RESUMEN
In the present work, we evaluated the effect of mixed Trypanosoma cruzi infections, studying the biological distribution of the different parasites in blood, heart and skeletal muscle during the acute phase. Albino Swiss mice were infected with different parasite strain/isolates or with a combination of them. The parasites in the different tissues were typified through specific PCR, population variability was analyzed through RFLP studies and parasitological and histopathological parameters were evaluated. We found a predominance of TcII and TcVI in all tissues samples respect to TcV and different parasite populations were found in circulation and in the tissues from the same host. These results verify the distribution of parasites in host tissues from early stages of infection and show biological interactions among different genotypes and populations of T. cruzi.
Asunto(s)
Enfermedad de Chagas/parasitología , Trypanosoma cruzi/fisiología , Animales , Enfermedad de Chagas/sangre , Enfermedad de Chagas/patología , Femenino , Genotipo , Corazón/parasitología , Humanos , Masculino , Ratones , Músculo Esquelético/parasitología , Músculo Esquelético/patología , Reacción en Cadena de la Polimerasa , Distribución Tisular , Trypanosoma cruzi/genética , Trypanosoma cruzi/crecimiento & desarrolloRESUMEN
Alternative strategies are being designed to identify candidates among drugs already available on the market that could be used in combination to improve the efficacy of Chagas disease treatment. This work evaluates the effect of the association of clomipramine (CLO) with benznidazole (BZN) for the treatment of experimental Chagas disease in the acute stage, in Swiss albino mice infected with Trypanosoma cruzi Tulahuen strain. Infected mice were treated with CLO 5mg/kg/day and BZN 50 and 100mg/kg/day, each separately or together. Efficacy of the treatment was evaluated through parasitemia, survival, electrocardiography, histopathological studies, serological and PCR assays at 90 days post-infection (dpi). All treatments significantly (P<0.05) reduced mortality and decreased parasitemia. Histopathological analysis of liver and kidneys of mice treated with CLO and the drug combination showed less injury than mice treated only with BZN. The lower dose of BZN (50mg/kg/day) combined with CLO showed the same efficacy as the habitual dose of BZN (100mg/kg/day) combined with CLO. The therapeutic results from the combination of BZN with CLO presented lesser side effects than the treatment with BZN.
Asunto(s)
Enfermedad de Chagas/tratamiento farmacológico , Clomipramina/uso terapéutico , Nitroimidazoles/uso terapéutico , Tripanocidas/uso terapéutico , Trypanosoma cruzi/efectos de los fármacos , Animales , Enfermedad de Chagas/patología , Clomipramina/farmacología , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Electrocardiografía , Femenino , Intestinos/patología , Riñón/patología , Hígado/patología , Masculino , Ratones , Músculo Esquelético/patología , Miocardio/patología , Nitroimidazoles/farmacología , Parasitemia , Tripanocidas/farmacologíaRESUMEN
Las enfermedades cardiovasculares constituyen laprincipal causa de muerte de la población adulta en la mayoríade los países. Los factores de riesgo estarían presentes enedades cada vez más tempranas. Se estudió 120 estudiantesde ambos sexos, entre 17 a 21 años de edad, ingresantes ala Facultad de Medicina de la Universidad Nacional de LaRioja en el año 2007. El 35 % no presentó presión sistólicanormal. Un 23,5 % posee un Indice de Masa Corporal porarriba de lo normal. Solamente un 19 % de las mujeresrealizan alguna actividad física. Un 66,7 % de las mujeresy un 50 % de los hombres poseen niveles de colesterol porarriba de los aceptables y el 74 % de los hombres tienen riesgocardiovascular moderado a elevado. La prevalencia observadade factores de riesgo cardiovascular representa un toque deatención dada la juventud de la población considerada(AU)
Cardiovascular diseases are the leading cause of death ofadult population in most countries. Risk factors seem to bepresent at increasingly early ages. We studied 120 studentsof both sexes between 17 and 21 years old, freshman at the School of Medicine, National University of La Rioja in 2007. Thirty-five percent do not show normal systolic pressure. Twenty-three point five percent have a body mass indexabove normal. Only 19% of women do some physical activity. Sixty-six point seven percentof women and 50% of men have cholesterol levels above the acceptable and 74% of menpresent moderate to high cardiovascular risk. The observed prevalence of cardiovascularrisk factors is a warning sign given the young age of the population studied(AU)
Asunto(s)
Adolescente , Adulto , Persona de Mediana Edad , Enfermedades Cardiovasculares/epidemiología , Prevalencia , Estudiantes , ArgentinaRESUMEN
Las enfermedades cardiovasculares constituyen laprincipal causa de muerte de la población adulta en la mayoríade los países. Los factores de riesgo estarían presentes enedades cada vez más tempranas. Se estudió 120 estudiantesde ambos sexos, entre 17 a 21 años de edad, ingresantes ala Facultad de Medicina de la Universidad Nacional de LaRioja en el año 2007. El 35 % no presentó presión sistólicanormal. Un 23,5 % posee un Índice de Masa Corporal porarriba de lo normal. Solamente un 19 % de las mujeresrealizan alguna actividad física. Un 66,7 % de las mujeresy un 50 % de los hombres poseen niveles de colesterol porarriba de los aceptables y el 74 % de los hombres tienen riesgocardiovascular moderado a elevado. La prevalencia observadade factores de riesgo cardiovascular representa un toque deatención dada la juventud de la población considerada
Cardiovascular diseases are the leading cause of death ofadult population in most countries. Risk factors seem to bepresent at increasingly early ages. We studied 120 studentsof both sexes between 17 and 21 years old, freshman at the School of Medicine, National University of La Rioja in 2007. Thirty-five percent do not show normal systolic pressure. Twenty-three point five percent have a body mass indexabove normal. Only 19% of women do some physical activity. Sixty-six point seven percentof women and 50% of men have cholesterol levels above the acceptable and 74% of menpresent moderate to high cardiovascular risk. The observed prevalence of cardiovascularrisk factors is a warning sign given the young age of the population studied