RESUMEN

During the investigation of firearm-related incidents, gunshot residues (GSR) can be collected on the scene and individuals (e.g., shooters or bystanders). Their analysis can give valuable information for the reconstruction of the events. Since GSR collection on persons of interest generally occurs a few minutes to hours after discharge, knowledge is needed to understand how organic (O), and inorganic (I) residues are transferred and persist. In this research, the quantities of OGSR and IGSR were assessed on the right and left hands, forearms, face, and nostrils of four shooters. Specimens were collected immediately before the discharge (shooter's blank specimens) and shortly after (30 min) using carbon adhesive stubs. Organic compounds were first extracted from the collection device and analysed using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Subsequently, IGSR particles were detected on the same stub using scanning electron microscopy coupled with energy-dispersive X-ray spectrometry (SEM/EDS). Shooter's blank specimen analysis revealed background contamination of both O and IGSR in the shooter's environment, predominantly attributed to the presence of an indoor shooting range. However, the background quantities generally remained below the associated 30-minute specimen. Thirty minutes after a discharge, higher quantities were generally detected on the shooter's right and left hands than on other collection regions for both GSR types. Forearms and face emerged as interesting collection alternatives, especially in cases where a person of interest may have washed their hands in the interval between the discharge and collection. In contrast, very low amounts of GSR were detected in the nostrils. Furthermore, the results indicated that OGSR and IGSR have different transfer and persistence mechanisms.

Asunto(s)

Armas de Fuego , Balística Forense , Mano , Humanos , Balística Forense/métodos , Cara/anatomía & histología , Microscopía Electrónica de Rastreo , Espectrometría por Rayos X , Nariz , Antimonio/análisis , Antebrazo , Espectrometría de Masas en Tándem , Bario/análisis , Heridas por Arma de Fuego , Cromatografía Líquida de Alta Presión , Ciclohexanonas

RESUMEN

The influence of the drying position and time on the persistence of gunshot residues (GSR) on fabrics was investigated. Powder tattooing from a 40 cm shooting distance on targeted fabrics were slightly wetted using a spray and dried for 3 h or 48 h in horizontal or vertical positions. The GSR particles were visualised using IR fluorescence with a Video Spectral Comparator (VSC) and automatically counted with a software before the wetting and after the drying stages. While a significant rate of GSR particles (up to 22%) were lost, it was mainly due to the wetting procedure rather than the drying stage. No statistically significant difference was observed between the drying positions, indicating that both drying positions can be used in practice to dry slightly wet fabrics. The drying time had no influence on the loss, and the GSR distribution was not significantly influenced by either factor (i.e., time and position).

RESUMEN

Gunshot residues (GSR) collected during the investigation of firearm-related incidents can provide useful information for the reconstruction of the events. Two main types of GSR traces can be targeted by forensic scientists, the inorganic (IGSR) and the organic GSR (OGSR). Up to now, forensic laboratories have mainly focused on the detection of inorganic particles on the hands and clothes of a person of interest using carbon stubs analysed by scanning electron microscopy coupled with energy dispersive X-ray spectrometry (SEM/EDS). Several approaches have been proposed to also analyse the organic compounds since they might bring additional information for the investigation. However, implementing such approaches might disrupt the detection of IGSR (and vice versa depending on the applied sequence of analysis). In this work, two sequences were compared for the combined detection of both types of residues. One carbon stub was used for collection, and the analysis was performed either by targeting the IGSR or the OGSR first. The aim was to evaluate which one allows maximum recovery of both types of GSR while minimising losses that might occur at different stages of the analysis process. SEM/EDS was used for the detection of IGSR particles while an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was used for the analysis of OGSR compounds. Extracting OGSR first required the implementation of an extraction protocol that did not interfere with the IGSR particles present on the stub. Both sequences allowed good recovery of the inorganic particles since no significant difference was observed in the detected concentrations. However, OGSR concentrations were lower after IGSR analysis than before for two compounds (ethyl and methylcentralite). Thus, it is advised to extract rapidly the OGSR before or after IGSR analysis to avoid losses during the storage and analysis processes. The data also indicated that there was a low correlation between IGSR and OGSR highlighting the potential of a combined detection and analysis of both types of GSR.

RESUMEN

Gunshot residue (GSR) analysis and their interpretation provide crucial information on a criminal investigation involving the use of firearms. To date, several approaches have been proposed for the implementation of a combined sampling and analysis of inorganic (IGSR) and organic GSR (OGSR). However, it is not clear at this stage if concurrent analyses of both types of residue might be detrimental to the analysis of IGSR currently applied in forensic laboratories. Thus, this work aims to compare and evaluate three different protocols for the combined collection and analysis of IGSR and OGSR. These methods, respectively, involve the use of a modified stub (with two halves, one for the detection of IGSR and the other for the analysis of OGSR); the sequential recovery of GSR with two stubs mounted with different adhesives (double-sided carbon tape and Tesa® TACK) and the sequential analysis of IGSR and OGSR from a single carbon stub following carbon deposition. The detection of IGSR was carried out using SEM-EDX, while OGSR analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Obtained results for experiments performed using Geco Sinoxid® ammunition indicated that sequential analysis was the most suitable protocol for the combined collection and analysis of both IGSR and OGSR. A higher number of inorganic (characteristic and consistent) particles and higher concentrations of ethylcentralite, N-nitrosodiphenylamine, diphenylamine, and nitroglycerin were recovered with this method.