RESUMEN
Introduction Two cases of infection by zoonotic transmission of unusual species of Cryptosporidium were detected in 2010-2011 in Spain (León and Zaragoza).Materials and methods Cryptosporidium spp. was detected by microscopic examination of modified Ziehl-Neelsen stained fecal smears. PCR-RFLP of the SSUr DNA gene and sequencing of the amplified fragment confirmed the species. Results C. ubiquitum and C. felis were identified in samples from an immunocompetent child and from a HIV-positive adult, respectively. Conclusions This is the first report of human infection by C. ubiquitum (cervine) and autochthonous C. felis, identified in Spain (AU)
Introducción: Se describen dos casos de infección por especies inusuales de Cryptosporidium de transmisión zoonótica detectados en España (León y Zaragoza) en 2010 y 2011. Material y métodos: Cryptosporidium se detectó por tinción Ziehl-Neelsen modificada de la concentración de heces Las especies se determinaron por PCR-RFLP del gen SSUr DNA y se confirmaron por secuenciación del fragmento amplificado. Resultados: C.ubiquitum y C. felis fueron identificados en muestras procedentes de un niño inmunocompetente y un adulto VIH-positivo respectivamente. Conclusiones: Este estudio es la primera comunicación de infecciones humanas por C. ubiquitum (cervine)y de Cryptosporidium felis de origen autóctono identificados en España (AU)
Asunto(s)
Humanos , Masculino , Niño , Adulto , Criptosporidiosis/epidemiología , Cryptosporidium/aislamiento & purificación , Zoonosis/transmisión , Infecciones Oportunistas Relacionadas con el SIDA/diagnósticoRESUMEN
INTRODUCTION: Two cases of infection by zoonotic transmission of unusual species of Cryptosporidium were detected in 2010-2011 in Spain (León and Zaragoza). MATERIALS AND METHODS: Cryptosporidium spp. was detected by microscopic examination of modified Ziehl-Neelsen stained fecal smears. PCR-RFLP of the SSUrDNA gene and sequencing of the amplified fragment confirmed the species. RESULTS: C. ubiquitum and C. felis were identified in samples from an immunocompetent child and from a HIV-positive adult, respectively. CONCLUSIONS: This is the first report of human infection by C. ubiquitum (cervine) and autochthonous C. felis, identified in Spain.