RESUMEN

A full length cDNA encoding a novel Trypanosoma cruzi DnaJ protein was cloned and characterized. The 324 amino acid protein encoded by the cDNA (TcDJ1) displays a characteristics J-domain, but lacks the Gly-Phe and zinc finger regions present in some other DnaJ proteins. Relative to four other T. cruzi DnaJ proteins, TcDJ1 has an amino terminal extension containing basic and hydroxylated resides characteristic of mitochondrial import peptides. A T. cruzi transfectant expressing epitope-tagged TcDJ1 was generated and subcellular fractions were produced. Western blot analysis revealed that the protein has a molecular mass of 29 kDa and is found in the mitochondrial fraction. The expression of TcDJ1 is developmentally regulated since the levels of both mRNA and protein are much higher in epimastigotes (replicative form) than in metacyclic trypomastigotes (infective form). Thus it may participate in mitochondrial biosynthetic processes in this organism.

Asunto(s)

Proteínas de Choque Térmico/genética , Proteínas Protozoarias/genética , Trypanosoma cruzi/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , ADN Complementario/genética , Regulación del Desarrollo de la Expresión Génica , Genes Protozoarios , Proteínas del Choque Térmico HSP40 , Proteínas de Choque Térmico/química , Proteínas de Choque Térmico/metabolismo , Mitocondrias/metabolismo , Datos de Secuencia Molecular , Peso Molecular , Proteínas Protozoarias/química , Proteínas Protozoarias/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Protozoario/genética , ARN Protozoario/metabolismo , Homología de Secuencia de Aminoácido , Trypanosoma cruzi/crecimiento & desarrollo , Trypanosoma cruzi/metabolismo