1.
Chem Commun (Camb)
; 59(81): 12184-12187, 2023 Oct 10.
Artículo
en Inglés
| MEDLINE
| ID: mdl-37750315
RESUMEN
Accurate quantification of polymerized DNA in rolling circle amplification (RCA)-based hydrogels is challenging due to the high viscosity of these materials, however, it can be achieved with a photometric nucleotide depletion assay or qPCR. We show that the DNA content strongly depends on the template sequence and correlates with the mechanical properties of the hydrogels.