RESUMEN
MAP kinase kinase 1 (MKK1) is encoded by a single copy gene in Trypanosoma brucei. It has been shown recently that MKK1 is not essential for bloodstream forms [14]. To investigate the requirement for MKK1 in other life-cycle stages we generated null mutants in procyclic forms of a fly-transmissible strain. These grew normally in culture and were able to establish midgut infections in tsetse at normal rates and intensities, but were incapable of colonising the salivary glands. Transformation of null mutants with an ectopic copy of MKK1 enabled parasites to complete the life cycle in tsetse and infect mice. This is the first example of a gene that is indispensable for transmission of T. brucei. It also raises the possibility that activating the MKK1 signalling cascade in vitro might trigger the differentiation and proliferation of life-cycle stages of T. brucei that are currently refractory to culture.
Asunto(s)
Vectores de Enfermedades , MAP Quinasa Quinasa 1/metabolismo , Trypanosoma brucei brucei/enzimología , Tripanosomiasis Africana/transmisión , Moscas Tse-Tse/parasitología , Factores de Virulencia/metabolismo , Animales , Modelos Animales de Enfermedad , Eliminación de Gen , Prueba de Complementación Genética , Intestinos/parasitología , MAP Quinasa Quinasa 1/genética , Ratones , Glándulas Salivales/parasitología , Trypanosoma brucei brucei/genética , Factores de Virulencia/genéticaRESUMEN
The coat of Trypanosoma brucei consists mainly of glycosylphosphatidylinositol-anchored proteins that are present in several million copies and are characteristic of defined stages of the life cycle. While these major components of the coats of bloodstream forms and procyclic (insect midgut) forms are well characterised, very little is known about less abundant stage-regulated surface proteins and their roles in infection and transmission. By creating epitope-tagged versions of procyclic-specific surface antigen 2 (PSSA-2) we demonstrated that it is a membrane-spanning protein that is expressed by several different life cycle stages in tsetse flies, but not by parasites in the mammalian bloodstream. In common with other membrane-spanning proteins in T. brucei, PSSA-2 requires its cytoplasmic domain in order to exit the endoplasmic reticulum. Correct localisation of PSSA-2 requires phosphorylation of a cytoplasmic threonine residue (T(305)), a modification that depends on the presence of TbMAPK4. Mutation of T(305) to alanine (T(305)A) has no effect on the localisation of the protein in cells that express wild type PSSA-2. In contrast, this protein is largely intracellular when expressed in a null mutant background. A variant with a T(305)D mutation gives strong surface expression in both the wild type and null mutant, but slows growth of the cells, suggesting that it may function as a dominant negative mutant. The PSSA-2 null mutant exhibits no perceptible phenotype in culture and is fully competent at establishing midgut infections in tsetse, but is defective in colonising the salivary glands and the production of infectious metacyclic forms. Given the protein's structure and the effects of mutation of T(305) on proliferation and localisation, we postulate that PSSA-2 might sense and transmit signals that contribute to the parasite's decision to divide, differentiate or migrate.
Asunto(s)
Antígenos de Protozoos/metabolismo , Glicosilfosfatidilinositoles/química , Proteínas Protozoarias/metabolismo , Trypanosoma brucei brucei/metabolismo , Tripanosomiasis Africana/metabolismo , Glicoproteínas Variantes de Superficie de Trypanosoma/metabolismo , Animales , Antígenos de Protozoos/química , Ácido Aspártico/química , Citoplasma/metabolismo , Retículo Endoplásmico/metabolismo , Microscopía Fluorescente/métodos , Mutación , Fosforilación , Procesamiento Proteico-Postraduccional , Estructura Terciaria de Proteína , Proteínas Protozoarias/química , Moscas Tse-TseRESUMEN
Procyclic forms of Trypanosoma brucei reside in the midgut of tsetse flies where they are covered by several million copies of glycosylphosphatidylinositol-anchored proteins known as procyclins. It has been proposed that procyclins protect parasites against proteases and/or participate in tropism, directing them from the midgut to the salivary glands. There are four different procyclin genes, each subject to elaborate levels of regulation. To determine if procyclins are essential for survival and transmission of T. brucei, all four genes were deleted and parasite fitness was compared in vitro and in vivo. When co-cultured in vitro, the null mutant and wild type trypanosomes (tagged with cyan fluorescent protein) maintained a near-constant equilibrium. In contrast, when flies were infected with the same mixture, the null mutant was rapidly overgrown in the midgut, reflecting a reduction in fitness in vivo. Although the null mutant is patently defective in competition with procyclin-positive parasites, on its own it can complete the life cycle and generate infectious metacyclic forms. The procyclic form of T. brucei thus differs strikingly from the bloodstream form, which does not tolerate any perturbation of its variant surface glycoprotein coat, and from other parasites such as Plasmodium berghei, which requires the circumsporozoite protein for successful transmission to a new host.
