RESUMEN

A unique oligonucleotide pair, GOCC56:GOCC427, was designed that correctly primed specific amplification of a approximately 370-bp sequence spanning the ITS and 5.8S rDNA regions of Glomus occultum and Glomus brasilianum. In addition, this primer pair successfully detected G. occultum and G. brasilianum DNA in nested PCR using a primary PCR product amplified from highly diluted extracts of colonized corn (Zea mays) roots using modified ITS1:ITS4 primers. A second primer pair, GBRAS86:GBRAS388, primed specific amplification of a approximately 200-bp sequence spanning the ITS and 5.8S rDNA regions present only in G. brasilianum and Glomus strain GR582. Combined use of both primer pairs provides the means to detect and differentiate two ancient endomycorrhizal species, G. occultum and G. brasilianum, undetectable by standard root staining procedures. Sequence analysis showed that the purported G. occultum strain GR582 is likely a strain of G. brasilianum.

Asunto(s)

Cartilla de ADN , Hongos/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Secuencia de Bases , ADN de Hongos/análisis , ADN de Hongos/genética , ADN Ribosómico/análisis , ADN Ribosómico/genética , ADN Espaciador Ribosómico/análisis , ADN Espaciador Ribosómico/genética , Hongos/genética , Datos de Secuencia Molecular , Filogenia , Plantas/microbiología , Análisis de Secuencia , Simbiosis

Asunto(s)

Adyuvantes Inmunológicos , Formación de Anticuerpos , Vacunas , Animales , Encefalomielitis Equina , Cobayas , Pruebas de Inhibición de Hemaglutinación , Inmunoensayo , Isótopos de Yodo , Mercaptoetanol/farmacología , gammaglobulinas