1.
Chem Commun (Camb)
; 57(1): 97-100, 2021 Jan 05.
Artículo
en Inglés
| MEDLINE
| ID: mdl-33332505
RESUMEN
A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.