RESUMEN

The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit software. Seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.

Asunto(s)

Infecciones por Rickettsia/epidemiología , Rickettsia rickettsii/aislamiento & purificación , Rickettsia/aislamiento & purificación , Coagulación Sanguínea , Brasil/epidemiología , Cartilla de ADN , Humanos , Reacción en Cadena de la Polimerasa , Reproducibilidad de los Resultados , Rickettsia/genética , Infecciones por Rickettsia/sangre , Rickettsia rickettsii/genética , Fiebre Maculosa de las Montañas Rocosas/sangre , Fiebre Maculosa de las Montañas Rocosas/epidemiología