RESUMEN
Ten strains representing a single anamorphic novel yeast species were isolated from the external surface (DMKU-SP23 and DMKU-SP40) and tissue (DMKU-SE89, DMKU-SE99, DMKU-SE100 and DMKU-SE147) of sugarcane leaves in Thailand, and phylloplane (IMUFRJR 52034) and rhizoplane (IMUFRJ 52036 and 52037) of sugarcane and associated soil (IMUFRJ 52035) in Brazil. These strains showed zero to two nucleotide substitutions in the sequences of the D1/D2 region of the LSU rRNA gene and zero to three nucleotide substitutions in the internal transcribed spacer (ITS) region. Tremella globispora was the most closely related species, but with 1.7-2.1â% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 5.3-6.0â% nucleotide substitutions in the ITS region. Phylogenetic analysis based on the concatenated sequences of the ITS and the D1/D2 regions showed that these 10 strains represented a single species belonging to the genus Tremella (class Tremellomycetes, subphylum Agaricomycotina) that was distinct from related species. They therefore represented a novel species of the genus Tremella although the formation of basidia and basidiocarp were not observed. The name Tremella saccharicola f.a., sp. nov. is proposed. The type strain is DMKU-SP23T (=NBRC 109698T=BCC 61186T).
Asunto(s)
Basidiomycota/clasificación , Filogenia , Saccharum/microbiología , Microbiología del Suelo , Basidiomycota/aislamiento & purificación , Brasil , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Técnicas de Tipificación Micológica , Hojas de la Planta/microbiología , Rizosfera , Análisis de Secuencia de ADN , TailandiaRESUMEN
Nine strains representing a single anamorphic novel yeast species in dispersed tropical and subtropical habitats were isolated from sugarcane leaf tissue (DMKU-SE24, DMKU-SE45T, DMKU-SE129 and DMKU-SE134) and corn leaf tissue (DMKU-CE36) in Thailand, phylloplane and rhizoplane of sugarcane in Brazil (IMUFRJ 52018 and IMUFRJ 52019), bromeliad leaf tissue in Brazil (IMUFRJ 51954) and plant leaf in Japan (IPM31-24). These strains showed identical or only 1 nt substitution in the sequences of the D1/D2 region of the LSU rRNA gene and 0-5 nt substitutions in the internal transcribed spacer (ITS) region. Phylogenetic analysis based on the combined sequences of the ITS and the D1/D2 regions showed that the eight of these strains represented a single species in the genus Occultifur that was distinct from related species. Occultifur kilbournensis was the most closely related species, but with 0.9-1.2â% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 2.4-2.6â% nucleotide substitutions in the ITS region. They are therefore considered to represent a novel species of the genus Occultifur although the formation of basidia was not observed. The name Occultifur plantarum f.a., sp. nov. is proposed. The type strain is DMKU-SE45T (=CBS 14554T=TBRC 6561T).
Asunto(s)
Basidiomycota/clasificación , Filogenia , Saccharum/microbiología , Zea mays/microbiología , Composición de Base , Basidiomycota/genética , Basidiomycota/aislamiento & purificación , Brasil , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Japón , Técnicas de Tipificación Micológica , Hojas de la Planta/microbiología , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , TailandiaRESUMEN
Five strains representing a single novel anamorphic yeast species were isolated from sugar cane. Two strains were from tissue (DMKU-SE38, DMKU-SE59(T)) and two from the external surface (DMKU-SP385, DMKU-SP403) of leaves collected in Thailand and the fifth (IMUFRJ 52020) from the rhizoplane of sugar cane in an organically cultivated field in Brazil. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were classified as representing a single species of the genus Occultifur. The sequences of the D1/D2 region of the LSU rRNA genes and the ITS regions of the five strains were either identical or differed from each other by only one nucleotide substitution. The novel species was related most closely to Occultifur brasiliensis f.a. CBS 12687(T) but with 0.7-1.0% nucleotide substitutions (4-6 nt) in the D1/D2 region of the LSU rRNA gene and 2.5-2.7% nucleotide substitutions (14-15 nt) in the ITS region. The name Occultifur tropicalis f.a., sp. nov. is proposed. The type strain is DMKU-SE59(T) (â=BCC 61184(T)â=NBRC 109696(T)â=CBS 13389 (T)).
Asunto(s)
Basidiomycota/clasificación , Filogenia , Saccharum/microbiología , Basidiomycota/genética , Basidiomycota/aislamiento & purificación , Brasil , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Datos de Secuencia Molecular , Técnicas de Tipificación Micológica , Hojas de la Planta/microbiología , Análisis de Secuencia de ADN , Tailandia , Clima TropicalRESUMEN
Two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in Brazil and Taiwan. Analysis of the sequences of the D1/D2 domains of the large subunit rRNA gene showed that these novel species belong to the Wickerhamiella clade. The first was isolated from freshwater and a leaf of sugar cane (Saccharum officinarum) in Brazil and from leaves of Wedelia biflora in Taiwan. Described here as Wickerhamiella slavikovae sp. nov., it differs by 56 nucleotide substitutions and 19 gaps in the D1/D2 region of the large subunit rRNA gene from Candida sorbophila, the least divergent species. The second species, named Wickerhamiella goesii sp. nov., was isolated from leaves and the rhizosphere of sugar cane collected in Rio de Janeiro, Brazil. The species differs by 54 nucleotide substitutions and nine gaps in the D1/D2 domains from Candida drosophilae, its least divergent relative. The type strains are Wickerhamiella slavikovae sp. nov. IMUFRJ 52096(T) (= CBS 12417(T) = DBVPG 8032(T)) and Wickerhamiella goesii sp. nov. IMUFRJ 52102(T) (= CBS 12419(T) = DBVPG 8034(T)).