Asunto(s)
Glicoproteínas de Membrana/fisiología , Proteínas Protozoarias/fisiología , Trypanosoma brucei brucei/química , Tripanosomiasis Africana/transmisión , Moscas Tse-Tse/parasitología , Animales , Animales Modificados Genéticamente , Técnicas de Cocultivo , Glicoproteínas de Membrana/genética , Proteínas Protozoarias/genéticaRESUMEN
A 'two coat' model of the life cycle of Trypanosoma brucei has prevailed for more than 15 years. Metacyclic forms transmitted by infected tsetse flies and mammalian bloodstream forms are covered by variant surface glycoproteins. All other life cycle stages were believed to have a procyclin coat, until it was shown recently that epimastigote forms in tsetse salivary glands express procyclin mRNAs without translating them. As epimastigote forms cannot be cultured, a procedure was devised to compare the transcriptomes of parasites in different fly tissues. Transcripts encoding a family of glycosylphosphatidyl inositol-anchored proteins, BARPs (previously called bloodstream alanine-rich proteins), were 20-fold more abundant in salivary gland than midgut (procyclic) trypanosomes. Anti-BARP antisera reacted strongly and exclusively with salivary gland parasites and a BARP 3' flanking region directed epimastigote-specific expression of reporter genes in the fly, but inhibited expression in bloodstream and procyclic forms. In contrast to an earlier report, we could not detect BARPs in bloodstream forms. We propose that BARPs form a stage-specific coat for epimastigote forms and suggest renaming them brucei alanine-rich proteins.
Asunto(s)
Alanina/química , Proteínas Protozoarias/química , Trypanosoma brucei brucei/química , Moscas Tse-Tse/parasitología , Glicoproteínas Variantes de Superficie de Trypanosoma/química , Secuencia de Aminoácidos , Animales , Proteínas Protozoarias/metabolismo , Moscas Tse-Tse/anatomía & histologíaRESUMEN
EP and GPEET procyclins are the major surface glycoproteins of Trypanosoma brucei in the midgut of tsetse flies (Glossina spp.). The procyclin genes are located at the beginning of polycistronic transcription units and are followed by at least one procyclin-associated gene (PAG). The EP/PAG1 locus on one copy of chromosome X begins with the three genes EP1, EP2 and PAG1; the end of this unit has not been characterized previously. The EP/PAG2 locus on the other copy of chromosome X contains the same procyclin genes followed by PAG2 and PAG4. Here we show that the EP/PAG1 locus in AnTat1.1 has to be extended by three more PAGs, which we named PAG5, PAG2* and PAG4. The EP/PAG2 locus most likely evolved from the EP/PAG1 locus by deletion of a fragment from within PAG1 to PAG2*. The procyclin loci on the two copies of chromosome VI are indistinguishable, and contain the genes GPEET, EP3, PAG3 and GRESAG2.1. The mRNA levels of PAG1, PAG2 and PAG3 are transiently increased during differentiation of bloodstream forms to procyclic forms. Unexpectedly, procyclic forms of a PAG knockout clone lacking all eight PAGs in the procyclin loci were transmissible by Glossina morsitans. Furthermore, the deletion mutant could still establish midgut infections when competing with a tagged clone with the full complement of PAGs. Cyclical transmission was also possible when tsetse flies were infected with bloodstream forms of the deletion mutant, demonstrating that the PAGs are not essential for the differentiation of bloodstream to procyclic forms in vivo.
Asunto(s)
Genes Protozoarios , Insectos Vectores/parasitología , Glicoproteínas de Membrana/genética , Proteínas Protozoarias/genética , Trypanosoma brucei brucei/genética , Tripanosomiasis/parasitología , Moscas Tse-Tse/parasitología , Animales , Secuencia de Bases , Mapeo Cromosómico , Femenino , Biblioteca de Genes , Genoma de Protozoos , Estadios del Ciclo de Vida , Ratones , Datos de Secuencia Molecular , Alineación de Secuencia , Trypanosoma brucei brucei/crecimiento & desarrollo , Tripanosomiasis/transmisiónRESUMEN
African trypanosomes undergo differentiation in order to adapt to the mammalian host and the tsetse fly vector. To characterize the role of a mitogen-activated protein (MAP) kinase homologue, TbMAPK5, in the differentiation of Trypanosoma brucei, we constructed a knockout in procyclic (insect) forms from a differentiation-competent (pleomorphic) stock. Two independent knockout clones proliferated normally in culture and were not essential for other life cycle stages in the fly. They were also able to infect immunosuppressed mice, but the peak parasitemia was 16-fold lower than that of the wild type. Differentiation of the proliferating long slender to the nonproliferating short stumpy bloodstream form is triggered by an autocrine factor, stumpy induction factor (SIF). The knockout differentiated prematurely in mice and in culture, suggestive of increased sensitivity to SIF. In contrast, a null mutant of a cell line refractory to SIF was able to proliferate normally. The differentiation phenotype was partially rescued by complementation with wild-type TbMAPK5 but exacerbated by introduction of a nonactivatable mutant form. Our results indicate a regulatory function for TbMAPK5 in the differentiation of bloodstream forms of T. brucei that might be exploitable as a target for chemotherapy against human sleeping sickness.
