RESUMEN
Feeding is a determining factor in the various characteristics of sheep meat and animal performance, the objectives were to evaluate the effect of supplementation of ewe lambs finished in different nutritional planes on the gene expression of CASP3, CAPN1, CAPN2 and CAST and its possible association with meat quality. Samples of the Longissimus lumborum muscle of 24 ewe lambs were used, distributed in 3 groups (n=8): P (pasture), PS (pasture and supplement) and F (feedlot). Physicochemical analyses were performed for centesimal analysis, pH, lipid oxidation, Warner-Bratzler shear force and RT-qPCR for the analysis of relative gene expression of the following genes: CASP3, CAPN1, CAPN2 and CAST. There is an increase in daily weight gain and ethereal extract values in the meat of confined animals, due to the greater energy intake in the nutrition of these animals. Animals kept only on pasture have lower lipid oxidation in meat than other treatments because of the lower percentage of lipids. The Warner-Bratzler shear force is considerably higher in the meat of animals kept only on pasture but is still considered tender. The different nutritional systems do not interfere with the gene expression of CASP3, CAPN1, CAPN2 and CAST in ewe lambs.
Asunto(s)
Carne Roja , Femenino , Animales , Ovinos/genética , Caspasa 3 , Carne/análisis , Expresión Génica , LípidosRESUMEN
Brucella ovis is the causative agent of ovine brucellosis, which is an important infectious disease in sheep farming worldwide and is responsible for economic losses because of its negative effect on the reproductive system of rams and ewes. Serologic tests are the main tools for detection of infection; however, these tests commonly yield a high frequency of false-negative results. We compared 2 serologic tests, agar gel immunodiffusion (AGID) and ELISA, for the detection of anti-B. ovis antibodies in naturally infected sheep. Of the 728 serum samples analyzed, 0.3% were positive by AGID and 9.2% by ELISA. Positive results were obtained for different animals and flocks. There was no statistical difference between the detection frequency of the 2 methods (p = 0.674), and the kappa test indicated low concordance (κ = 0.005). The lack of agreement between results obtained using AGID and ELISA, associated with the absence of clinical signs, makes it difficult to detect ovine brucellosis efficiently, and demonstrates the need for effective tests for the definitive detection of B. ovis infection.