RESUMEN
To investigate an outbreak of avian pox in psittacines in a conservation facility, we examined 94 birds of 10 psittacine species, including sick and healthy birds. We found psittacine pox virus in 23 of 27 sick birds and 4 of 67 healthy birds. Further characterization is needed for these isolates.
Asunto(s)
Avipoxvirus/genética , Enfermedades de las Aves/epidemiología , ADN Viral/genética , Brotes de Enfermedades , Infecciones por Poxviridae/veterinaria , Psittaciformes/virología , Animales , Avipoxvirus/clasificación , Avipoxvirus/aislamiento & purificación , Avipoxvirus/patogenicidad , Bioensayo , Enfermedades de las Aves/mortalidad , Enfermedades de las Aves/virología , Brasil/epidemiología , Embrión de Pollo , Membrana Corioalantoides/patología , Membrana Corioalantoides/virología , Conservación de los Recursos Naturales , Heces/virología , Filogenia , Reacción en Cadena de la Polimerasa , Infecciones por Poxviridae/epidemiología , Infecciones por Poxviridae/mortalidad , Infecciones por Poxviridae/virología , Piel/patología , Piel/virologíaRESUMEN
An outbreak of coccidiosis by Isospora icterus (I. icterus, Upton & Whitaker, 2000) in captive Campo Troupial (Icterus jamacaii) (Gmelin, 1788) at the Wild Animals Triage Center (IBAMA, Belo Horizonte, Brazil) is described. Clinical history and the necropsy findings documented diarrhea with diffuse necrotic enteritis. Sporulated oocysts (n = 100) had a bilayered wall, were subspherical, and measured 30.1 (27.5-32.5) microm in length and 28.5 (26.2-30.0) microm in width. A polar body but no micropyle was present and the length/width ratio was 1.1 (1.00-1.2). Each oocyst contained two ellipsoidal sporocysts measuring 17.6 (15.0-20.0) microm in length and 12.9 (12.5-15.0) microm in width, with a length/width ratio of 1.4 (1.2-1.5), and with Stieda and sub-Stieda bodies. Each sporocyst contained four sporozoites with granular sporocyst residuum. Oocysts were compatible with those from I. icterus, previously described in Campo Troupial.
Asunto(s)
Enfermedades de las Aves/parasitología , Coccidiosis/veterinaria , Isospora/aislamiento & purificación , Passeriformes , Animales , Enfermedades de las Aves/epidemiología , Brasil/epidemiología , Coccidiosis/epidemiología , Coccidiosis/parasitologíaRESUMEN
Borrelia anserina the agent of fowl spirochaetosis, has a worldwide distribution, where it is transmitted by Argas spp. ticks. The present study reports the first molecular characterization and in vitro isolation of an avian spirochaete strain from Brazil, presumably identified as B. anserina originated from naturally infected Argas miniatus ticks. DNA fragments of the rrs and flab genes were amplified by PCR and sequenced to determine phylogenetic similarities. The resulting sequences were 99.8% (483 of 484) and 98.7% (754 of 764) similar to GenBank corresponding sequences of B. anserina rrs and flaB genes, respectively. By neighbor-joining phylogenetic analysis, the flaB sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. anserina under 100% bootstrap support. The isolate was successfully isolated in BSK medium, with seven passages performed. The spirochaete strain isolated in the present study was genetically identified as B. anserina labeled as strain PL.
Asunto(s)
Infecciones por Borrelia/veterinaria , Borrelia/genética , Borrelia/aislamiento & purificación , Enfermedades de las Aves de Corral/microbiología , Animales , Infecciones por Borrelia/epidemiología , Infecciones por Borrelia/microbiología , Brasil/epidemiología , Pollos , Genes Bacterianos , Filogenia , Enfermedades de las Aves de Corral/epidemiología , Garrapatas/microbiologíaRESUMEN
Molecular analysis of 15 Brazilian infectious bronchitis virus (IBV) isolates, obtained from clinical outbreaks of the disease in chickens (broilers or layers) in the state of Minas Gerais (Brazil) between 1972 and 1989, is reported. Using the N protein gene as target, IBVs were analyzed by reverse transcription-polymerase chain reaction/restriction fragment length polymorphism (RT-PCR/RFLP) with the restriction enzymes AvaII, HphI, Sau96I, and Tsp509I and cDNA sequencing. Results obtained from those isolates were compared to 19 sequences available in GenBank. N gene RFLP profiles, cDNA sequences, and predicted amino acid composition were used for the construction of dendrograms. Brazilian isolates were grouped into one distinct group. Identity of predicted N protein amino acid composition varied from 45% (between isolates G and 208) up to 99% (PM 1 and PM2), and, when compared to the other IBVs, the amino acid identity was from 42% (Q3/88 and G) up to 97% (D41 and PM1). The great genetic diversity was shown to occur before the official use of vaccination in Brazil and has remained thereafter.