Asunto(s)
Patógenos Transmitidos por la Sangre , Estadios del Ciclo de Vida/fisiología , Proteínas Quinasas Activadas por Mitógenos/fisiología , Trypanosoma brucei brucei/fisiología , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Animales , Patógenos Transmitidos por la Sangre/aislamiento & purificación , Recuento de Células , Diferenciación Celular , Células Cultivadas , Ingeniería Genética , Ratones , Proteína Quinasa 7 Activada por Mitógenos/genética , Proteínas Quinasas Activadas por Mitógenos/genética , Datos de Secuencia Molecular , Mutación , Filogenia , Sensibilidad y Especificidad , Eliminación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
We previously showed that over-expression of Trypanosoma brucei MRPA, a member of the multidrug resistance protein family in T. brucei, reproducibly resulted in resistance to the anti-trypanosomal drug melarsoprol in vitro. MRPA is predicted to mediate efflux of melarsoprol as a conjugate with trypanothione, a glutathione-spermidine conjugate which is the major small thiol in trypanosomes. Here, we show that depletion of MRPA by RNA interference resulted in moderate hypersensitivity to both melarsoprol and melarsen oxide. Over-expression of MRPA alone is not sufficient to cause melarsoprol resistance in vivo, although it is sufficient in vitro. This discrepancy is not an effect of drug metabolism since over-expression of MRPA alone conferred resistance to melarsoprol and its principle metabolite, melarsen oxide, in vitro. Over-expression of MRPA was not detected in four melarsoprol-resistant trypanosome isolates from sleeping sickness patients.
Asunto(s)
Melarsoprol/farmacología , Proteínas de Transporte de Membrana/fisiología , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/fisiología , Proteínas Protozoarias/fisiología , Tripanocidas/farmacología , Trypanosoma brucei brucei/efectos de los fármacos , Tripanosomiasis Africana/parasitología , Animales , Arsenicales/farmacología , Western Blotting/métodos , Línea Celular , Electroforesis en Gel de Poliacrilamida , Femenino , Expresión Génica , Humanos , Melarsoprol/química , Melarsoprol/uso terapéutico , Proteínas de Transporte de Membrana/análisis , Proteínas de Transporte de Membrana/biosíntesis , Ratones , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/análisis , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/biosíntesis , Pruebas de Sensibilidad Parasitaria/métodos , Proteínas Protozoarias/análisis , Proteínas Protozoarias/biosíntesis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Insuficiencia del Tratamiento , Tripanocidas/química , Tripanocidas/uso terapéutico , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/metabolismo , Tripanosomiasis Africana/tratamiento farmacológicoRESUMEN
Trypanosoma brucei, the parasite causing human sleeping sickness, relies on the tsetse fly for its transmission. In the insect, EP and GPEET procyclins are the major surface glycoproteins of procyclic (midgut) forms of the parasite, with GPEET predominating in the early procyclic form and two isoforms of EP in the late procyclic form. EP procyclins were previously detected on salivary gland trypanosomes, presumably epimastigotes, by immunoelectron microscopy. However, no procyclins could be detected by mass spectrometry when parasites were isolated from infected glands. We have used qualitative and quantitative RT-PCR to analyse the procyclin mRNAs expressed by trypanosomes in the tsetse midgut and salivary glands at different time points after infection. The coding regions of the three EP isoforms (EP1, EP2 and EP3) are extremely similar, but their 3' untranslated regions contain unique sequences that make it possible to assign the cDNAs amplified by this technique. With the exception of EP2, we found that the spectrum of procyclin mRNAs expressed in the midgut mirrors the protein repertoire of early and established procyclic forms. Surprisingly, procyclin mRNAs, including that of GPEET, are present at relatively high levels in salivary gland trypanosomes, although the proteins are rarely detected by immunofluorescence. Additional experiments using transgenic trypanosomes expressing reporter genes or mutant forms of procyclin point to a mechanism of translational or post-translational control, involving the procyclin coding regions, in salivary gland trypanosomes. It is widely accepted that T. brucei always has a coat of either variant surface glycoprotein or procyclin. It has been known for many years that the epimastigote form does not have a variant surface glycoprotein coat. The finding that this life cycle stage is usually negative for procyclin as well is new, and means that the paradigm will need to be revised.
