrations of high density lipoproteins cholesterol. Blood pressure went down, peripheral vascular resistance and microhemocirculation improved. The findings evidence prophylactic and therapeutic efficacy of eikonol in vascular complications of NIDDM.
Asunto(s)
Diabetes Mellitus Tipo 2/tratamiento farmacológico , Grasas Insaturadas en la Dieta/administración & dosificación , Aceites de Pescado/administración & dosificación , Hipolipemiantes/administración & dosificación , Anciano , Enfermedad Crónica , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/complicaciones , Diabetes Mellitus Tipo 2/fisiopatología , Diabetes Mellitus Tipo 2/psicología , Evaluación de Medicamentos , Quimioterapia Combinada , Femenino , Hemodinámica/efectos de los fármacos , Hemostasis/efectos de los fármacos , Humanos , Lípidos/sangre , Masculino , Persona de Mediana EdadRESUMEN
Cardiovascular disease is the principal cause of morbidity, functional disability and fatal outcomes in patients with non-insulin-dependent diabetes mellitus (NIDDM). The results of administration of eikonol, food additive containing omega-3 unsaturated fatty acids, for 3 months were analyzed for 60 elderly NIDDM patients with ischemic heart disease. A single administration of eikonol leads to a decrease in accumulation of intracellular cholesterol by 15%. Diet eikonol decreased the level of total cholesterol and triglycerides in the blood by 23 and 43%, respectively. Moreover, the higher is the initial value of these parameters, the higher is the effect of the preparation. Eikonol proved to increase the level of lipoproteins of high density, produced a positive trends in NIDDM clinical picture. Food additive eikonol is recommended for wide administration in elderly NIDDM patients with atherogenic dyslipidemias.