RESUMEN

Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/REF-1) is a multifunctional protein acting on cellular signaling pathways, including DNA repair and redox activities. APE1/REF-1 has emerged as a target for cancer therapy, and its role in breast cancer models would reveal new strategies for cancer therapy. APX2009 is a specific APE1/REF-1 redox inhibitor whose anticancer properties have not been described in breast cancer cells. Here, we investigated the effect of the APX2009 treatment in the breast cancer cell lines MDA-MB-231 and MCF-7. Breast cancer cell lines were cultured, and WST1 and colony formation assays were performed to evaluate cell proliferation. Annexin V-FITC/7-AAD and LDH-Glo™ assays were performed to evaluate cell death. The wound healing assay and Matrigel transwell assay were performed after APX2009 treatment to evaluate the cellular migration and invasion processes, respectively. Our findings demonstrated that APX2009 treatment decreased breast cancer cell proliferative, migratory, and invasive properties. Furthermore, it induced apoptosis in both cell lines. Our study is the first to show the effects of APX2009 treatment on apoptosis in a breast cancer cell. Therefore, this study suggested that APX2009 treatment is a promising anticancer molecule for breast cancer.

Asunto(s)

Apoptosis , Neoplasias de la Mama , Movimiento Celular , Proliferación Celular , ADN-(Sitio Apurínico o Apirimidínico) Liasa , Oxidación-Reducción , Humanos , Neoplasias de la Mama/patología , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/metabolismo , ADN-(Sitio Apurínico o Apirimidínico) Liasa/metabolismo , ADN-(Sitio Apurínico o Apirimidínico) Liasa/antagonistas & inhibidores , Proliferación Celular/efectos de los fármacos , Femenino , Movimiento Celular/efectos de los fármacos , Línea Celular Tumoral , Apoptosis/efectos de los fármacos , Oxidación-Reducción/efectos de los fármacos , Fenotipo , Células MCF-7 , Antineoplásicos/farmacología

RESUMEN

Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/REF-1) is a multifunctional protein acting on cellular signaling pathways, including DNA repair and redox activities. APE1/REF-1 has emerged as a target for cancer therapy, and its role in breast cancer models would reveal new strategies for cancer therapy. APX2009 is a specific APE1/REF-1 redox inhibitor whose anticancer properties have not been described in breast cancer cells. Here, we investigated the effect of the APX2009 treatment in the breast cancer cell lines MDA-MB-231 and MCF-7. Breast cancer cell lines were cultured, and WST1 and colony formation assays were performed to evaluate cell proliferation. Annexin V-FITC/7-AAD and LDH-Glo™ assays were performed to evaluate cell death. The wound healing assay and Matrigel transwell assay were performed after APX2009 treatment to evaluate the cellular migration and invasion processes, respectively. Our findings demonstrated that APX2009 treatment decreased breast cancer cell proliferative, migratory, and invasive properties. Furthermore, it induced apoptosis in both cell lines. Our study is the first to show the effects of APX2009 treatment on apoptosis in a breast cancer cell. Therefore, this study suggested that APX2009 treatment is a promising anticancer molecule for breast cancer.

RESUMEN

Toxic baits, widely used against insect pests, are being successfully used to control mosquito vectors. In the present study, basic aspects for Attractive Toxic Sugar Baits (ATSBs) use as a control tool against Aedes aegypti including insecticide dosage, bait composition and plant application under laboratory conditions were evaluated. The Lethal Concentrations (LC 50 and 90) of boric acid (insecticide) Ae. aegypti engorgement and mortality were determined using ATSBs prepared using fruits (guava, mango and cupuaçu) and offered to mosquitoes on cotton discs and also sprayed on a Kalanchoe blossfeldiana plant. LCs of Ae. aegypti males and females did not differ significantly and varied from 0.53 to 2.46%, decreasing from 24 to 48 hours. No significant difference in the proportion of engorged male mosquitoes in ATSB (0.60) and Attractive Sugar Bait (ASB) (0.65) was found, but females engorged more on ASB (control bait) (0.80) compared to ATSBs (0.67). General mortality rate of mosquitoes in ATSB and ASB were 0.81 and 0.10 for males, respectively; 0.61 and 0.12 for females, respectively. Fruit composition affected neither engorgement nor mortality. ATSB applied on plants caused the mortality of males and females ranging from 0.75-0.87 while mortality on ASB sprayed plants varied from 0.07-0.14. Different common fruit juices and a low toxic oral insecticide are readily accepted, engorged and causes a high mortality both males and females Ae. aegypti using ATSBs. Moreover, the use of a common indoor plant in the region sprayed with ATSB under laboratory conditions leads to significant mosquito mortality.