Asunto(s)
Glicoproteínas de Membrana/genética , Proteínas Protozoarias/genética , ARN Mensajero/metabolismo , Trypanosoma brucei brucei/genética , Tripanosomiasis Africana/transmisión , Moscas Tse-Tse/parasitología , Animales , Regulación del Desarrollo de la Expresión Génica , Genes Protozoarios/genética , Interacciones Huésped-Parásitos , Masculino , Ratones , Ratones Endogámicos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Tripanosomiasis Africana/parasitología , Moscas Tse-Tse/anatomía & histologíaAsunto(s)
Glicoproteínas de Membrana/química , Trypanosoma brucei brucei/química , Tripanosomiasis Africana/etiología , Tripanosomiasis Africana/parasitología , Secuencia de Aminoácidos , Animales , Sistema Digestivo/parasitología , Expresión Génica , Genes Protozoarios , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/fisiología , Datos de Secuencia Molecular , Procesamiento Proteico-Postraduccional , Estructura Terciaria de Proteína , Proteínas Protozoarias , Eliminación de Secuencia , Homología de Secuencia de Aminoácido , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/patogenicidad , Trypanosoma brucei brucei/fisiología , Moscas Tse-Tse/parasitologíaRESUMEN
In cycling between the mammalian host and the tsetse fly vector, trypanosomes undergo major changes in energy metabolism and surface coat composition. Early procyclic (insect) forms in the tsetse fly midgut are coated by glycoproteins known as EP and GPEET procyclins. EP expression continues in late procyclic forms, whereas GPEET is down-regulated. In culture, expression of GPEET is modulated by glycerol or glucose. Here, we demonstrate that a glycerol-responsive element of 25 nucleotides within the 3' untranslated region of GPEET mRNA also controls expression by glucose and during development in the fly. In trypanosomes, mitochondrial ATP is produced mainly by the acetate: succinate-CoA transferase/succinyl-CoA synthetase (ASCT) cycle, the citric acid cycle, and the cytochromes. Silencing of the pyruvate dehydrogenase or succinyl-CoA synthetase from the ASCT cycle by RNA interference induces reexpression of GPEET in late procyclic forms, whereas inhibition of the citric acid cycle or the cytochromes has no effect. In contrast, inhibition of the alternative oxidase, the second branch of the electron transport chain, with salicylhydroxamic acid overrides the effect of glucose or glycerol and causes a reduction in the level of GPEET mRNA. Our results reveal a new mechanism by which expression of a surface glycoprotein is controlled by the activity of mitochondrial enzymes.
Asunto(s)
Glicoproteínas de Membrana/genética , Proteínas Protozoarias/genética , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/metabolismo , Regiones no Traducidas 3' , Animales , Secuencia de Bases , ADN Protozoario/genética , Metabolismo Energético , Expresión Génica/efectos de los fármacos , Genes Protozoarios , Glucosa/farmacología , Glicerol/farmacología , Mitocondrias/enzimología , Modelos Biológicos , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Conformación de Ácido Nucleico , Interferencia de ARN , ARN Mensajero/química , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Protozoario/química , ARN Protozoario/genética , ARN Protozoario/metabolismo , Trypanosoma brucei brucei/crecimiento & desarrollo , Moscas Tse-Tse/parasitologíaRESUMEN
Procyclic culture forms of Trypanosoma congolense have been shown to express a glutamic acid/alanine-rich protein (GARP) on their surface. By labelling T. congolense procyclic culture forms with glycosylphosphatidylinositol (GPI) precursors, we show that GARP is bound to the membrane by a GPI anchor and demonstrate the presence of two additional GPI-anchored surface molecules of 24-34 and 58 kDa that are abundantly expressed. The 24-34 kDa molecule, which is recognised by monoclonal antibodies that bind to the surface of living trypanosomes, is resistant to proteolysis, suggesting that it consists (predominantly) of non-proteinaceous material. We have therefore named it protease-resistant surface molecule (PRS). In common with the EP and GPEET procyclins of Trypanosoma brucei, the relative expression of the T. congolense GPI-anchored molecules changes during parasite development in the tsetse fly. PRS is abundantly expressed by procyclic trypanosomes in the midgut shortly after infection, but is downregulated in established midgut forms and completely absent from the epimastigote form in the proboscis. In contrast, GARP is downregulated in parasites in the tsetse fly midgut, but upregulated in the epimastigote form. Unexpectedly, 14 days post-infection, procyclic forms frequently are negative for both PRS and GARP, suggesting that they might be expressing another stage-specific surface antigen at this point in the life cycle.