RESUMEN
In this study, we provided the detailed characterizations of our recent HRP-AuNPs/PEDOT:BSA/Pt biosensor, constructed through a simple fabrication procedure with improved stability and good sensitivity. Raman and Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy not only confirmed the synthesis of conductive PEDOT where BSA was the template for the polymerization, but also provided further insights into the stable immobilization of AuNP on the PEDOT:BSA film. Scanning electron microscopy revealed that the attachment of AuNPs were stable under a high salt environment. The current technology demonstrates a feasible procedure to form a functional AuNPs/PEDOT:BSA film that has potential applications in the fabrication of various biosensors and electric devices.
Asunto(s)
Técnicas Biosensibles/métodos , Técnicas Electroquímicas/métodos , Peroxidasa de Rábano Silvestre/metabolismo , Animales , Técnicas Biosensibles/instrumentación , Compuestos Bicíclicos Heterocíclicos con Puentes/química , Bovinos , Electrodos , Oro/química , Peróxido de Hidrógeno/análisis , Nanopartículas del Metal/química , Microscopía Electrónica de Transmisión , Espectroscopía de Fotoelectrones , Platino (Metal)/química , Polímeros/química , Albúmina Sérica Bovina/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP/AuNPs/PEDOT(BSA)/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide. Our results revealed a satisfied linear correlation between the cathodic current and the concentration of H2O2. Furthermore, the addition of oxidized form of nicotinamide adenine dinucleotide, or NADâº, as the electron transfer mediator in the detection solution could dramatically enhance the sensitivity of detection by about 35.5%. The main advantages of the current biosensor are its durability, sensitivity, reliability, and biocompatibility.